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The map label for this gene is recF

Identifier: 227080251

GI number: 227080251

Start: 9991

End: 11082

Strand: Direct

Name: recF

Synonym: VCM66_0014

Alternate gene names: 227080251

Gene position: 9991-11082 (Clockwise)

Preceding gene: 227080250

Following gene: 227080252

Centisome position: 0.35

GC content: 47.53

Gene sequence:

>1092_bases
ATGCCGCTTTCCCGGTTAATGATCCAACAGTTTCGTAATATTAAAGCCTGTGATATTCGCCTATCAGCAGGCTTTAACTT
TCTTATCGGGCCGAATGGCAGCGGTAAAACCAGCGTGCTTGAAGCGATTTATCTGCTCGGCCATGGCCGTTCATTTAAAA
GCTCGCTGACCGGACGGATCATTCAAAATGAGTGCTCAGAACTGTTTGTGCATGGCCGGATTTGTGAGCATTCTTTGAGC
TCTGATCAATTTGAGCTACCCGTTGGCATTAATAAGCAGCGTGACGGCTCAACTGAGGTTAAAATAGGCGGCCAAACAGG
GCAAAAATTGGCGCAATTAGCGCAGATTTTGCCACTGCAATTGATTCATCCGGAAGGCTTTGAACTGCTGACCGATGGAC
CGAAACAGCGTCGGGCTTTTATCGATTGGGGCGTGTTTCATACTGAGCCCGCTTTTTTCGATGCATGGGGGCGGTTTAAG
CGGCTCAGCAAGCAGCGTAATGCACTGCTAAAAAGCGCGCAAAGCTATCGTGAACTCAGTTATTGGGATCAAGAATTAGC
CCGTTTGGCTGAGCAGATTGACCAGTGGCGTGAAAGTTACGTCAATCAATTGAAAAATGTGGCAGAGCAGTTATGTCGCA
CATTTTTGCCAGAATTCGATATCGACTTAAAGTATTATCGAGGTTGGGAAAAAGATCAGCCTTATCAATCGATTCTGGAA
AAAAACTTCGAACGGGATCAGCAGTTGGGCTATACCTTTAGCGGGCCTAACAAAGCGGATTTGCGGATTAAAGTGAACGC
AACCCCGGTGGAAGATGTGTTGTCGCGGGGACAACTGAAGTTGATGGTGTGTGCGTTGCGCGTGGCGCAAGGGCAGCACC
TGACCGAGTTGACGGGAAAGCAATGCATTTATCTTATCGATGATTTTGCTTCCGAATTGGATAGCCTACGTCGCCAACGT
TTGGCAGATAGCCTAAAAGGGACGGGGGCGCAGGTTTTTGTAAGTTCTATTACCGAAAGCCAAGTGGCGGACATGTTAGA
TGAGTCCAGTAAAACATTTCACGTTGCCCACGGTGTAATAGAGCAAGGATAA

Upstream 100 bases:

>100_bases
TGTCGGATGCCAACGCCAGTGCCTTGGTGGAGAATGTCGATGATGACAGTGCCATGTACGTGGTCATGCCAATTCGTCTG
TAAGGTATAACCGAGTTACC

Downstream 100 bases:

>100_bases
ACAGAGTGAGAGAGTAACTCATGTCGAACAATTACGATTCATCGAGTATTAAGGTACTGAAGGGTCTGGATGCGGTACGC
AAGCGTCCGGGTATGTACAT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MPLSRLMIQQFRNIKACDIRLSAGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRIIQNECSELFVHGRICEHSLS
SDQFELPVGINKQRDGSTEVKIGGQTGQKLAQLAQILPLQLIHPEGFELLTDGPKQRRAFIDWGVFHTEPAFFDAWGRFK
RLSKQRNALLKSAQSYRELSYWDQELARLAEQIDQWRESYVNQLKNVAEQLCRTFLPEFDIDLKYYRGWEKDQPYQSILE
KNFERDQQLGYTFSGPNKADLRIKVNATPVEDVLSRGQLKLMVCALRVAQGQHLTELTGKQCIYLIDDFASELDSLRRQR
LADSLKGTGAQVFVSSITESQVADMLDESSKTFHVAHGVIEQG

Sequences:

>Translated_363_residues
MPLSRLMIQQFRNIKACDIRLSAGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRIIQNECSELFVHGRICEHSLS
SDQFELPVGINKQRDGSTEVKIGGQTGQKLAQLAQILPLQLIHPEGFELLTDGPKQRRAFIDWGVFHTEPAFFDAWGRFK
RLSKQRNALLKSAQSYRELSYWDQELARLAEQIDQWRESYVNQLKNVAEQLCRTFLPEFDIDLKYYRGWEKDQPYQSILE
KNFERDQQLGYTFSGPNKADLRIKVNATPVEDVLSRGQLKLMVCALRVAQGQHLTELTGKQCIYLIDDFASELDSLRRQR
LADSLKGTGAQVFVSSITESQVADMLDESSKTFHVAHGVIEQG
>Mature_362_residues
PLSRLMIQQFRNIKACDIRLSAGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRIIQNECSELFVHGRICEHSLSS
DQFELPVGINKQRDGSTEVKIGGQTGQKLAQLAQILPLQLIHPEGFELLTDGPKQRRAFIDWGVFHTEPAFFDAWGRFKR
LSKQRNALLKSAQSYRELSYWDQELARLAEQIDQWRESYVNQLKNVAEQLCRTFLPEFDIDLKYYRGWEKDQPYQSILEK
NFERDQQLGYTFSGPNKADLRIKVNATPVEDVLSRGQLKLMVCALRVAQGQHLTELTGKQCIYLIDDFASELDSLRRQRL
ADSLKGTGAQVFVSSITESQVADMLDESSKTFHVAHGVIEQG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=360, Percent_Identity=55, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_VIBC3 (A5F493)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F493
- SMR:   A5F493
- STRING:   A5F493
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2505
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   VCHO345073:VC0395_A2505-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41220; Mature: 41089

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRLMIQQFRNIKACDIRLSAGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRI
CCHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IQNECSELFVHGRICEHSLSSDQFELPVGINKQRDGSTEVKIGGQTGQKLAQLAQILPLQ
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCE
LIHPEGFELLTDGPKQRRAFIDWGVFHTEPAFFDAWGRFKRLSKQRNALLKSAQSYRELS
EECCCCCHHHCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWDQELARLAEQIDQWRESYVNQLKNVAEQLCRTFLPEFDIDLKYYRGWEKDQPYQSILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHH
KNFERDQQLGYTFSGPNKADLRIKVNATPVEDVLSRGQLKLMVCALRVAQGQHLTELTGK
HHHHHHHHCCCEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCC
QCIYLIDDFASELDSLRRQRLADSLKGTGAQVFVSSITESQVADMLDESSKTFHVAHGVI
CEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
EQG
CCC
>Mature Secondary Structure 
PLSRLMIQQFRNIKACDIRLSAGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRI
CHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IQNECSELFVHGRICEHSLSSDQFELPVGINKQRDGSTEVKIGGQTGQKLAQLAQILPLQ
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCE
LIHPEGFELLTDGPKQRRAFIDWGVFHTEPAFFDAWGRFKRLSKQRNALLKSAQSYRELS
EECCCCCHHHCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWDQELARLAEQIDQWRESYVNQLKNVAEQLCRTFLPEFDIDLKYYRGWEKDQPYQSILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHH
KNFERDQQLGYTFSGPNKADLRIKVNATPVEDVLSRGQLKLMVCALRVAQGQHLTELTGK
HHHHHHHHCCCEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCC
QCIYLIDDFASELDSLRRQRLADSLKGTGAQVFVSSITESQVADMLDESSKTFHVAHGVI
CEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
EQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA