Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 227080490

GI number: 227080490

Start: 265614

End: 267206

Strand: Reverse

Name: purH [H]

Synonym: VCM66_0261

Alternate gene names: 227080490

Gene position: 267206-265614 (Counterclockwise)

Preceding gene: 227080496

Following gene: 227080489

Centisome position: 9.24

GC content: 53.17

Gene sequence:

>1593_bases
ATGAACAACGCTCGTCCTATTCACCGTGCGCTTCTCAGCGTATCAGATAAAACCGGCATTGTTGAGTTCGCAAAAGCGCT
TGCAGAGCGCGGCGTTGAACTTCTCTCCACCGGTGGCACCGCTCGTCTATTGGCTGAGCAAGGCCTGACGGTAACGGAAG
TCTCCGATTACACCGGTTTCCCAGAAATGATGGACGGACGCGTCAAAACCCTGCATCCGAAAGTGCATGGCGGTATTTTA
GGCCGTCGCGGCCAAGATGACGCGGTGATGAATACCCACGGCATTCAGCCAATCGATATGGTGGTGGTGAACCTCTATCC
TTTCGCCCAAACGGTAGCTAACCCAAATTGCACGCTGGCTGATGCGGTGGAAAACATCGATATCGGCGGGCCGACTATGG
TGCGTTCTGCTGCAAAAAACCATAAAGATGTGGCCATTGTGGTGAATGCGCACGATTACGACCGTGTGATCCGCGAAATG
GATGCCAACCACAACTCCCTGACGTTAGCGACGCGTTTTGATCTGGCGATTGCCGCTTTTGAACATACCGCGGCGTACGA
TGGCATGATTGCGAACTACTTCGGCACACTCGTACCTTCTTATGGTGATAACAAAGAAGGGGATGAAGAGAGCAAATTCC
CTCGCACGTTCAATGCGCAGTTCATCAAAAAGCAAGATATGCGTTATGGCGAAAACAGCCACCAAGCGGCCGCGTTTTAT
GTAGAAGCCAATCCACAAGAAGCCTCAGTCGCCACCGCGCGCCAAATTCAAGGTAAAGCCCTTTCTTACAACAACATTGC
CGATACCGATGCCGCACTAGAGTGCGTCAAAGAGTTCAGCGAGCCAGCCTGTGTGATTGTGAAACACGCGAACCCATGTG
GTGTAGCGCTGGGCGATGATCTTCTGCAAGCTTACAATCGCGCTTACCAAACCGACCCAACCTCGGCATTTGGCGGCATC
ATTGCCTTTAACCGCGAGCTGGATGGCGAAACCGCAAGAGCGATCATCGAGCGTCAGTTTGTGGAAGTGATCATTGCGCC
GAAAGTCTCGCAAGCCGCGATCGACATCGTCGCAGCAAAACAAAACGTACGTTTGCTGGAGTGTGGTGAATGGCAAGGCC
AAACCACAGGATTTGATTTGAAGCGCGTGAATGGTGGCTTATTGGTGCAAGATCGTGACCAAGGCATGGTGGCACAAGAT
GACCTACAAGTGGTTTCGACTCGTCAACCTAGCGACGCTGAGCTGAAAGATGCTCTGTTCTGCTGGAAAGTAGCGAAATA
CGTGAAATCCAACGCGATTGTGTACGCCAAAGGCGACATGACCATCGGCATCGGCGCGGGTCAAATGAGCCGTGTTTACT
CCGCGAAGATCGCCGGCATCAAAGCCGCCGATGAAGGCTTGGAAGTCGCGGGCAGCGTGATGGCATCGGATGCCTTCTTC
CCCTTCCGTGATGGGATTGATGCTGCGGCCGAAGCGGGCATTACCTGTGTTATCCAACCGGGTGGCTCAATGCGCGACCA
AGAGGTGATTGATGCCGCCAACGAACACGGTATGGCGATGATCTTCACCGGTATGCGTCACTTCCGTCATTAA

Upstream 100 bases:

>100_bases
AGCTCTGTTGTTTTTTGCGCGGTGAGGCTTTGCAGCGAGCTTCCCCAACACCGCACAGCAGATCACTTTCGGCAAATATC
TTTTAAAGGAAAATGGAAGC

Downstream 100 bases:

>100_bases
TTGGCATGATTGGTATGGGGTGCAACGCGCCCCATCTTGTTTAAGTAAGGATTTCACAATGCAAGTTCTCATCATCGGCT
CTGGCGGTCGCGAACACGCA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNNARPIHRALLSVSDKTGIVEFAKALAERGVELLSTGGTARLLAEQGLTVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAVMNTHGIQPIDMVVVNLYPFAQTVANPNCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNAHDYDRVIREM
DANHNSLTLATRFDLAIAAFEHTAAYDGMIANYFGTLVPSYGDNKEGDEESKFPRTFNAQFIKKQDMRYGENSHQAAAFY
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGDDLLQAYNRAYQTDPTSAFGGI
IAFNRELDGETARAIIERQFVEVIIAPKVSQAAIDIVAAKQNVRLLECGEWQGQTTGFDLKRVNGGLLVQDRDQGMVAQD
DLQVVSTRQPSDAELKDALFCWKVAKYVKSNAIVYAKGDMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSVMASDAFF
PFRDGIDAAAEAGITCVIQPGGSMRDQEVIDAANEHGMAMIFTGMRHFRH

Sequences:

>Translated_530_residues
MNNARPIHRALLSVSDKTGIVEFAKALAERGVELLSTGGTARLLAEQGLTVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAVMNTHGIQPIDMVVVNLYPFAQTVANPNCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNAHDYDRVIREM
DANHNSLTLATRFDLAIAAFEHTAAYDGMIANYFGTLVPSYGDNKEGDEESKFPRTFNAQFIKKQDMRYGENSHQAAAFY
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGDDLLQAYNRAYQTDPTSAFGGI
IAFNRELDGETARAIIERQFVEVIIAPKVSQAAIDIVAAKQNVRLLECGEWQGQTTGFDLKRVNGGLLVQDRDQGMVAQD
DLQVVSTRQPSDAELKDALFCWKVAKYVKSNAIVYAKGDMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSVMASDAFF
PFRDGIDAAAEAGITCVIQPGGSMRDQEVIDAANEHGMAMIFTGMRHFRH
>Mature_530_residues
MNNARPIHRALLSVSDKTGIVEFAKALAERGVELLSTGGTARLLAEQGLTVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAVMNTHGIQPIDMVVVNLYPFAQTVANPNCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNAHDYDRVIREM
DANHNSLTLATRFDLAIAAFEHTAAYDGMIANYFGTLVPSYGDNKEGDEESKFPRTFNAQFIKKQDMRYGENSHQAAAFY
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGDDLLQAYNRAYQTDPTSAFGGI
IAFNRELDGETARAIIERQFVEVIIAPKVSQAAIDIVAAKQNVRLLECGEWQGQTTGFDLKRVNGGLLVQDRDQGMVAQD
DLQVVSTRQPSDAELKDALFCWKVAKYVKSNAIVYAKGDMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSVMASDAFF
PFRDGIDAAAEAGITCVIQPGGSMRDQEVIDAANEHGMAMIFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=488, Percent_Identity=37.2950819672131, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=79.84934086629, Blast_Score=876, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=527, Percent_Identity=33.0170777988615, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI71985574, Length=382, Percent_Identity=26.1780104712042, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=37.3913043478261, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323056, Length=500, Percent_Identity=34.2, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=34.4329896907216, Blast_Score=243, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24649832, Length=510, Percent_Identity=38.2352941176471, Blast_Score=275, Evalue=6e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57327; Mature: 57327

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNARPIHRALLSVSDKTGIVEFAKALAERGVELLSTGGTARLLAEQGLTVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHEECCCCCEEEHHHCCCEEEEECCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAVMNTHGIQPIDMVVVNLYPFAQTVANPNCTLA
HHHHCCCEEEECCHHHCCCCCCCCCCCCEECCCCCCHHHEEEEEHHHHHHHHCCCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVNAHDYDRVIREMDANHNSLTLATRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEEECCEEEEHH
EHTAAYDGMIANYFGTLVPSYGDNKEGDEESKFPRTFNAQFIKKQDMRYGENSHQAAAFY
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGDD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
LLQAYNRAYQTDPTSAFGGIIAFNRELDGETARAIIERQFVEVIIAPKVSQAAIDIVAAK
HHHHHHHHHCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHEEEEEC
QNVRLLECGEWQGQTTGFDLKRVNGGLLVQDRDQGMVAQDDLQVVSTRQPSDAELKDALF
CCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCEECCCHHHEECCCCCCCHHHHHHH
CWKVAKYVKSNAIVYAKGDMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSVMASDAFF
HHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
PFRDGIDAAAEAGITCVIQPGGSMRDQEVIDAANEHGMAMIFTGMRHFRH
CCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MNNARPIHRALLSVSDKTGIVEFAKALAERGVELLSTGGTARLLAEQGLTVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHEECCCCCEEEHHHCCCEEEEECCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAVMNTHGIQPIDMVVVNLYPFAQTVANPNCTLA
HHHHCCCEEEECCHHHCCCCCCCCCCCCEECCCCCCHHHEEEEEHHHHHHHHCCCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVNAHDYDRVIREMDANHNSLTLATRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEEEECCEEEEHH
EHTAAYDGMIANYFGTLVPSYGDNKEGDEESKFPRTFNAQFIKKQDMRYGENSHQAAAFY
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
VEANPQEASVATARQIQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVALGDD
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
LLQAYNRAYQTDPTSAFGGIIAFNRELDGETARAIIERQFVEVIIAPKVSQAAIDIVAAK
HHHHHHHHHCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHEEEEEC
QNVRLLECGEWQGQTTGFDLKRVNGGLLVQDRDQGMVAQDDLQVVSTRQPSDAELKDALF
CCCEEEECCCCCCCCCCCEEEECCCCEEEEECCCCCEECCCHHHEECCCCCCCHHHHHHH
CWKVAKYVKSNAIVYAKGDMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSVMASDAFF
HHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
PFRDGIDAAAEAGITCVIQPGGSMRDQEVIDAANEHGMAMIFTGMRHFRH
CCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA