Click here to switch to the map view.

The map label for this gene is dnaN

Identifier: 227080250

GI number: 227080250

Start: 8873

End: 9973

Strand: Direct

Name: dnaN

Synonym: VCM66_0013

Alternate gene names: 227080250

Gene position: 8873-9973 (Clockwise)

Preceding gene: 227080249

Following gene: 227080251

Centisome position: 0.31

GC content: 49.77

Gene sequence:

>1101_bases
ATGAAATTTACCATTGAACGTAGCCACTTGATTAAACCATTGCAGCAGGTGTCTGGCACTTTAGGTGGTCGCGCCAGCTT
GCCGATCTTGGGTAATTTGTTGCTCAAGGTGGAAGAGAATCAACTGTCGATGACCGCCACCGATTTGGAAGTGGAACTGA
TCAGCCGTGTGACGTTAGAAGGTGAATTTGAAGCAGGCAGTATTACCGTTCCTGCGCGTAAGTTTTTAGATATTTGCCGT
GGTTTGCCCGATAGTGCGGTGATCACTGTGCTGCTTGAAGGCGATCGCATTCAAGTGCGTTCTGGTCGTAGCCGTTTCTC
ACTCGCCACCTTACCTGCCAGTGATTTTCCGAACATTGAAGATTGGCAAAGTGAAGTGCAAGTTTCGCTCACTCAAGCGG
AACTGCGTGGCCTGATTGAGAAAACGCAGTTTTCTATGGCCAACCAAGATGTACGCTACTACTTAAACGGCATGCTGTTT
GAAATTGATGGCACCACCTTGCGTTCTGTGGCGACTGATGGTCACCGTATGGCGGTGGCGCAAGCTCAGCTCGGTGCGGA
TTTTGCGCAAAAGCAGATCATTGTGCCGCGTAAAGGGGTGCTTGAGCTGGTGAAATTGCTTGATGCGCCAGAGCAACCTG
TGGTGCTGCAAATCGGCCACTCTAACTTGCGTGCAGAAGTGAATCACTTTGTGTTTACTTCGAAGCTGGTTGATGGTCGT
TTCCCTGATTATCGCCGTGTATTGCCGCAACATACCAGCAAAACACTACAAACGGGCTGCGAAGAGTTACGCCAAGCATT
TTCGCGTGCCGCGATTCTATCGAATGAAAAATTCCGTGGTGTACGGGTTAATTTGGCTGACAACGGAATGCGCATTACCG
CCAATAACCCAGAGCAAGAAGAAGCGGAAGAGCTACTGGATGTGAGCTTTGAAGGCGAGCCGATTGAGATTGGCTTTAAC
GTCAGCTATATCTTGGATGTGCTTAACACCCTGCGCTGCGACAATGTGCGCGTTTCGATGTCGGATGCCAACGCCAGTGC
CTTGGTGGAGAATGTCGATGATGACAGTGCCATGTACGTGGTCATGCCAATTCGTCTGTAA

Upstream 100 bases:

>100_bases
CAAAGAAGATTATTCGAACCTGATTCGTACGCTTTCCTCTTAATTTGCGCTATGGTTAGCGCACTTGCGTATTTCGCTTT
CACCCGTTTAAGAGAAGACT

Downstream 100 bases:

>100_bases
GGTATAACCGAGTTACCATGCCGCTTTCCCGGTTAATGATCCAACAGTTTCGTAATATTAAAGCCTGTGATATTCGCCTA
TCAGCAGGCTTTAACTTTCT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFTIERSHLIKPLQQVSGTLGGRASLPILGNLLLKVEENQLSMTATDLEVELISRVTLEGEFEAGSITVPARKFLDICR
GLPDSAVITVLLEGDRIQVRSGRSRFSLATLPASDFPNIEDWQSEVQVSLTQAELRGLIEKTQFSMANQDVRYYLNGMLF
EIDGTTLRSVATDGHRMAVAQAQLGADFAQKQIIVPRKGVLELVKLLDAPEQPVVLQIGHSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTSKTLQTGCEELRQAFSRAAILSNEKFRGVRVNLADNGMRITANNPEQEEAEELLDVSFEGEPIEIGFN
VSYILDVLNTLRCDNVRVSMSDANASALVENVDDDSAMYVVMPIRL

Sequences:

>Translated_366_residues
MKFTIERSHLIKPLQQVSGTLGGRASLPILGNLLLKVEENQLSMTATDLEVELISRVTLEGEFEAGSITVPARKFLDICR
GLPDSAVITVLLEGDRIQVRSGRSRFSLATLPASDFPNIEDWQSEVQVSLTQAELRGLIEKTQFSMANQDVRYYLNGMLF
EIDGTTLRSVATDGHRMAVAQAQLGADFAQKQIIVPRKGVLELVKLLDAPEQPVVLQIGHSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTSKTLQTGCEELRQAFSRAAILSNEKFRGVRVNLADNGMRITANNPEQEEAEELLDVSFEGEPIEIGFN
VSYILDVLNTLRCDNVRVSMSDANASALVENVDDDSAMYVVMPIRL
>Mature_366_residues
MKFTIERSHLIKPLQQVSGTLGGRASLPILGNLLLKVEENQLSMTATDLEVELISRVTLEGEFEAGSITVPARKFLDICR
GLPDSAVITVLLEGDRIQVRSGRSRFSLATLPASDFPNIEDWQSEVQVSLTQAELRGLIEKTQFSMANQDVRYYLNGMLF
EIDGTTLRSVATDGHRMAVAQAQLGADFAQKQIIVPRKGVLELVKLLDAPEQPVVLQIGHSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTSKTLQTGCEELRQAFSRAAILSNEKFRGVRVNLADNGMRITANNPEQEEAEELLDVSFEGEPIEIGFN
VSYILDVLNTLRCDNVRVSMSDANASALVENVDDDSAMYVVMPIRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=63.9344262295082, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_VIBCH (Q9KVX5)

Other databases:

- EMBL:   AE003852
- PIR:   F82376
- RefSeq:   NP_062597.1
- ProteinModelPortal:   Q9KVX5
- SMR:   Q9KVX5
- GeneID:   2615703
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0013
- TIGR:   VC_0013
- HOGENOM:   HBG586514
- OMA:   DYNRVIP
- ProtClustDB:   PRK05643
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 40643; Mature: 40643

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIERSHLIKPLQQVSGTLGGRASLPILGNLLLKVEENQLSMTATDLEVELISRVTLE
CEEEECHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEEECCCEEEEEEHHHHEEHHHEEEC
GEFEAGSITVPARKFLDICRGLPDSAVITVLLEGDRIQVRSGRSRFSLATLPASDFPNIE
CCCCCCCEECCHHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCHH
DWQSEVQVSLTQAELRGLIEKTQFSMANQDVRYYLNGMLFEIDGTTLRSVATDGHRMAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCEEEEECCCEEEHHHCCCCEEEEH
QAQLGADFAQKQIIVPRKGVLELVKLLDAPEQPVVLQIGHSNLRAEVNHFVFTSKLVDGR
HHHHCCHHHHCEEECCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHEEEHHHHCCC
FPDYRRVLPQHTSKTLQTGCEELRQAFSRAAILSNEKFRGVRVNLADNGMRITANNPEQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCHH
EAEELLDVSFEGEPIEIGFNVSYILDVLNTLRCDNVRVSMSDANASALVENVDDDSAMYV
HHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEECC
>Mature Secondary Structure
MKFTIERSHLIKPLQQVSGTLGGRASLPILGNLLLKVEENQLSMTATDLEVELISRVTLE
CEEEECHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEEECCCEEEEEEHHHHEEHHHEEEC
GEFEAGSITVPARKFLDICRGLPDSAVITVLLEGDRIQVRSGRSRFSLATLPASDFPNIE
CCCCCCCEECCHHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCHH
DWQSEVQVSLTQAELRGLIEKTQFSMANQDVRYYLNGMLFEIDGTTLRSVATDGHRMAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCEEEEECCCEEEHHHCCCCEEEEH
QAQLGADFAQKQIIVPRKGVLELVKLLDAPEQPVVLQIGHSNLRAEVNHFVFTSKLVDGR
HHHHCCHHHHCEEECCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHEEEHHHHCCC
FPDYRRVLPQHTSKTLQTGCEELRQAFSRAAILSNEKFRGVRVNLADNGMRITANNPEQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCHH
EAEELLDVSFEGEPIEIGFNVSYILDVLNTLRCDNVRVSMSDANASALVENVDDDSAMYV
HHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301