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The map label for this gene is dapF [H]

Identifier: 227080362

GI number: 227080362

Start: 120417

End: 121289

Strand: Direct

Name: dapF [H]

Synonym: VCM66_0126

Alternate gene names: 227080362

Gene position: 120417-121289 (Clockwise)

Preceding gene: 227080361

Following gene: 227080363

Centisome position: 4.16

GC content: 51.78

Gene sequence:

>873_bases
GTGGGCGTTAGAATCGGTATTACCGGAGTAAAGTTATCTTCTATGCATTTCCATTTTTCTAAAATGCACGGTTTGGGCAA
CGACTTTATGGTCGTGGACTGTATTACCCAAAACGTCTTCTTCTCTCCGGAATTGATCCGCCGTCTGGCGGATCGCCATA
CGGGAGTCGGCTTTGATCAATTGTTGGTGGTTGAAGCGCCTTACGATCCAGAATCGGATTTCCATTACCGCATTTTCAAT
GCCGATGGCAGCGAAGTGGAGCAGTGTGGCAATGGCGCGCGCTGTTTTGCCCGTTTTGTACGCATGAAAGGGTTAACCAA
CAAGTACACCATCCATGTCAGTACCAAGAAAGGCAAAATGGTACTCAATGTGGAAGAGGAAGATCTGATCACCGTGAACA
TGGGTGTGCCGGAGTTTGAGCCGAACAAGATCCCATTTCGCGCCAAACAGAGCGAAAAAACCTACATTCTGCGTGTCGGT
GAACACACCTTGTTTTGCGGCGCGGTGAGTATGGGCAACCCGCATGTAGTGACTGTGGTGGACGATATCCGCACTGCTGC
GGTAGAAACCTTAGGGCCGCTTTTGGAATCGCACGAGCGTTTCCCTGAGCGCGTCAACGCCGGTTTTATGCAGGTCGTGA
GCCGTGATGAGATCAATTTGCGCGTGTATGAACGTGGTGCGGGCGAAACTCAAGCTTGTGGCAGTGGCGCGTGCGCCGCG
GTTGCGGTAGGCATTTTGCAAGGTTTACTGGATGAGCAAGTTCGTGTCCATCTTCCCGGTGGTGAGCTGGAAATCCACTG
GCAAGGCCCCGGCAAACCGCTGTATATGACAGGCCCGGCTACTCATATCTACGATGGCCAAATCTCTTGCTAA

Upstream 100 bases:

>100_bases
CCATGTCGTCGAATTACAATACCCGTCCGCGAGTGGCTGAAGTCATGGTTGATGGCAATAAAACCTATTTGGTGCGTCAG
CGTGAAGAGCTTAGCAGCTT

Downstream 100 bases:

>100_bases
TTGAGGTTCAATTTTGTCTCAGGTAACGGCGGATGCTCTGACTGCCCAAGTGGTGGCAGAGTATCTTTATGAACATCCCG
ATTTTTTTCAGCATCATCCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MGVRIGITGVKLSSMHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDFHYRIFN
ADGSEVEQCGNGARCFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVG
EHTLFCGAVSMGNPHVVTVVDDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAA
VAVGILQGLLDEQVRVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC

Sequences:

>Translated_290_residues
MGVRIGITGVKLSSMHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDFHYRIFN
ADGSEVEQCGNGARCFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVG
EHTLFCGAVSMGNPHVVTVVDDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAA
VAVGILQGLLDEQVRVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC
>Mature_289_residues
GVRIGITGVKLSSMHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDFHYRIFNA
DGSEVEQCGNGARCFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVGE
HTLFCGAVSMGNPHVVTVVDDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAV
AVGILQGLLDEQVRVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=272, Percent_Identity=77.9411764705882, Blast_Score=450, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32051; Mature: 31920

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVRIGITGVKLSSMHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQ
CCEEEEEEEEEEECEEEEHHHHCCCCCCEEEEEEECCCCEECHHHHHHHHHHCCCCCCCE
LLVVEAPYDPESDFHYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYTIHVSTKKGKM
EEEEECCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCEE
VLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVGEHTLFCGAVSMGNPHVVTVV
EEEECCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEE
DDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHH
VAVGILQGLLDEQVRVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC
HHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCEEEECCCCEEECCEECC
>Mature Secondary Structure 
GVRIGITGVKLSSMHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQ
CEEEEEEEEEEECEEEEHHHHCCCCCCEEEEEEECCCCEECHHHHHHHHHHCCCCCCCE
LLVVEAPYDPESDFHYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYTIHVSTKKGKM
EEEEECCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCEE
VLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVGEHTLFCGAVSMGNPHVVTVV
EEEECCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEE
DDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHH
VAVGILQGLLDEQVRVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC
HHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCEEEECCCCEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA