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The map label for this gene is polA [H]

Identifier: 229917676

GI number: 229917676

Start: 1912573

End: 1913565

Strand: Reverse

Name: polA [H]

Synonym: EAT1b_1953

Alternate gene names: 229917676

Gene position: 1913565-1912573 (Counterclockwise)

Preceding gene: 229917677

Following gene: 229917674

Centisome position: 63.79

GC content: 49.95

Gene sequence:

>993_bases
ATGGAACGCAAACTATTTTTGATTGACGGTAACTCCATGCTGGCCAGTGCTTATTATGGTGCCGCCGCGAGCCGAACGAA
AAAAGGACGCGAGGTATCCAAAAACGATCGGAGTGCCGTCATCGGAATGACCGGCCTCATCCGGACGATTCTACGCAGAC
ATCGTCCGACTCATTTTGTCGTCGTCTGGGACGTCTCACGTGAAACATTATGGCGTCGAGAACTGTACCCGGGCTACAAA
CGTCGCAGACGTCAAACACCGCCTGAATTGAAAGCGCAGATGGAACTCATGCGCCAACTGCTTGAGGATAGTGGAATCCC
ACAATTCCAAGTAGAAGGGTATGAAGGGGAAGACTTGATTGCCCACATCGCCCGTAAATTCAGTTGCAGTGCCCCTGTCG
TCATCATGACAAAAGACAAAGACTTGTTACAGCTTGTCAGTCCACGAATCACCGTTTGGTTACAAACACAGGAGCTTCAG
CAAATGCAGGCTCGCTGTGATATTCGTGATAATCGTCCGTTGCCGCTCAAGCGTCATTTAGAAATTCGACCAGAAGAGAT
TGCAGCGTTATACGAAGGATTGAAACCGAAGCAACTGTCAGCCGTCAAAGCATTGACAGGAGACCGTTCTGACGGAATCC
CTCCAGTTGCCGGGCTCGACGAAAGCCAATCGGTTCAACTCATCAAACGATTCGGTTCGCTCGATAAGCTGTATTCTGCC
TTGAGCATCAAAGGTGAAAAACGACAACAAGTAAATGAAACGATCGCGGCACTGACGTCAGAGGAGGTGCCCCGTAACTT
GAGACGAACACGCAAAAAAGCAGAGCGCAACATGGAGCTCGTTCGACTCGATGTCACGGTGCCGGCACTTGAACATTTGA
AGTTGGCTCATCTCGAGCTCAGCCTCAACCCAAAACGAGTCGAGCAAGCGTATCAAAAACGCGGACTTCGTCTCTCGTTT
AACGCAAGTCAGAAGAAAACATCCGCACGTTAA

Upstream 100 bases:

>100_bases
TATGTTGCTTATTTGAGTGTCACCCACTCAATGACACAACATCACCGAAACATGGCGAGATGTTCGGTTTAAACAGAAAG
AAATAGAAAGAAGGTTACGC

Downstream 100 bases:

>100_bases
AGAACGGACGCCATCAAGCGTCCGTTCTTTTAATATGCACGGTAATCCCGTCTTCATCAGTCAACGTCCATTCAGACGAC
GTTCCGCCTAACGCTCGGTT

Product: 5'-3' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MERKLFLIDGNSMLASAYYGAAASRTKKGREVSKNDRSAVIGMTGLIRTILRRHRPTHFVVVWDVSRETLWRRELYPGYK
RRRRQTPPELKAQMELMRQLLEDSGIPQFQVEGYEGEDLIAHIARKFSCSAPVVIMTKDKDLLQLVSPRITVWLQTQELQ
QMQARCDIRDNRPLPLKRHLEIRPEEIAALYEGLKPKQLSAVKALTGDRSDGIPPVAGLDESQSVQLIKRFGSLDKLYSA
LSIKGEKRQQVNETIAALTSEEVPRNLRRTRKKAERNMELVRLDVTVPALEHLKLAHLELSLNPKRVEQAYQKRGLRLSF
NASQKKTSAR

Sequences:

>Translated_330_residues
MERKLFLIDGNSMLASAYYGAAASRTKKGREVSKNDRSAVIGMTGLIRTILRRHRPTHFVVVWDVSRETLWRRELYPGYK
RRRRQTPPELKAQMELMRQLLEDSGIPQFQVEGYEGEDLIAHIARKFSCSAPVVIMTKDKDLLQLVSPRITVWLQTQELQ
QMQARCDIRDNRPLPLKRHLEIRPEEIAALYEGLKPKQLSAVKALTGDRSDGIPPVAGLDESQSVQLIKRFGSLDKLYSA
LSIKGEKRQQVNETIAALTSEEVPRNLRRTRKKAERNMELVRLDVTVPALEHLKLAHLELSLNPKRVEQAYQKRGLRLSF
NASQKKTSAR
>Mature_330_residues
MERKLFLIDGNSMLASAYYGAAASRTKKGREVSKNDRSAVIGMTGLIRTILRRHRPTHFVVVWDVSRETLWRRELYPGYK
RRRRQTPPELKAQMELMRQLLEDSGIPQFQVEGYEGEDLIAHIARKFSCSAPVVIMTKDKDLLQLVSPRITVWLQTQELQ
QMQARCDIRDNRPLPLKRHLEIRPEEIAALYEGLKPKQLSAVKALTGDRSDGIPPVAGLDESQSVQLIKRFGSLDKLYSA
LSIKGEKRQQVNETIAALTSEEVPRNLRRTRKKAERNMELVRLDVTVPALEHLKLAHLELSLNPKRVEQAYQKRGLRLSF
NASQKKTSAR

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 5' to 3' exonuclease activity [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=303, Percent_Identity=30.03300330033, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI226510970, Length=209, Percent_Identity=30.1435406698565, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37838; Mature: 37838

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKLFLIDGNSMLASAYYGAAASRTKKGREVSKNDRSAVIGMTGLIRTILRRHRPTHFV
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEE
VVWDVSRETLWRRELYPGYKRRRRQTPPELKAQMELMRQLLEDSGIPQFQVEGYEGEDLI
EEEECCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
AHIARKFSCSAPVVIMTKDKDLLQLVSPRITVWLQTQELQQMQARCDIRDNRPLPLKRHL
HHHHHHHCCCCCEEEEECCHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHCC
EIRPEEIAALYEGLKPKQLSAVKALTGDRSDGIPPVAGLDESQSVQLIKRFGSLDKLYSA
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHH
LSIKGEKRQQVNETIAALTSEEVPRNLRRTRKKAERNMELVRLDVTVPALEHLKLAHLEL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHEEEEEEE
SLNPKRVEQAYQKRGLRLSFNASQKKTSAR
CCCHHHHHHHHHHCCCEEEECCCCHHCCCC
>Mature Secondary Structure
MERKLFLIDGNSMLASAYYGAAASRTKKGREVSKNDRSAVIGMTGLIRTILRRHRPTHFV
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEE
VVWDVSRETLWRRELYPGYKRRRRQTPPELKAQMELMRQLLEDSGIPQFQVEGYEGEDLI
EEEECCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
AHIARKFSCSAPVVIMTKDKDLLQLVSPRITVWLQTQELQQMQARCDIRDNRPLPLKRHL
HHHHHHHCCCCCEEEEECCHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHCC
EIRPEEIAALYEGLKPKQLSAVKALTGDRSDGIPPVAGLDESQSVQLIKRFGSLDKLYSA
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHH
LSIKGEKRQQVNETIAALTSEEVPRNLRRTRKKAERNMELVRLDVTVPALEHLKLAHLEL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHEEEEEEE
SLNPKRVEQAYQKRGLRLSFNASQKKTSAR
CCCHHHHHHHHHHCCCEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7557480; 9016716; 9440698 [H]