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The map label for this gene is murI

Identifier: 227080392

GI number: 227080392

Start: 149249

End: 150046

Strand: Direct

Name: murI

Synonym: VCM66_0157

Alternate gene names: 227080392

Gene position: 149249-150046 (Clockwise)

Preceding gene: 227080390

Following gene: 227080395

Centisome position: 5.16

GC content: 47.62

Gene sequence:

>798_bases
GTGTCATCTCCATCTTTTCCTCGTGTTCTGATTTTCGATTCTGGTGTGGGTGGTCTTTCCGTATATCGAGAAATTGAAGC
CCGCTTGCCGCAATTAAACTATATCTATCTGTTTGATAACGCGGCATATCCTTATGGTGAGCTGACCCAGGAAACACTGA
TCGCACGAGTTGATACGCTGGTTACTCGAATGGTTGAACAAGAGCGAATCGATCTTGTGGTCATCGCTTGTAATACCGCG
AGCACCATAGTGTTACCCGTATTGCGCGCCAAACTCACGATACCTGTGGTCGGGGTTGTGCCTGCAATTAAACCCGCCTC
CCTGATTGCTAGCAAGGCGATTGGGCTTATCGCGACGCCAGCCACAGTGAAACGGCAATACACTCAAGAACTGATCCGTG
ACTTTTCCGCCAACAAAAACGTTGAGCTATTGGGGTCTACACGCTTGGTTAATATGGCTGAAGAGAAATTGCGTGGTAAG
CCTCTCGATCTCGAAGAATTAGCCAGCATATTGCAACCCCTGAAGAACACGATTGATGTCGCCGTTTTAGGCTGTACTCA
CTTTCCCCTGATTAAAGAAGAGATCCAGCAAGTACTTGGAGAGCAAGTGCAACTGATTGATTCTGGTTTGGCCATTGCGC
GCAGAGTGCAGGAGTTGCTGGGAATAGAGCAAGCGGTTGGTGCAAAGCAAAAGCATCGAATTTATGCAAGCGCACCACCA
TGGGAGGAAAGTGCGCTGAACATAAAGCTTGAACAGTTAGGTTTTAATCCTGTTCAGCCATTTCTTCATCCGATTTAG

Upstream 100 bases:

>100_bases
TTTGAATTAGATTTTTTAATGCTATATATCTGGGGTGGATTGCCTAATGTAGCAGCGAGAGTATGATTTCTCCGTTTTTT
CTAGTTAGGTTGTCGCACTC

Downstream 100 bases:

>100_bases
GATCATTCGCAATGCGAACTTTTAACGTTCGCTGCATATATTCCTTTTCGTTGAGTGCATCAATGGCTGTTTGAGCTTGG
CTTGCGGCTATCACCACAAA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSSPSFPRVLIFDSGVGGLSVYREIEARLPQLNYIYLFDNAAYPYGELTQETLIARVDTLVTRMVEQERIDLVVIACNTA
STIVLPVLRAKLTIPVVGVVPAIKPASLIASKAIGLIATPATVKRQYTQELIRDFSANKNVELLGSTRLVNMAEEKLRGK
PLDLEELASILQPLKNTIDVAVLGCTHFPLIKEEIQQVLGEQVQLIDSGLAIARRVQELLGIEQAVGAKQKHRIYASAPP
WEESALNIKLEQLGFNPVQPFLHPI

Sequences:

>Translated_265_residues
MSSPSFPRVLIFDSGVGGLSVYREIEARLPQLNYIYLFDNAAYPYGELTQETLIARVDTLVTRMVEQERIDLVVIACNTA
STIVLPVLRAKLTIPVVGVVPAIKPASLIASKAIGLIATPATVKRQYTQELIRDFSANKNVELLGSTRLVNMAEEKLRGK
PLDLEELASILQPLKNTIDVAVLGCTHFPLIKEEIQQVLGEQVQLIDSGLAIARRVQELLGIEQAVGAKQKHRIYASAPP
WEESALNIKLEQLGFNPVQPFLHPI
>Mature_264_residues
SSPSFPRVLIFDSGVGGLSVYREIEARLPQLNYIYLFDNAAYPYGELTQETLIARVDTLVTRMVEQERIDLVVIACNTAS
TIVLPVLRAKLTIPVVGVVPAIKPASLIASKAIGLIATPATVKRQYTQELIRDFSANKNVELLGSTRLVNMAEEKLRGKP
LDLEELASILQPLKNTIDVAVLGCTHFPLIKEEIQQVLGEQVQLIDSGLAIARRVQELLGIEQAVGAKQKHRIYASAPPW
EESALNIKLEQLGFNPVQPFLHPI

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=266, Percent_Identity=49.6240601503759, Blast_Score=217, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_VIBC3 (A5F4P4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4P4
- SMR:   A5F4P4
- STRING:   A5F4P4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2339
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- BioCyc:   VCHO345073:VC0395_A2339-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29197; Mature: 29065

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSFPRVLIFDSGVGGLSVYREIEARLPQLNYIYLFDNAAYPYGELTQETLIARVDTL
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
VTRMVEQERIDLVVIACNTASTIVLPVLRAKLTIPVVGVVPAIKPASLIASKAIGLIATP
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHEECC
ATVKRQYTQELIRDFSANKNVELLGSTRLVNMAEEKLRGKPLDLEELASILQPLKNTIDV
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHE
AVLGCTHFPLIKEEIQQVLGEQVQLIDSGLAIARRVQELLGIEQAVGAKQKHRIYASAPP
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHCEEEECCCC
WEESALNIKLEQLGFNPVQPFLHPI
CCCCCEEEEHHHCCCCCCCHHHCCC
>Mature Secondary Structure 
SSPSFPRVLIFDSGVGGLSVYREIEARLPQLNYIYLFDNAAYPYGELTQETLIARVDTL
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
VTRMVEQERIDLVVIACNTASTIVLPVLRAKLTIPVVGVVPAIKPASLIASKAIGLIATP
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHEECC
ATVKRQYTQELIRDFSANKNVELLGSTRLVNMAEEKLRGKPLDLEELASILQPLKNTIDV
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHE
AVLGCTHFPLIKEEIQQVLGEQVQLIDSGLAIARRVQELLGIEQAVGAKQKHRIYASAPP
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHCEEEECCCC
WEESALNIKLEQLGFNPVQPFLHPI
CCCCCEEEEHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA