Click here to switch to the map view.

The map label for this gene is pyrE [H]

Identifier: 227080432

GI number: 227080432

Start: 204652

End: 205296

Strand: Direct

Name: pyrE [H]

Synonym: VCM66_0199

Alternate gene names: 227080432

Gene position: 204652-205296 (Clockwise)

Preceding gene: 227080431

Following gene: 227080436

Centisome position: 7.08

GC content: 48.06

Gene sequence:

>645_bases
ATGATGAAAGCTTACCAGCGTGAATTTATTGAGTTTGCCCTAGAAAAACAGGTGCTGAAGTTTGGCGAATTTACTTTGAA
ATCAGGCCGTAAAAGTCCTTATTTCTTTAACGCTGGTCTGTTTAATACTGGCCGTGATTTAGCGCGCTTGGGCCGTTTTT
ACGCTGCAGCGTTAGTTGATTCAGGTATTGAATTTGATGTGCTGTTTGGCCCTGCTTACAAAGGCATTCCGATTGCTACC
ACCACAGCAGTCGCATTGGCGGATCACCACGATGTGGATACGCCTTACTGCTTTAACCGCAAAGAGGCCAAAAATCACGG
CGAAGGCGGTAATCTAGTGGGCAGCAAGTTAGAAGGCCGCGTGATGTTGGTGGATGATGTGATCACCGCTGGTACGGCAA
TTCGTGAATCGATGGAGCTTATCCAAGCCAACAAGGCTGATTTAGCCGGCGTGCTAGTGGCGATCGATCGCCAAGAGAAA
GGCAAGGGCGAGCTCTCAGCGATTCAAGAAGTGGAACGTGATTTTGGTTGTGCGGTGATTTCGATTGTGAGCCTGACCGA
TCTCATCACCTATCTTGAACAGCAAGGTAACAACACTGAGCATCTAGAGGCGGTGAAAGCGTATCGTGCACAGTACGGAA
TTTAA

Upstream 100 bases:

>100_bases
GCACAGAACATCCCCCCTCTTTTAAAAAGAGACGGAACAATGTTTGAATTTCTCCCTGTGGGGAGGAAGTAAGTCCAAGT
GTATGAGTTAAGAGAGAGAA

Downstream 100 bases:

>100_bases
GCGTATTCATCCAACTAAGCCTAAAACGAAAAAACAGCCTCAAAATGAGGCTGTTTTTCTAGCATTAAGCTCAAGAACAA
AACGCTTAGCTTTGATCGCC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MMKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDSGIEFDVLFGPAYKGIPIAT
TTAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSKLEGRVMLVDDVITAGTAIRESMELIQANKADLAGVLVAIDRQEK
GKGELSAIQEVERDFGCAVISIVSLTDLITYLEQQGNNTEHLEAVKAYRAQYGI

Sequences:

>Translated_214_residues
MMKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDSGIEFDVLFGPAYKGIPIAT
TTAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSKLEGRVMLVDDVITAGTAIRESMELIQANKADLAGVLVAIDRQEK
GKGELSAIQEVERDFGCAVISIVSLTDLITYLEQQGNNTEHLEAVKAYRAQYGI
>Mature_214_residues
MMKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDSGIEFDVLFGPAYKGIPIAT
TTAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSKLEGRVMLVDDVITAGTAIRESMELIQANKADLAGVLVAIDRQEK
GKGELSAIQEVERDFGCAVISIVSLTDLITYLEQQGNNTEHLEAVKAYRAQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=181, Percent_Identity=28.7292817679558, Blast_Score=85, Evalue=5e-17,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=81.6901408450704, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17508631, Length=163, Percent_Identity=28.8343558282209, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=195, Percent_Identity=25.1282051282051, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=221, Percent_Identity=50.2262443438914, Blast_Score=211, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6323927, Length=223, Percent_Identity=46.1883408071749, Blast_Score=194, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23533; Mature: 23533

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
SGIEFDVLFGPAYKGIPIATTTAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSKLEGR
CCCEEEEEECCCCCCCCCHHHHHHHECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
VMLVDDVITAGTAIRESMELIQANKADLAGVLVAIDRQEKGKGELSAIQEVERDFGCAVI
EEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCHHHH
SIVSLTDLITYLEQQGNNTEHLEAVKAYRAQYGI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKAYQREFIEFALEKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
SGIEFDVLFGPAYKGIPIATTTAVALADHHDVDTPYCFNRKEAKNHGEGGNLVGSKLEGR
CCCEEEEEECCCCCCCCCHHHHHHHECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
VMLVDDVITAGTAIRESMELIQANKADLAGVLVAIDRQEKGKGELSAIQEVERDFGCAVI
EEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCHHHH
SIVSLTDLITYLEQQGNNTEHLEAVKAYRAQYGI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]