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The map label for this gene is oprM [H]

Identifier: 238024609

GI number: 238024609

Start: 1560234

End: 1561784

Strand: Direct

Name: oprM [H]

Synonym: bglu_2g12240

Alternate gene names: 238024609

Gene position: 1560234-1561784 (Clockwise)

Preceding gene: 238024608

Following gene: 238024613

Centisome position: 55.18

GC content: 76.47

Gene sequence:

>1551_bases
ATGACGCGCCGCTTCGCGCCCGCGGCCGGCCGCCCGCCGGCATCGCCATCGCGCCGCGCCGCCGCCATGGCGGCACTCTC
GGCGGCGCTGGTGCTCGCGGCCTGCACCACCGTCGGCCCCGACTACCGGCTGCCGAAGCAGGCACTCGTCAACGCGCCGC
TCGCCGGCGCCCCGCTAGACGGCGCCGACGACGGCCGCGCGCTCGTCTCGCACGAGGCGGTCCCGGCCCGCTGGTGGCGG
CTCTACGACGATCCCGTGCTCGACGCGCTGGTGCAGCAGGCGCTCGACTCCAACACCGATCTGCGCGTGGCCGCGGCGAA
CCTGGCGCGCGCGCGCGCCGCGCTCGAGGTCGAGAGCGCGCAGGGCGGCTTGACCGGCAATCTCGAGGGCGGTGCGCAGC
GCGCCCAGGAGTCGGCCGAGCAATACCTGCTGACGGACAAGCTGCCGGTGGTCAACGAAGGCGCGTTCAACTTCAACGTG
TCCTACGAGTTCGACCTGTTCGGCAAGCTGCGGCGCGGCGTCGAGGCCGCGCGCGCCGACCGCGAAGCCGTGAAGGCGGC
CGGCGATCTCGCGCGCATCACGGTGGTGGCCGAGGTCGTGCGCGCCTACGTGGAGGCCTGCTCGGCGGGCGAGGAACTCG
CGATCGCGAAGCGGTCGCTGGACCTGCAGCGCCAGCGCGTGGCGCTCTCGCGGCGGCTGCGCGACGCCGGCCGGGGCAGC
CAGCCCGAGGTCACGCGCGGCGTGGCGCAGGTGCAGACGCTGGCCGCCGACATCCCGCGCTTCGAGGGCCGTCGCCGCGT
CGCGCAATACCAGCTGGCCGCGCTGCTGGCGCGCTCGCCGGCCGAGCTGCCGGCCGCCGCGCACGGCTGCAGGCAGTTGC
CGAGCCTGAAGCAGGCGATCCCGGTGGGCGACGGCGCCGCGCTGCTGCGGCGGCGCCCCGACGTGCGCGAGGCCGAGCGC
CAGCTGGCGGCGGCCACCGCGCGGATCGGCGTGGCGATCGGCGCGATGTATCCGACCGTGAGTCTCGGCGCCTCGGCGGG
CACCACCGGCATCTTCGGCGATCTGCTCTCGTCGCGGACCAACCGCTGGGCGCTCGGCCCGCTGATCAGCTGGACCTTCC
CGGCCAACGGCCAGCGGGCCCGCGTGCGCGAGGCCGAGGCCGCGACGGGCGGCGCGCTCGCGCACTTCGACGGCGTGGTG
CTGAACGCGCTGCGCGAGACGCAGACGAGCCTGGCGACCTACGCGGCCGACACGCAGCGCGCCGACGCGCTGCGCACCGC
CTACCAGTCGGCGCGCGATGCGGCCGAGGAAACGCACCGGCTCTACGCGGCGGGCCGCGAATCGTTCATCTCGGATCTCG
ATGCGACGCGCACGCTGACGAGCGTGCAGGCGCGGGTCGCCGCCGTGCAAGCCCAGGTCGCGGCCGATCAGGTGCGCCTG
TTCCTTGCGCTGGGCGGGGGCTGGGAGGAGCCGGCCGCCGCCGCGGCGGAGCCGGCCGGCGCGATGCCGCCGGCGGCGGG
GCCTGCACCGGCCGGGACCGGCGCGCGCTGA

Upstream 100 bases:

>100_bases
GCGCCTCCGGCCCGGCGCGCGTCGCAGGCACGGCCAGCGCCGCCCGGCCGACCGGCGCCTCGCCACCGGCATCGCAGGCG
GCCACGGCCGGCGCCGCGCC

Downstream 100 bases:

>100_bases
ACCGGCGGCACGGCGTCGAACCGTGCCGCCGCGCCGGGCCGCGCCGGGCCGCGCCGCGCGATGCCGCCAACCCGCGCCGC
GGGCTCGCACGCCCGCCTCG

Product: NodT family RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTRRFAPAAGRPPASPSRRAAAMAALSAALVLAACTTVGPDYRLPKQALVNAPLAGAPLDGADDGRALVSHEAVPARWWR
LYDDPVLDALVQQALDSNTDLRVAAANLARARAALEVESAQGGLTGNLEGGAQRAQESAEQYLLTDKLPVVNEGAFNFNV
SYEFDLFGKLRRGVEAARADREAVKAAGDLARITVVAEVVRAYVEACSAGEELAIAKRSLDLQRQRVALSRRLRDAGRGS
QPEVTRGVAQVQTLAADIPRFEGRRRVAQYQLAALLARSPAELPAAAHGCRQLPSLKQAIPVGDGAALLRRRPDVREAER
QLAAATARIGVAIGAMYPTVSLGASAGTTGIFGDLLSSRTNRWALGPLISWTFPANGQRARVREAEAATGGALAHFDGVV
LNALRETQTSLATYAADTQRADALRTAYQSARDAAEETHRLYAAGRESFISDLDATRTLTSVQARVAAVQAQVAADQVRL
FLALGGGWEEPAAAAAEPAGAMPPAAGPAPAGTGAR

Sequences:

>Translated_516_residues
MTRRFAPAAGRPPASPSRRAAAMAALSAALVLAACTTVGPDYRLPKQALVNAPLAGAPLDGADDGRALVSHEAVPARWWR
LYDDPVLDALVQQALDSNTDLRVAAANLARARAALEVESAQGGLTGNLEGGAQRAQESAEQYLLTDKLPVVNEGAFNFNV
SYEFDLFGKLRRGVEAARADREAVKAAGDLARITVVAEVVRAYVEACSAGEELAIAKRSLDLQRQRVALSRRLRDAGRGS
QPEVTRGVAQVQTLAADIPRFEGRRRVAQYQLAALLARSPAELPAAAHGCRQLPSLKQAIPVGDGAALLRRRPDVREAER
QLAAATARIGVAIGAMYPTVSLGASAGTTGIFGDLLSSRTNRWALGPLISWTFPANGQRARVREAEAATGGALAHFDGVV
LNALRETQTSLATYAADTQRADALRTAYQSARDAAEETHRLYAAGRESFISDLDATRTLTSVQARVAAVQAQVAADQVRL
FLALGGGWEEPAAAAAEPAGAMPPAAGPAPAGTGAR
>Mature_515_residues
TRRFAPAAGRPPASPSRRAAAMAALSAALVLAACTTVGPDYRLPKQALVNAPLAGAPLDGADDGRALVSHEAVPARWWRL
YDDPVLDALVQQALDSNTDLRVAAANLARARAALEVESAQGGLTGNLEGGAQRAQESAEQYLLTDKLPVVNEGAFNFNVS
YEFDLFGKLRRGVEAARADREAVKAAGDLARITVVAEVVRAYVEACSAGEELAIAKRSLDLQRQRVALSRRLRDAGRGSQ
PEVTRGVAQVQTLAADIPRFEGRRRVAQYQLAALLARSPAELPAAAHGCRQLPSLKQAIPVGDGAALLRRRPDVREAERQ
LAAATARIGVAIGAMYPTVSLGASAGTTGIFGDLLSSRTNRWALGPLISWTFPANGQRARVREAEAATGGALAHFDGVVL
NALRETQTSLATYAADTQRADALRTAYQSARDAAEETHRLYAAGRESFISDLDATRTLTSVQARVAAVQAQVAADQVRLF
LALGGGWEEPAAAAAEPAGAMPPAAGPAPAGTGAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=477, Percent_Identity=25.9958071278826, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54148; Mature: 54016

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRFAPAAGRPPASPSRRAAAMAALSAALVLAACTTVGPDYRLPKQALVNAPLAGAPLD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCC
GADDGRALVSHEAVPARWWRLYDDPVLDALVQQALDSNTDLRVAAANLARARAALEVESA
CCCCCCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHEECCC
QGGLTGNLEGGAQRAQESAEQYLLTDKLPVVNEGAFNFNVSYEFDLFGKLRRGVEAARAD
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEEHHHHHHHHHHHHHHHHH
REAVKAAGDLARITVVAEVVRAYVEACSAGEELAIAKRSLDLQRQRVALSRRLRDAGRGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QPEVTRGVAQVQTLAADIPRFEGRRRVAQYQLAALLARSPAELPAAAHGCRQLPSLKQAI
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHC
PVGDGAALLRRRPDVREAERQLAAATARIGVAIGAMYPTVSLGASAGTTGIFGDLLSSRT
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCC
NRWALGPLISWTFPANGQRARVREAEAATGGALAHFDGVVLNALRETQTSLATYAADTQR
CCEEECCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADALRTAYQSARDAAEETHRLYAAGRESFISDLDATRTLTSVQARVAAVQAQVAADQVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
FLALGGGWEEPAAAAAEPAGAMPPAAGPAPAGTGAR
EEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRRFAPAAGRPPASPSRRAAAMAALSAALVLAACTTVGPDYRLPKQALVNAPLAGAPLD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCC
GADDGRALVSHEAVPARWWRLYDDPVLDALVQQALDSNTDLRVAAANLARARAALEVESA
CCCCCCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHEECCC
QGGLTGNLEGGAQRAQESAEQYLLTDKLPVVNEGAFNFNVSYEFDLFGKLRRGVEAARAD
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEEHHHHHHHHHHHHHHHHH
REAVKAAGDLARITVVAEVVRAYVEACSAGEELAIAKRSLDLQRQRVALSRRLRDAGRGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QPEVTRGVAQVQTLAADIPRFEGRRRVAQYQLAALLARSPAELPAAAHGCRQLPSLKQAI
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHC
PVGDGAALLRRRPDVREAERQLAAATARIGVAIGAMYPTVSLGASAGTTGIFGDLLSSRT
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCC
NRWALGPLISWTFPANGQRARVREAEAATGGALAHFDGVVLNALRETQTSLATYAADTQR
CCEEECCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADALRTAYQSARDAAEETHRLYAAGRESFISDLDATRTLTSVQARVAAVQAQVAADQVRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
FLALGGGWEEPAAAAAEPAGAMPPAAGPAPAGTGAR
EEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]