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The map label for this gene is purD

Identifier: 227080489

GI number: 227080489

Start: 264266

End: 265555

Strand: Reverse

Name: purD

Synonym: VCM66_0260

Alternate gene names: 227080489

Gene position: 265555-264266 (Counterclockwise)

Preceding gene: 227080490

Following gene: 227080486

Centisome position: 9.18

GC content: 55.19

Gene sequence:

>1290_bases
ATGCAAGTTCTCATCATCGGCTCTGGCGGTCGCGAACACGCACTGGCGTGGAAAGTGGCACAAAACCCACAAGTTGACAC
CATCTACGTCGCGCCCGGCAATGCGGGTACTGCCCTTGAGCACAAGGTGCAAAACGTCAACATTGGCATCACCGACATTC
CAGCACTGGTTGCCTTTGCTCAAGATAAAGCGATTGAGCTGACCATCGTTGGCCCAGAAGCACCACTGGTGATTGGTGTG
GTGGATGCGTTTCGCGCTGCAGGTTTACCGATTTTTGGCCCAACCCAAGGCGCAGCGCAACTGGAAGGCTCCAAAGCCTT
CACTAAAGATTTCCTCGCTCGCCATAACATTCCAACCGCGGCTTACGCCAACTTCACTGAAATTGAGCCAGCTCTGGCTT
ATGTGCGTGAAAAAGGTGCGCCGATTGTAGTGAAAGCGGACGGTTTAGCGGCGGGCAAAGGCGTAATTGTGGCCATGACT
CTGCAAGAGGCCGAAGACGCAATTCAAGACATGTTGGCGGGTAATGCGTTTGGTTCAGCAGGCAGCCGTGTGGTGGTTGA
AGAATTCCTTGATGGAGAGGAAGCGAGCTTTATCGTGATGGTTGATGGTGAAAACGTCCTACCTATGGCCACCAGCCAAG
ATCACAAACGCGTGGGCGATGCCGATACTGGCCCGAACACCGGCGGTATGGGTGCTTACTCACCAGCGCCAGTGGTGACA
CAGGACGTCCATGATCGCGTAATGCGTGAAGTGATTGACCCAACCGTGCGCGGCATGGCAGCCGAAGGCAACACCTACAC
AGGCTTCCTCTACGCGGGTTTGATGATTGATAGCACTGGTGCACCGAAAGTGATTGAGTACAACTGCCGCTTTGGTGACC
CAGAAACCCAACCGATCATGATGCGTCTGCAATCGGATCTGGTTGAGCTGTGCCAAGCAGCGATTGCGGGCAAGCTGGAT
CAGGTGGAATCGAAGTGGGATCCACGCGCCTCGATCGGTGTGGTTCTCGCCGCTGGCGGCTACCCAGGCGACTACGCCAA
AGGTGAGGTGATTTCAGGTCTACCGACTCAAGAAAGCGCAGGACAAAAAGTGTTCCACGCAGGCACCGAAACCCAAGGCG
ACCAAGTGGTGACGAATGGCGGTCGCGTATTGTGTGCCACTGCACTCGGCAATACCGTATTAGAAGCGCAGCAACGCGCT
TACCAACTGGCGGATCAAATCCACTGGAATGGTATGTTCTGCCGCCGCGATATCGGTTATCGCGCGATTGCGCGTGAGCA
AGCCAAGTAA

Upstream 100 bases:

>100_bases
ATGGCGATGATCTTCACCGGTATGCGTCACTTCCGTCATTAATTGGCATGATTGGTATGGGGTGCAACGCGCCCCATCTT
GTTTAAGTAAGGATTTCACA

Downstream 100 bases:

>100_bases
GCGGGCATCCATCTTCTTATCAATAGAAAAAAGCGCGAACTTCGCGCTTTTTTATTCGATAGGTTTTTTATTCGATTAGA
TTGGCAAACATGGGCCGATT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFAQDKAIELTIVGPEAPLVIGV
VDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHNIPTAAYANFTEIEPALAYVREKGAPIVVKADGLAAGKGVIVAMT
LQEAEDAIQDMLAGNAFGSAGSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
QDVHDRVMREVIDPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIMMRLQSDLVELCQAAIAGKLD
QVESKWDPRASIGVVLAAGGYPGDYAKGEVISGLPTQESAGQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRA
YQLADQIHWNGMFCRRDIGYRAIAREQAK

Sequences:

>Translated_429_residues
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFAQDKAIELTIVGPEAPLVIGV
VDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHNIPTAAYANFTEIEPALAYVREKGAPIVVKADGLAAGKGVIVAMT
LQEAEDAIQDMLAGNAFGSAGSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
QDVHDRVMREVIDPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIMMRLQSDLVELCQAAIAGKLD
QVESKWDPRASIGVVLAAGGYPGDYAKGEVISGLPTQESAGQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRA
YQLADQIHWNGMFCRRDIGYRAIAREQAK
>Mature_429_residues
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFAQDKAIELTIVGPEAPLVIGV
VDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHNIPTAAYANFTEIEPALAYVREKGAPIVVKADGLAAGKGVIVAMT
LQEAEDAIQDMLAGNAFGSAGSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
QDVHDRVMREVIDPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIMMRLQSDLVELCQAAIAGKLD
QVESKWDPRASIGVVLAAGGYPGDYAKGEVISGLPTQESAGQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRA
YQLADQIHWNGMFCRRDIGYRAIAREQAK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=50.4672897196262, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=50.4672897196262, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=50.4672897196262, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=50.4672897196262, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=78.2710280373832, Blast_Score=694, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=41.9811320754717, Blast_Score=318, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=41.6666666666667, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.5813953488372, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.5813953488372, Blast_Score=358, Evalue=4e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_VIBCH (Q9KV81)

Other databases:

- EMBL:   AE003852
- PIR:   B82344
- RefSeq:   NP_229931.1
- ProteinModelPortal:   Q9KV81
- SMR:   Q9KV81
- GeneID:   2614998
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0275
- TIGR:   VC_0275
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 45309; Mature: 45309

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHEEEECCCHHHHHHHHHH
QDKAIELTIVGPEAPLVIGVVDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
AYANFTEIEPALAYVREKGAPIVVKADGLAAGKGVIVAMTLQEAEDAIQDMLAGNAFGSA
HCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
CCEEHHHHHCCCCCCEEEEEECCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCH
QDVHDRVMREVIDPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHCHHHHCEECCCCEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHH
MRLQSDLVELCQAAIAGKLDQVESKWDPRASIGVVLAAGGYPGDYAKGEVISGLPTQESA
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCC
GQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRAYQLADQIHWNGMFCRRDIGY
CCEEEECCCCCCCCEEEECCCEEEEEEHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
RAIAREQAK
HHHHHHCCC
>Mature Secondary Structure
MQVLIIGSGGREHALAWKVAQNPQVDTIYVAPGNAGTALEHKVQNVNIGITDIPALVAFA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHEEEECCCHHHHHHHHHH
QDKAIELTIVGPEAPLVIGVVDAFRAAGLPIFGPTQGAAQLEGSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
AYANFTEIEPALAYVREKGAPIVVKADGLAAGKGVIVAMTLQEAEDAIQDMLAGNAFGSA
HCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDADTGPNTGGMGAYSPAPVVT
CCEEHHHHHCCCCCCEEEEEECCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCH
QDVHDRVMREVIDPTVRGMAAEGNTYTGFLYAGLMIDSTGAPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHCHHHHCEECCCCEEEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHH
MRLQSDLVELCQAAIAGKLDQVESKWDPRASIGVVLAAGGYPGDYAKGEVISGLPTQESA
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCC
GQKVFHAGTETQGDQVVTNGGRVLCATALGNTVLEAQQRAYQLADQIHWNGMFCRRDIGY
CCEEEECCCCCCCCEEEECCCEEEEEEHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
RAIAREQAK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301