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The map label for this gene is rph [H]

Identifier: 227080431

GI number: 227080431

Start: 203516

End: 204289

Strand: Direct

Name: rph [H]

Synonym: VCM66_0198

Alternate gene names: 227080431

Gene position: 203516-204289 (Clockwise)

Preceding gene: 227080428

Following gene: 227080432

Centisome position: 7.04

GC content: 52.71

Gene sequence:

>774_bases
TTGGGCTATAATCCTGCCCCAACCGAATTGATACAGCCACAAGAAGGTCGAGACCCTATGCGTCCAGATAACCGCGCTGC
GGACCAAGTTCGCCCCATCAAAATCACCCGTCACTACACTGCGTACGCAGAAGGCTCGGTGTTGGTGGAATTTGGCAACA
CCAAAGTGTTGTGTAATGCCAGCATTGAAGAAGGTGTGCCACGTTGGTTAAAAGGCCAAGGTAAAGGCTGGGTGACGGCG
GAATACGGCATGTTGCCGCGTGCAACCCATTCACGCACGCGCCGTGAAGCGACCAATGGTAAGCAAGGCGGTCGTACCAT
GGAAATCCAGCGTCTGATCGCGCGCAGCCTACGTGCTGTGGTGGATCTGGAAGCGATGGGCGAAATCATGATCACCGTTG
ATTGTGATGTGATTCAAGCCGATGGCGGTACGCGTACCGCTTCGATTACTGGTGCAAGCGTGGCACTGGCCGATGCTTTT
GCCCATCTGATCGCCAAAGGCCAACTGAAGAAGAACCCGATGAAAGGCCATGTGGCTGCGGTTTCGGTTGGTATCCTAGG
TGAAGATGTGCTGTGCGATCTGGAATATGTCGAAGATTCTGCCGCTGATACGGATATGAACGTGGTCATGACCGAAGAAG
GCAAAATGATTGAAATTCAAGGCACTGCTGAAGGTGAGCCGTTCAGTCATGAGCAGTTGCTGGAATTGCTGGCTGTCGCG
AAAAAAGGCATTGCCGATATTGTCGCAGTGCAGAAGGCATCGTTGTTAGATTGA

Upstream 100 bases:

>100_bases
GTCCTTTGCTTTCATCTCTTTGTGACTGGCGCTAATAGTAGCAAAGCAGGCAAAACGGCGCTACCGTGCTGAATTCGTTA
ACCTAGCGCCTGTAGGGGCT

Downstream 100 bases:

>100_bases
TTAAGTAGCTCCCAATTGGGGGCTATTTTTTTGCCTAGGAAATCCCTTTTTGGACGATGCCTGATCCTCATCGCCTTCTA
GGGGGAATTAGCCTTAAGTG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MGYNPAPTELIQPQEGRDPMRPDNRAADQVRPIKITRHYTAYAEGSVLVEFGNTKVLCNASIEEGVPRWLKGQGKGWVTA
EYGMLPRATHSRTRREATNGKQGGRTMEIQRLIARSLRAVVDLEAMGEIMITVDCDVIQADGGTRTASITGASVALADAF
AHLIAKGQLKKNPMKGHVAAVSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLELLAVA
KKGIADIVAVQKASLLD

Sequences:

>Translated_257_residues
MGYNPAPTELIQPQEGRDPMRPDNRAADQVRPIKITRHYTAYAEGSVLVEFGNTKVLCNASIEEGVPRWLKGQGKGWVTA
EYGMLPRATHSRTRREATNGKQGGRTMEIQRLIARSLRAVVDLEAMGEIMITVDCDVIQADGGTRTASITGASVALADAF
AHLIAKGQLKKNPMKGHVAAVSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLELLAVA
KKGIADIVAVQKASLLD
>Mature_256_residues
GYNPAPTELIQPQEGRDPMRPDNRAADQVRPIKITRHYTAYAEGSVLVEFGNTKVLCNASIEEGVPRWLKGQGKGWVTAE
YGMLPRATHSRTRREATNGKQGGRTMEIQRLIARSLRAVVDLEAMGEIMITVDCDVIQADGGTRTASITGASVALADAFA
HLIAKGQLKKNPMKGHVAAVSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLELLAVAK
KGIADIVAVQKASLLD

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=74.8815165876777, Blast_Score=340, Evalue=7e-95,
Organism=Caenorhabditis elegans, GI71981632, Length=181, Percent_Identity=29.2817679558011, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27727; Mature: 27596

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYNPAPTELIQPQEGRDPMRPDNRAADQVRPIKITRHYTAYAEGSVLVEFGNTKVLCNA
CCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCEEEEEEEEEEEECCEEEEEECCEEEEEEC
SIEEGVPRWLKGQGKGWVTAEYGMLPRATHSRTRREATNGKQGGRTMEIQRLIARSLRAV
CHHHCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VDLEAMGEIMITVDCDVIQADGGTRTASITGASVALADAFAHLIAKGQLKKNPMKGHVAA
HHHHHCCCEEEEEECEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLELLAVA
EEEEECCCCCEEEHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHH
KKGIADIVAVQKASLLD
HHCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GYNPAPTELIQPQEGRDPMRPDNRAADQVRPIKITRHYTAYAEGSVLVEFGNTKVLCNA
CCCCCCHHHCCCCCCCCCCCCCCCCHHCCCEEEEEEEEEEEECCEEEEEECCEEEEEEC
SIEEGVPRWLKGQGKGWVTAEYGMLPRATHSRTRREATNGKQGGRTMEIQRLIARSLRAV
CHHHCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VDLEAMGEIMITVDCDVIQADGGTRTASITGASVALADAFAHLIAKGQLKKNPMKGHVAA
HHHHHCCCEEEEEECEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VSVGILGEDVLCDLEYVEDSAADTDMNVVMTEEGKMIEIQGTAEGEPFSHEQLLELLAVA
EEEEECCCCCEEEHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHH
KKGIADIVAVQKASLLD
HHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]