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The map label for this gene is irgB [H]

Identifier: 227080684

GI number: 227080684

Start: 488530

End: 489426

Strand: Reverse

Name: irgB [H]

Synonym: VCM66_0459

Alternate gene names: 227080684

Gene position: 489426-488530 (Counterclockwise)

Preceding gene: 227080691

Following gene: 227080682

Centisome position: 16.92

GC content: 50.95

Gene sequence:

>897_bases
ATGCAAGATCTCAGCGCCGTAAAAGCTTTCCATGCGCTTTGCCAACATAAGAGCCTGACTGCCGCTGCCAAAGCGCTTGA
ACAGCCTAAATCCACCCTGAGTCGCCGTTTGGCGCAACTTGAAGAGGACTTGGGACAAAGCTTGTTGATGCGTCAAGGCA
ACCGCTTAACGCTCACCAAAGCAGGAGAAGTGTTTGCGGTTTACTCGGAGCAGCTACTTGAACTGGCCAATAAAAGTCAG
GAAGCGTTGCAAGAATTGAACAATCAAGTCACTGGCGAACTCACGCTGGTGGTACACCCCAATTTGATCCGCGGCTGGCT
CAGCCAAGTACTGGATGAGTTTATGCAGCAGCATTCGACATTGAAGATCCGTCTACTCAGCCAGTTTCAACACAGTGATG
AAGTGTTTGAGCCCGATTTGATCATTTGGATTGAACACGCCGCCCCCATGGGTTATCGCAAAGAACGCTTAGGCTATTGG
CGCTATGCCGCTTACGCCTCTCCCAAATATTTGGCCCATCGAGATAAACCGACCCATCCTCGTGAGCTGATTCATCACCC
ATGGATTGATTTTATTGCTTGTCGGCGCGCGGAGCTTGAACTGCACCATCCAGAGTTCGGCAGTTATTCACTGCCAGCAC
TAGAGAGTCGTTTACAAAGCGATAATCTTGCCATGCAAGCCGATGCGATTGCTAAAGGTCGTGGTATTGGTTTGCTTCCC
ACTTGGTTTGCCAATGGTTTTGAAACGGCGCATCCGGGCAGCCTCATTCCTTGCGTCAACGGATGGCAATCACAGCCAAC
AGAAATCAACTGCTTCTATCCGCTCGGTCGTCACCCACTTCGGCTGCGCCTATTTATTGATGCGCTCCGCCAAGCAAGGC
CCGATGAGTGGCAATAA

Upstream 100 bases:

>100_bases
ATTATTCATATATTCTGTACTGGAGATAGATCGACACATGGAATAGTTCGGTCCAAATATGGAACGTCTGTGGTAATTAT
TCTTTAAGGGTCAAATACCT

Downstream 100 bases:

>100_bases
AAAATCCCGCCGCAGCGGGATTTTTTATCATCGATTTTTCTTTGCGTAACAAAGAAATAAATCGCTTTTAATTACGCCAG
CAGTTCTTTAGCTTGCTTCA

Product: iron-regulated virulence regulatory protein IrgB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MQDLSAVKAFHALCQHKSLTAAAKALEQPKSTLSRRLAQLEEDLGQSLLMRQGNRLTLTKAGEVFAVYSEQLLELANKSQ
EALQELNNQVTGELTLVVHPNLIRGWLSQVLDEFMQQHSTLKIRLLSQFQHSDEVFEPDLIIWIEHAAPMGYRKERLGYW
RYAAYASPKYLAHRDKPTHPRELIHHPWIDFIACRRAELELHHPEFGSYSLPALESRLQSDNLAMQADAIAKGRGIGLLP
TWFANGFETAHPGSLIPCVNGWQSQPTEINCFYPLGRHPLRLRLFIDALRQARPDEWQ

Sequences:

>Translated_298_residues
MQDLSAVKAFHALCQHKSLTAAAKALEQPKSTLSRRLAQLEEDLGQSLLMRQGNRLTLTKAGEVFAVYSEQLLELANKSQ
EALQELNNQVTGELTLVVHPNLIRGWLSQVLDEFMQQHSTLKIRLLSQFQHSDEVFEPDLIIWIEHAAPMGYRKERLGYW
RYAAYASPKYLAHRDKPTHPRELIHHPWIDFIACRRAELELHHPEFGSYSLPALESRLQSDNLAMQADAIAKGRGIGLLP
TWFANGFETAHPGSLIPCVNGWQSQPTEINCFYPLGRHPLRLRLFIDALRQARPDEWQ
>Mature_298_residues
MQDLSAVKAFHALCQHKSLTAAAKALEQPKSTLSRRLAQLEEDLGQSLLMRQGNRLTLTKAGEVFAVYSEQLLELANKSQ
EALQELNNQVTGELTLVVHPNLIRGWLSQVLDEFMQQHSTLKIRLLSQFQHSDEVFEPDLIIWIEHAAPMGYRKERLGYW
RYAAYASPKYLAHRDKPTHPRELIHHPWIDFIACRRAELELHHPEFGSYSLPALESRLQSDNLAMQADAIAKGRGIGLLP
TWFANGFETAHPGSLIPCVNGWQSQPTEINCFYPLGRHPLRLRLFIDALRQARPDEWQ

Specific function: Transcription activation of the irgA gene. In the presence of sufficient iron, transcription of both irgA and irgB is negatively regulated by a fur-like protein. In low iron conditions, negative regulation of transcription is removed, and production of ir

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=23.6301369863014, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=23.3676975945017, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787601, Length=120, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=125, Percent_Identity=32, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI145693105, Length=126, Percent_Identity=30.1587301587302, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=28.6713286713287, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787589, Length=305, Percent_Identity=23.2786885245902, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788706, Length=124, Percent_Identity=31.4516129032258, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34069; Mature: 34069

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLSAVKAFHALCQHKSLTAAAKALEQPKSTLSRRLAQLEEDLGQSLLMRQGNRLTLTK
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AGEVFAVYSEQLLELANKSQEALQELNNQVTGELTLVVHPNLIRGWLSQVLDEFMQQHST
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
LKIRLLSQFQHSDEVFEPDLIIWIEHAAPMGYRKERLGYWRYAAYASPKYLAHRDKPTHP
EEHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHCCCHHHHCCCCCCCH
RELIHHPWIDFIACRRAELELHHPEFGSYSLPALESRLQSDNLAMQADAIAKGRGIGLLP
HHHHHCCHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHCCCCCEEHHHHHHCCCCCCCCH
TWFANGFETAHPGSLIPCVNGWQSQPTEINCFYPLGRHPLRLRLFIDALRQARPDEWQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQDLSAVKAFHALCQHKSLTAAAKALEQPKSTLSRRLAQLEEDLGQSLLMRQGNRLTLTK
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AGEVFAVYSEQLLELANKSQEALQELNNQVTGELTLVVHPNLIRGWLSQVLDEFMQQHST
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
LKIRLLSQFQHSDEVFEPDLIIWIEHAAPMGYRKERLGYWRYAAYASPKYLAHRDKPTHP
EEHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHCCCHHHHCCCCCCCH
RELIHHPWIDFIACRRAELELHHPEFGSYSLPALESRLQSDNLAMQADAIAKGRGIGLLP
HHHHHCCHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHCCCCCEEHHHHHHCCCCCCCCH
TWFANGFETAHPGSLIPCVNGWQSQPTEINCFYPLGRHPLRLRLFIDALRQARPDEWQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1705025 [H]