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The map label for this gene is alkK [H]

Identifier: 238024592

GI number: 238024592

Start: 1539159

End: 1540832

Strand: Reverse

Name: alkK [H]

Synonym: bglu_2g12030

Alternate gene names: 238024592

Gene position: 1540832-1539159 (Counterclockwise)

Preceding gene: 238024593

Following gene: 238024590

Centisome position: 54.5

GC content: 71.74

Gene sequence:

>1674_bases
ATGACGCAGCTGTTCGAGACCGGGCTGGGCCGGCGCGAGGCGAACCACGTAGCGCTCACGCCGATCGACTTCATCGCGCG
CGCGGCCGAGGTGTACGGCAGCCGGCTCGCGGTGGTGCATGGCGCGCAGCGCTACACCTGGAGCGAGGCCTACGAGCGCG
CGCGGCGGCTGGCCGGGGCGCTCGCGCAGGCCGGCATCGGCCGCGGCGACACGGTGGCGGTCGTGCTGCCCAACATCCCG
CCGATGATCGACGCGCACTTCGGCGTGCCGATGGCAGGCGCCGTGCTCAACGCCATCAACACGCGGCTCGATGCGGCCTC
GATCCTGTTCATGCTGCGCCATGGCGAGGCCAAGCTGCTGATCGTCGATACCGAGTACGCCGAACTCGCGCGCCGCGTGG
CGCAGGCGCTGCCCGGGCTGCGCATCGTCTGCGTGGCCGACGCGCTGCCGGCCGACCCCGCCAGCTTCGGCGGCGCGACC
GACTACGAGGCGTTCCTCGCCAGCGGCGACCCCGCCTACGCCTGGGCGCCGCCCGCCGACGAGTGGGACGCGATCGCGCT
CAACTACACCTCGGGCACCACCGGCGAGCCGAAGGGCGTGGTCTACCACCATCGCGGCGCCTATCTCGCGGCGCTCTCCA
ACCTGCTCGAATGGGACATGCCCAAGCATGCCGTCTACCTCTGGACGCTGCCGATGTTCCATTGCAACGGCTGGTGCTTC
CCGTGGGCGGTGGCGGCGCGCGCGGGCGTGAACGTCTGCCTGCGCAAATTCGATCCGCGCAGCGTGTTCGAGCTGATCCG
CCGCGAGCGCGTCACCCACTACAGCGGCGCGCCGATCGTGCACGGCGCGCTGGCCGACGCGCCGGCCGAGTGGCGCGCCG
GCATCGACCACCTGGTGCATGCCCAGGTGGCGGGCGCCGCGCCCGCGCCGGCCGTGATCGCGAAAATGAAGGCGATCGGC
TTCGAGCTGCTGCACGTGTACGGCCTGACCGAGATCTACGGGCCGGCCACCGTCTGCGCCGAGCAGCAGCACTGGGCCGC
GCTGCCCGACGACGAGGCGGCGGGCTTGAAGGCGCGCCAGGGCGTGCGCTACCACCTGCAGGCGGGCGCCACCGTGCTCG
ATCCCGACACCATGCGGAGCGTGCCGGCCGATGGCGAGACGCTCGGCGAGATCATGTTCCGCGGCAACCTCTGCATGAAG
GGCTACCTGAAGAATCCGGCCGCCACCGACGAGGCGTTCGCGGGCGGCTGGTTCCATACCGGCGATCTCGGCGTGCTGAC
GCCGGACGGCTACATCCGCATCAAGGACCGTCGCAAGGACATCATCATCTCGGGCGGCGAGAACATCTCGAGCATCGAGC
TGGAGGACGCGCTGTACCGGCATCCGGCCGTGGCGGTGGCGGCGGTGGTGGCGCTGCCGGACCCGAAGTGGGGCGAGGTG
CCGTGCGCGTTCGTCGAGTTGCGCGAGGGCATGGCGGCCAGCGAGGCCGAGATCCTCGCCCATTGCCGCACGCTGCTGGC
CGGCTACAAGATGCCCAAGGCGGTCCGCTTCGGCGAGCTGCCGAAGACGTCCACCGGCAAGATCCAGAAATTCCAGCTGC
GCAGCCGGGTACGCGCCGAGCGTGAGGCCGGCGCGGGCGCGCAGGCGGGGGCGGCCGCGGACCGGACGGGGTGA

Upstream 100 bases:

>100_bases
CTGTCGCAATTCGGGCGGCCTCGCCATCGCGAACAACGAGCGGGCGCGGCCTGCGCGAGCGGCCCGCGCCCGACGCCAAC
GAGCAACACGGAGACACGAC

Downstream 100 bases:

>100_bases
GGCGCGCCAGCGCCGGGCGCCATTCAGCCCGGGTTCCGGTGCGGACGAGGCCGAGTGCCGCCCGGGTTCAGGCGGCCGGC
CGGGCGGTGGCGAGCCGGCC

Product: acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MTQLFETGLGRREANHVALTPIDFIARAAEVYGSRLAVVHGAQRYTWSEAYERARRLAGALAQAGIGRGDTVAVVLPNIP
PMIDAHFGVPMAGAVLNAINTRLDAASILFMLRHGEAKLLIVDTEYAELARRVAQALPGLRIVCVADALPADPASFGGAT
DYEAFLASGDPAYAWAPPADEWDAIALNYTSGTTGEPKGVVYHHRGAYLAALSNLLEWDMPKHAVYLWTLPMFHCNGWCF
PWAVAARAGVNVCLRKFDPRSVFELIRRERVTHYSGAPIVHGALADAPAEWRAGIDHLVHAQVAGAAPAPAVIAKMKAIG
FELLHVYGLTEIYGPATVCAEQQHWAALPDDEAAGLKARQGVRYHLQAGATVLDPDTMRSVPADGETLGEIMFRGNLCMK
GYLKNPAATDEAFAGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIELEDALYRHPAVAVAAVVALPDPKWGEV
PCAFVELREGMAASEAEILAHCRTLLAGYKMPKAVRFGELPKTSTGKIQKFQLRSRVRAEREAGAGAQAGAAADRTG

Sequences:

>Translated_557_residues
MTQLFETGLGRREANHVALTPIDFIARAAEVYGSRLAVVHGAQRYTWSEAYERARRLAGALAQAGIGRGDTVAVVLPNIP
PMIDAHFGVPMAGAVLNAINTRLDAASILFMLRHGEAKLLIVDTEYAELARRVAQALPGLRIVCVADALPADPASFGGAT
DYEAFLASGDPAYAWAPPADEWDAIALNYTSGTTGEPKGVVYHHRGAYLAALSNLLEWDMPKHAVYLWTLPMFHCNGWCF
PWAVAARAGVNVCLRKFDPRSVFELIRRERVTHYSGAPIVHGALADAPAEWRAGIDHLVHAQVAGAAPAPAVIAKMKAIG
FELLHVYGLTEIYGPATVCAEQQHWAALPDDEAAGLKARQGVRYHLQAGATVLDPDTMRSVPADGETLGEIMFRGNLCMK
GYLKNPAATDEAFAGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIELEDALYRHPAVAVAAVVALPDPKWGEV
PCAFVELREGMAASEAEILAHCRTLLAGYKMPKAVRFGELPKTSTGKIQKFQLRSRVRAEREAGAGAQAGAAADRTG
>Mature_556_residues
TQLFETGLGRREANHVALTPIDFIARAAEVYGSRLAVVHGAQRYTWSEAYERARRLAGALAQAGIGRGDTVAVVLPNIPP
MIDAHFGVPMAGAVLNAINTRLDAASILFMLRHGEAKLLIVDTEYAELARRVAQALPGLRIVCVADALPADPASFGGATD
YEAFLASGDPAYAWAPPADEWDAIALNYTSGTTGEPKGVVYHHRGAYLAALSNLLEWDMPKHAVYLWTLPMFHCNGWCFP
WAVAARAGVNVCLRKFDPRSVFELIRRERVTHYSGAPIVHGALADAPAEWRAGIDHLVHAQVAGAAPAPAVIAKMKAIGF
ELLHVYGLTEIYGPATVCAEQQHWAALPDDEAAGLKARQGVRYHLQAGATVLDPDTMRSVPADGETLGEIMFRGNLCMKG
YLKNPAATDEAFAGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIELEDALYRHPAVAVAAVVALPDPKWGEVP
CAFVELREGMAASEAEILAHCRTLLAGYKMPKAVRFGELPKTSTGKIQKFQLRSRVRAEREAGAGAQAGAAADRTG

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=546, Percent_Identity=28.021978021978, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI187761345, Length=545, Percent_Identity=28.8073394495413, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI187761343, Length=545, Percent_Identity=28.8073394495413, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI58082049, Length=513, Percent_Identity=28.6549707602339, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI157311624, Length=513, Percent_Identity=28.0701754385965, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI157311622, Length=513, Percent_Identity=28.0701754385965, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI42544132, Length=514, Percent_Identity=27.0428015564202, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=27.906976744186, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=25.7309941520468, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI38505220, Length=514, Percent_Identity=27.0428015564202, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI27477105, Length=500, Percent_Identity=25, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI28416953, Length=540, Percent_Identity=24.8148148148148, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI40807491, Length=497, Percent_Identity=26.9617706237425, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI42794756, Length=484, Percent_Identity=25, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI42794760, Length=484, Percent_Identity=25, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI42794758, Length=484, Percent_Identity=25, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI57165410, Length=507, Percent_Identity=24.8520710059172, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI57165412, Length=507, Percent_Identity=24.8520710059172, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI13236579, Length=487, Percent_Identity=25.4620123203285, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI13375727, Length=182, Percent_Identity=31.8681318681319, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=29.6367112810707, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788107, Length=557, Percent_Identity=26.5709156193896, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI145693145, Length=537, Percent_Identity=29.9813780260708, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1786810, Length=524, Percent_Identity=28.0534351145038, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788595, Length=521, Percent_Identity=24.9520153550864, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=529, Percent_Identity=26.2759924385633, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71994690, Length=529, Percent_Identity=26.2759924385633, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71994694, Length=529, Percent_Identity=26.2759924385633, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=27.7985074626866, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17559526, Length=512, Percent_Identity=27.734375, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17558820, Length=512, Percent_Identity=27.5390625, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17538037, Length=536, Percent_Identity=24.4402985074627, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=24.3494423791822, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=24.4186046511628, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560140, Length=526, Percent_Identity=24.1444866920152, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=494, Percent_Identity=24.4939271255061, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=543, Percent_Identity=25.414364640884, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=593, Percent_Identity=22.4283305227656, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=580, Percent_Identity=23.2758620689655, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=580, Percent_Identity=23.2758620689655, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=373, Percent_Identity=28.686327077748, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=178, Percent_Identity=30.3370786516854, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24581924, Length=550, Percent_Identity=28.7272727272727, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355181, Length=533, Percent_Identity=24.953095684803, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI18859661, Length=521, Percent_Identity=27.2552783109405, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550496, Length=550, Percent_Identity=24.1818181818182, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45552801, Length=550, Percent_Identity=24.1818181818182, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583465, Length=553, Percent_Identity=24.9547920433996, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24583463, Length=553, Percent_Identity=24.9547920433996, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364798, Length=553, Percent_Identity=24.9547920433996, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648260, Length=521, Percent_Identity=23.9923224568138, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653035, Length=561, Percent_Identity=22.4598930481283, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358303, Length=474, Percent_Identity=22.3628691983122, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356947, Length=552, Percent_Identity=21.195652173913, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161076582, Length=571, Percent_Identity=21.5411558669002, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI20130357, Length=501, Percent_Identity=21.9560878243513, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=22.2879684418146, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648255, Length=507, Percent_Identity=22.2879684418146, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356441, Length=512, Percent_Identity=21.2890625, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59921; Mature: 59790

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLFETGLGRREANHVALTPIDFIARAAEVYGSRLAVVHGAQRYTWSEAYERARRLAGA
CCCHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHH
LAQAGIGRGDTVAVVLPNIPPMIDAHFGVPMAGAVLNAINTRLDAASILFMLRHGEAKLL
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
IVDTEYAELARRVAQALPGLRIVCVADALPADPASFGGATDYEAFLASGDPAYAWAPPAD
EEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCEEECCCCC
EWDAIALNYTSGTTGEPKGVVYHHRGAYLAALSNLLEWDMPKHAVYLWTLPMFHCNGWCF
CCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCH
PWAVAARAGVNVCLRKFDPRSVFELIRRERVTHYSGAPIVHGALADAPAEWRAGIDHLVH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEECHHHCCCHHHHHHHHHHHH
AQVAGAAPAPAVIAKMKAIGFELLHVYGLTEIYGPATVCAEQQHWAALPDDEAAGLKARQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHC
GVRYHLQAGATVLDPDTMRSVPADGETLGEIMFRGNLCMKGYLKNPAATDEAFAGGWFHT
CCEEEEECCCEEECCHHHHCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE
GDLGVLTPDGYIRIKDRRKDIIISGGENISSIELEDALYRHPAVAVAAVVALPDPKWGEV
CCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHEEEECCCCCCCCC
PCAFVELREGMAASEAEILAHCRTLLAGYKMPKAVRFGELPKTSTGKIQKFQLRSRVRAE
CHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
REAGAGAQAGAAADRTG
HHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQLFETGLGRREANHVALTPIDFIARAAEVYGSRLAVVHGAQRYTWSEAYERARRLAGA
CCHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHH
LAQAGIGRGDTVAVVLPNIPPMIDAHFGVPMAGAVLNAINTRLDAASILFMLRHGEAKLL
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
IVDTEYAELARRVAQALPGLRIVCVADALPADPASFGGATDYEAFLASGDPAYAWAPPAD
EEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCEEECCCCC
EWDAIALNYTSGTTGEPKGVVYHHRGAYLAALSNLLEWDMPKHAVYLWTLPMFHCNGWCF
CCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCH
PWAVAARAGVNVCLRKFDPRSVFELIRRERVTHYSGAPIVHGALADAPAEWRAGIDHLVH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEECHHHCCCHHHHHHHHHHHH
AQVAGAAPAPAVIAKMKAIGFELLHVYGLTEIYGPATVCAEQQHWAALPDDEAAGLKARQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHC
GVRYHLQAGATVLDPDTMRSVPADGETLGEIMFRGNLCMKGYLKNPAATDEAFAGGWFHT
CCEEEEECCCEEECCHHHHCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE
GDLGVLTPDGYIRIKDRRKDIIISGGENISSIELEDALYRHPAVAVAAVVALPDPKWGEV
CCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHEEEECCCCCCCCC
PCAFVELREGMAASEAEILAHCRTLLAGYKMPKAVRFGELPKTSTGKIQKFQLRSRVRAE
CHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
REAGAGAQAGAAADRTG
HHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]