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The map label for this gene is metK

Identifier: 227080682

GI number: 227080682

Start: 484939

End: 486096

Strand: Reverse

Name: metK

Synonym: VCM66_0457

Alternate gene names: 227080682

Gene position: 486096-484939 (Counterclockwise)

Preceding gene: 227080684

Following gene: 227080681

Centisome position: 16.81

GC content: 50.17

Gene sequence:

>1158_bases
ATGGCGATTAAGCACCTGTTTACTTCAGAATCGGTATCAGAAGGCCATCCAGATAAAATTGCAGACCAGATCTCTGATGC
AGTGTTGGACGCGATTTTTGAGCAAGATCCTAAAGCTCGCGTTGCTTGCGAAACTTACGTAAAAACTGGCATGGTGATGG
TGGGCGGTGAAATCACGACTTCCGCTTGGGTTGATATCGAAGAGATTACTCGTCAAACTGTTCGTGAAATTGGTTACGTG
CACTCAGACATGGGCTTCGATGCCAACTCTTGTGCGGTACTGAACACCATAGGTAAACAATCGCCAGACATCAACCAAGG
TGTTGATAAAGCCGATCCTAAAGAGCAAGGCGCGGGCGACCAAGGCATCATGTTTGGTTATGCAACCAACGAAACTGAAG
TGCTGATGCCAGCACCGATCACCTATGCGCATCGTCTGATGCAGCGCCAAGCTGAAGTTCGTAAAAATGGCACACTGCCA
TGGCTGCGCCCTGATGCGAAATCTCAGGTGACCTTCCAGTATGACCAAGGCAAGATCGTAGGTATTGATGCTGTAGTTCT
GTCGACTCAACACAGTGATTCAATCTCTACCGCAGATCTGCGTGAAGCGGTAATGGAAGAGATCATCAAGCCAGTTCTGC
CAGCAGAATGGTTGAGCAAAGAGACCAAATACTTCATCAACCCAACCGGTCGTTTTGTGATTGGTGGCCCAATGGGTGAC
TGTGGTCTGACCGGTCGTAAGATCATCGTTGATACCTACGGCGGCGCAGCGCGTCACGGTGGTGGTGCATTCTCTGGTAA
GGATCCATCCAAGGTTGACCGTTCAGCGGCTTACGCAGCACGTTACGTTGCGAAAAACATCGTTGCGGCGGGTATGGCTG
ATCGTTGTGAAATCCAACTCTCTTATGCTATCGGTGTCGCGGATCCAACGTCGATCATGGTGGAAACTTTCGGTACTGAG
AAAGTGTCGCAAGAGATCATCATTGAAGCCGTTCGCCAGTTCTTCGATCTGCGTCCATACGGTCTGCAAGAGATGCTGAA
CCTGCTGCAACCTATCTACAAGAAAACCGCAGCTTACGGTCACTTCGGCCGCGAAGAGTTCCCTTGGGAAGCCACCGACA
AAGCCGCGCTACTGCGTGACTTCGCTGGTCTAAAATAA

Upstream 100 bases:

>100_bases
GTACTAAATTGATCGATAACGTCATTATCTCTAAGCGACTCGAACCATAGGTCGAAAAGAACTGAGACGTTAGCGAATAA
CCACAAATATTGGAGCTATC

Downstream 100 bases:

>100_bases
GCATTATTTCAACTCATCGCTGACAAACGGCGAGGATTGCAATAGAGTTCAAGGCCCTTTCTCATGAAAGGGTCTTTTTT
ATGAGTTCGATGATAGGGTA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MAIKHLFTSESVSEGHPDKIADQISDAVLDAIFEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEEITRQTVREIGYV
HSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMQRQAEVRKNGTLP
WLRPDAKSQVTFQYDQGKIVGIDAVVLSTQHSDSISTADLREAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGD
CGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTE
KVSQEIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAALLRDFAGLK

Sequences:

>Translated_385_residues
MAIKHLFTSESVSEGHPDKIADQISDAVLDAIFEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEEITRQTVREIGYV
HSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMQRQAEVRKNGTLP
WLRPDAKSQVTFQYDQGKIVGIDAVVLSTQHSDSISTADLREAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGD
CGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTE
KVSQEIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAALLRDFAGLK
>Mature_384_residues
AIKHLFTSESVSEGHPDKIADQISDAVLDAIFEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEEITRQTVREIGYVH
SDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMQRQAEVRKNGTLPW
LRPDAKSQVTFQYDQGKIVGIDAVVLSTQHSDSISTADLREAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEK
VSQEIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAALLRDFAGLK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=56.951871657754, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=56.6844919786096, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=382, Percent_Identity=88.4816753926702, Blast_Score=709, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=373, Percent_Identity=57.372654155496, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=55.4666666666667, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17538494, Length=373, Percent_Identity=56.8364611260054, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=55.2083333333333, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=373, Percent_Identity=51.2064343163539, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=54.2944785276074, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=51.9287833827893, Blast_Score=330, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=53.8461538461538, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=62.6506024096386, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=71.7391304347826, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=374, Percent_Identity=58.8235294117647, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=58.6666666666667, Blast_Score=419, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45552159, Length=370, Percent_Identity=56.4864864864865, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580529, Length=370, Percent_Identity=56.4864864864865, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580537, Length=370, Percent_Identity=56.4864864864865, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580533, Length=370, Percent_Identity=56.4864864864865, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580535, Length=370, Percent_Identity=56.4864864864865, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580531, Length=370, Percent_Identity=56.4864864864865, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580527, Length=370, Percent_Identity=55.9459459459459, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=370, Percent_Identity=55.9459459459459, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=372, Percent_Identity=53.494623655914, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=55.5555555555556, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_VIBC3 (A5F9H4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F9H4
- SMR:   A5F9H4
- STRING:   A5F9H4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0024
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   VCHO345073:VC0395_A0024-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42127; Mature: 41996

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKHLFTSESVSEGHPDKIADQISDAVLDAIFEQDPKARVACETYVKTGMVMVGGEITT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEECC
SAWVDIEEITRQTVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGD
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCC
QGIMFGYATNETEVLMPAPITYAHRLMQRQAEVRKNGTLPWLRPDAKSQVTFQYDQGKIV
CCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEE
GIDAVVLSTQHSDSISTADLREAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQL
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVADPTSIMVETFGTEKVSQEIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYG
EEEEECCCCHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
HFGREEFPWEATDKAALLRDFAGLK
CCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AIKHLFTSESVSEGHPDKIADQISDAVLDAIFEQDPKARVACETYVKTGMVMVGGEITT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEECC
SAWVDIEEITRQTVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGD
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCC
QGIMFGYATNETEVLMPAPITYAHRLMQRQAEVRKNGTLPWLRPDAKSQVTFQYDQGKIV
CCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEE
GIDAVVLSTQHSDSISTADLREAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQL
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVADPTSIMVETFGTEKVSQEIIIEAVRQFFDLRPYGLQEMLNLLQPIYKKTAAYG
EEEEECCCCHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
HFGREEFPWEATDKAALLRDFAGLK
CCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA