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The map label for this gene is btuB

Identifier: 227080390

GI number: 227080390

Start: 145341

End: 147176

Strand: Direct

Name: btuB

Synonym: VCM66_0155

Alternate gene names: 227080390

Gene position: 145341-147176 (Clockwise)

Preceding gene: 227080388

Following gene: 227080392

Centisome position: 5.02

GC content: 43.25

Gene sequence:

>1836_bases
ATGCAAAAATCAGCACTGGCGATCGCTCTAGCATCGCTGCTTACCCCTATCTCTTATTTACACGCCAACGAAGCACAACC
TCAAGAAACGGTTGTTGTGACGGCAAATCGTTTTGAGCAAAAGGCCAGTTCAACTTTGGCGGATGTGGAAATCATTACCC
GCCAAGATATCGAACAAACTCAAGCTAAGACATTGCCTGAGTTGTTACGTAGATTAACGGGTGTTCAGATCACGCAAAAT
GGTGGACGTGGTCAGTTAGCCTCTTTATTCGTACGTGGAACAAGTTCAGATCAAGTATTGGTATTAGTGGATGGAATTCG
TTTTGCTCGTGCTGCAAAAGGAGCGGTCGATTTCAACCAAATTCCACTAACTTATGTTGACCGTATTGAGTATGTGCGTG
GCGCTCGAGCTTCGTTATATGGATCTGAAGCGATTGGTGGTGTCATTAACATCATTACTAAAGCTCGTTCACAACAACAA
GGCACAACCGTAAGTGCTGGACTGGGTTCATTGGATTACCAAGAACTCAGTATTGCTTCTGGGGTTGCGATTGGTGAAAA
AGGCCAGATGAATGTGGCTTTGGGCACAGAGAGTGATAAAGGGTACAACGTTCGTCCAGTTCCTGGAGTGAATGATGGTG
ATCGTCACGGTTTCAGGTCAGATAATGCGTTGTTGGGTTACGTGCACCAATTTGATGAGTCTTGGTCTTTGTTTGCCAAT
GCCCGCGCTTATGAAAATATTTATCAGTACGACAACAGCTATGGTACTCGTGACTATAAAGAGGCAGAAAAGGATGACCT
TTCTTTTACAATTGGTACTCAGTATCAAAGTGAACGTTGGGTATCTGAATTGCAGCTAACTACCCAGAAGCAAAAATCAT
GGGATTACACCCAGTCTAAGGGAAAATACTCGGACACATCGGATAATCTTGAACAACGAAATATCCAGTGGATTAATCGC
TATTTGGTCAATGATGTGTGGACGTTCGCTGGTGGTGTGGACTGGCGAGACGAGTCGTATATTGATAAAACAGCGGATAA
AGAGTTTGACCGCAGCAACACTGCTGCATTTGCTGTGGTTGCTGCTGAGTGGCAACAATGGCTGTTGGAGGCAAGTCTAC
GCTTTGATGATAACCAAGAGTATGGTTCGCAAACTACCCACAACATTGCTCTAGGGTATCAGTTTATCCCTGAGTTCGGG
GTTAAAGCATCTTATGGCTCAGCATTTAAGGCTCCCAATTTATATCAACAGTACGATCCGTCTTATGGCAATGTTAATTT
ACAACCGGAAGACGCAGATTCAGCAGAGTTGAGCTTCTACGGCTTATTTTCAGGTATTAAATGGTCTATCACTGGCTATG
ATTACAAAATCAATAACCTGATCGATTACAACTCAACAACGAAGAATTACCAAAACGTAATAGGTGAGAGCAATATAAAA
GGAGTTGAGTTTACAGCCGAGTTTGCCACTGGAATTGTGCAGCACCAGTTAAGTGTTGACCTCAAAGATGCCGATGATAG
TAAAGGGAAAACATTACAGCGTCGTGCTGAGCATATGTATAAGTGGAATGCGCTAGTGGCTTTCGAACAAGTGGATTGGT
CAATTGGTTACCAATATGTAGGTAAACGCCCAGATTTGGACTACAACACTTACCCCACTCAAAACATAACACTTGATGCG
TATTCTCTCGTTGATACCTCAGTTAGCTACTATGTCACAGACTCGACGACTATTAGCGCTCGAATTGATAACTTATTAGA
TAAAGAGTACGAAACCGCCAATGGCTACCCTGCTGCTGAGCGCGCGTATTATCTTAATATTGGTTATCAGTTTTAA

Upstream 100 bases:

>100_bases
CCAGTAGGCCAACAGTGCTCTCAAGTCCGAAGACCTGCCAGCAACTGAGTTATGCAGTGGTATTGCACTCGGTAGGATTC
ACGCGATTTAGGACAACATA

Downstream 100 bases:

>100_bases
ACTTAGACACTGTCTTTAATCACAAAAGCCAGTCAGAACATCTGACTGGCTTTTATTGTTTAATGCGTCATGTTAGTAGC
GAGCCACCAAACTGACATTG

Product: vitamin B12 receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MQKSALAIALASLLTPISYLHANEAQPQETVVVTANRFEQKASSTLADVEIITRQDIEQTQAKTLPELLRRLTGVQITQN
GGRGQLASLFVRGTSSDQVLVLVDGIRFARAAKGAVDFNQIPLTYVDRIEYVRGARASLYGSEAIGGVINIITKARSQQQ
GTTVSAGLGSLDYQELSIASGVAIGEKGQMNVALGTESDKGYNVRPVPGVNDGDRHGFRSDNALLGYVHQFDESWSLFAN
ARAYENIYQYDNSYGTRDYKEAEKDDLSFTIGTQYQSERWVSELQLTTQKQKSWDYTQSKGKYSDTSDNLEQRNIQWINR
YLVNDVWTFAGGVDWRDESYIDKTADKEFDRSNTAAFAVVAAEWQQWLLEASLRFDDNQEYGSQTTHNIALGYQFIPEFG
VKASYGSAFKAPNLYQQYDPSYGNVNLQPEDADSAELSFYGLFSGIKWSITGYDYKINNLIDYNSTTKNYQNVIGESNIK
GVEFTAEFATGIVQHQLSVDLKDADDSKGKTLQRRAEHMYKWNALVAFEQVDWSIGYQYVGKRPDLDYNTYPTQNITLDA
YSLVDTSVSYYVTDSTTISARIDNLLDKEYETANGYPAAERAYYLNIGYQF

Sequences:

>Translated_611_residues
MQKSALAIALASLLTPISYLHANEAQPQETVVVTANRFEQKASSTLADVEIITRQDIEQTQAKTLPELLRRLTGVQITQN
GGRGQLASLFVRGTSSDQVLVLVDGIRFARAAKGAVDFNQIPLTYVDRIEYVRGARASLYGSEAIGGVINIITKARSQQQ
GTTVSAGLGSLDYQELSIASGVAIGEKGQMNVALGTESDKGYNVRPVPGVNDGDRHGFRSDNALLGYVHQFDESWSLFAN
ARAYENIYQYDNSYGTRDYKEAEKDDLSFTIGTQYQSERWVSELQLTTQKQKSWDYTQSKGKYSDTSDNLEQRNIQWINR
YLVNDVWTFAGGVDWRDESYIDKTADKEFDRSNTAAFAVVAAEWQQWLLEASLRFDDNQEYGSQTTHNIALGYQFIPEFG
VKASYGSAFKAPNLYQQYDPSYGNVNLQPEDADSAELSFYGLFSGIKWSITGYDYKINNLIDYNSTTKNYQNVIGESNIK
GVEFTAEFATGIVQHQLSVDLKDADDSKGKTLQRRAEHMYKWNALVAFEQVDWSIGYQYVGKRPDLDYNTYPTQNITLDA
YSLVDTSVSYYVTDSTTISARIDNLLDKEYETANGYPAAERAYYLNIGYQF
>Mature_611_residues
MQKSALAIALASLLTPISYLHANEAQPQETVVVTANRFEQKASSTLADVEIITRQDIEQTQAKTLPELLRRLTGVQITQN
GGRGQLASLFVRGTSSDQVLVLVDGIRFARAAKGAVDFNQIPLTYVDRIEYVRGARASLYGSEAIGGVINIITKARSQQQ
GTTVSAGLGSLDYQELSIASGVAIGEKGQMNVALGTESDKGYNVRPVPGVNDGDRHGFRSDNALLGYVHQFDESWSLFAN
ARAYENIYQYDNSYGTRDYKEAEKDDLSFTIGTQYQSERWVSELQLTTQKQKSWDYTQSKGKYSDTSDNLEQRNIQWINR
YLVNDVWTFAGGVDWRDESYIDKTADKEFDRSNTAAFAVVAAEWQQWLLEASLRFDDNQEYGSQTTHNIALGYQFIPEFG
VKASYGSAFKAPNLYQQYDPSYGNVNLQPEDADSAELSFYGLFSGIKWSITGYDYKINNLIDYNSTTKNYQNVIGESNIK
GVEFTAEFATGIVQHQLSVDLKDADDSKGKTLQRRAEHMYKWNALVAFEQVDWSIGYQYVGKRPDLDYNTYPTQNITLDA
YSLVDTSVSYYVTDSTTISARIDNLLDKEYETANGYPAAERAYYLNIGYQF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily

Homologues:

Organism=Escherichia coli, GI1790405, Length=621, Percent_Identity=38.9694041867955, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1788478, Length=663, Percent_Identity=26.6968325791855, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790743, Length=161, Percent_Identity=32.9192546583851, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786798, Length=161, Percent_Identity=31.6770186335404, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): BTUB_VIBC3 (A5F4P6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4P6
- SMR:   A5F4P6
- STRING:   A5F4P6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2342
- eggNOG:   COG4206
- HOGENOM:   HBG644936
- OMA:   HYETARW
- ProtClustDB:   CLSK873885
- BioCyc:   VCHO345073:VC0395_A2342-MONOMER
- HAMAP:   MF_01531
- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917
- Gene3D:   G3DSA:2.170.130.10
- Gene3D:   G3DSA:2.40.170.20
- TIGRFAMs:   TIGR01779

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec

EC number: NA

Molecular weight: Translated: 68610; Mature: 68610

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00430 TONB_DEPENDENT_REC_1; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSALAIALASLLTPISYLHANEAQPQETVVVTANRFEQKASSTLADVEIITRQDIEQT
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKTLPELLRRLTGVQITQNGGRGQLASLFVRGTSSDQVLVLVDGIRFARAAKGAVDFNQ
HHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHCCCCCCCC
IPLTYVDRIEYVRGARASLYGSEAIGGVINIITKARSQQQGTTVSAGLGSLDYQELSIAS
CCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHC
GVAIGEKGQMNVALGTESDKGYNVRPVPGVNDGDRHGFRSDNALLGYVHQFDESWSLFAN
CCEECCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCEEHHHHHHCCCHHHHHH
ARAYENIYQYDNSYGTRDYKEAEKDDLSFTIGTQYQSERWVSELQLTTQKQKSWDYTQSK
HHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GKYSDTSDNLEQRNIQWINRYLVNDVWTFAGGVDWRDESYIDKTADKEFDRSNTAAFAVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEE
AAEWQQWLLEASLRFDDNQEYGSQTTHNIALGYQFIPEFGVKASYGSAFKAPNLYQQYDP
HHHHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEEEHHCCCCCCCCCCCCCCCCCHHHCCC
SYGNVNLQPEDADSAELSFYGLFSGIKWSITGYDYKINNLIDYNSTTKNYQNVIGESNIK
CCCCEEECCCCCCCCCEEEEEECCCCEEEEECCEEEECCEEECCCCCHHHHHHCCCCCCC
GVEFTAEFATGIVQHQLSVDLKDADDSKGKTLQRRAEHMYKWNALVAFEQVDWSIGYQYV
CEEEEHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEEECCHHCCHHCC
GKRPDLDYNTYPTQNITLDAYSLVDTSVSYYVTDSTTISARIDNLLDKEYETANGYPAAE
CCCCCCCCCCCCCCCEEEEEHHHHCCCEEEEEECCCEEHHHHHHHHHHHHHCCCCCCCCC
RAYYLNIGYQF
CEEEEEEEECC
>Mature Secondary Structure
MQKSALAIALASLLTPISYLHANEAQPQETVVVTANRFEQKASSTLADVEIITRQDIEQT
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKTLPELLRRLTGVQITQNGGRGQLASLFVRGTSSDQVLVLVDGIRFARAAKGAVDFNQ
HHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHCCCCCCCC
IPLTYVDRIEYVRGARASLYGSEAIGGVINIITKARSQQQGTTVSAGLGSLDYQELSIAS
CCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHC
GVAIGEKGQMNVALGTESDKGYNVRPVPGVNDGDRHGFRSDNALLGYVHQFDESWSLFAN
CCEECCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCEEHHHHHHCCCHHHHHH
ARAYENIYQYDNSYGTRDYKEAEKDDLSFTIGTQYQSERWVSELQLTTQKQKSWDYTQSK
HHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GKYSDTSDNLEQRNIQWINRYLVNDVWTFAGGVDWRDESYIDKTADKEFDRSNTAAFAVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEE
AAEWQQWLLEASLRFDDNQEYGSQTTHNIALGYQFIPEFGVKASYGSAFKAPNLYQQYDP
HHHHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEEEHHCCCCCCCCCCCCCCCCCHHHCCC
SYGNVNLQPEDADSAELSFYGLFSGIKWSITGYDYKINNLIDYNSTTKNYQNVIGESNIK
CCCCEEECCCCCCCCCEEEEEECCCCEEEEECCEEEECCEEECCCCCHHHHHHCCCCCCC
GVEFTAEFATGIVQHQLSVDLKDADDSKGKTLQRRAEHMYKWNALVAFEQVDWSIGYQYV
CEEEEHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHEEEEEEECCHHCCHHCC
GKRPDLDYNTYPTQNITLDAYSLVDTSVSYYVTDSTTISARIDNLLDKEYETANGYPAAE
CCCCCCCCCCCCCCCEEEEEHHHHCCCEEEEEECCCEEHHHHHHHHHHHHHCCCCCCCCC
RAYYLNIGYQF
CEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA