Click here to switch to the map view.

The map label for this gene is gyrB

Identifier: 227080252

GI number: 227080252

Start: 11103

End: 13520

Strand: Direct

Name: gyrB

Synonym: VCM66_0015

Alternate gene names: 227080252

Gene position: 11103-13520 (Clockwise)

Preceding gene: 227080251

Following gene: 227080253

Centisome position: 0.38

GC content: 50.5

Gene sequence:

>2418_bases
ATGTCGAACAATTACGATTCATCGAGTATTAAGGTACTGAAGGGTCTGGATGCGGTACGCAAGCGTCCGGGTATGTACAT
CGGCGACACTGATGATGGCACCGGTCTGCACCACATGGTTTTTGAGGTGGTGGATAACTCAATTGATGAAGCGTTGGCAG
GTTACTGTAAAGACATCGTGGTCACCATCCACGAAGACAACTCAGTGTCAGTCAGCGATGACGGTCGTGGTATCCCGACC
GAAATGCATCCTGAAGAGAAAGTGTCTGCGGCAGAAGTCATCATGACTGTACTGCACGCCGGCGGTAAATTTGACGACAA
CTCCTATAAAGTCTCCGGTGGTCTGCACGGGGTAGGTGTGTCGGTGGTTAACGCGCTTTCTGAAAAAGTGCTGCTTACCA
TTTATCGTGGTGGCAAGATCCATTCGCAAACTTACCATCATGGTGTGCCACAAGCACCGTTGGCTGTGGTAGGTGAGACA
GAGCGTACCGGTACTACCGTACGTTTCTGGCCTAGTGCACAGACTTTTACCAATATCGAATTCCATTACGACATTTTGGC
TAAACGCCTGCGTGAGCTGTCATTCCTGAACTCTGGCGTGTCGATCAAGCTGACCGATGAACGTGAAGAAGATAAAAAAG
ACCACTTCATGTATGAAGGGGGTATTCAAGCGTTTGTGACCCACTTGAACCGTAACAAAACGCCAATCCATGAGAAAGTC
TTCCACTTTAACCAAGAGCGTGAAGATGGCATCAGCGTGGAAGTGGCAATGCAGTGGAACGATGGTTTCCAAGAAAACAT
CTACTGCTTTACTAACAACATCCCACAGCGTGATGGTGGTACCCACTTAGCCGGTTTCCGTGGTGCGTTGACCCGTACTT
TGAACAACTACATGGACAAAGAAGGCTTCTCGAAGAAAGCGCAAGCGGCAACCTCGGGTGATGATGCGCGTGAAGGCTTA
ACGGCAGTGGTTTCGGTGAAAGTGCCGGATCCTAAATTCTCAAGCCAAACCAAAGATAAGCTGGTTTCTTCGGAAGTAAA
ATCCGCGGTTGAGTCAGCCATGAATGAGAAGCTGGCGGATTTCCTAGCGGAAAACCCAAGCGAAGCGAAAAACGTTTGTT
CGAAGATTATTGATGCGGCGCGTGCGCGTGAAGCGGCGCGTAAAGCCCGGGAAATGACTCGCCGTAAAGGCGCGCTGGAT
CTTGCTGGCTTACCCGGCAAACTGGCGGATTGTCAGGAAAAAGACCCAGCATTGTCTGAACTCTACATAGTGGAGGGTGA
CTCAGCGGGCGGTTCAGCCAAACAAGGCCGTAACCGTAAGAATCAAGCGATTCTGCCGCTGAAAGGTAAAATCCTCAACG
TAGAAAAAGCGCGTTTTGACAAGATGTTGTCTTCACAAGAAGTCGCGACCCTCATCACTGCATTAGGCTGTGGTATCGGC
CGTGATGAGTACAACCCAGATAAACTGCGTTATCACAACATCATCATCATGACCGATGCGGACGTCGACGGCTCGCACAT
TCGTACTCTGCTGTTGACCTTTTTCTACCGCCAAATGCCTGAGTTGATTGAGCGTGGTTACATCTACATTGCTCAGCCAC
CACTGTATAAAGTGAAGAAAGGCAAACAAGAGCAGTACATCAAAGATGAAGAGGCGATGAACCAGTACCAAGTGGCACTG
GCGATGGATGGTGCAGAGCTGCACGTGAATGCCGATGCGCCAGCGTTAGCGGGTGAACCGCTGGAGAAGCTGGTTCAGCA
ATATAACGCCGCGATCAAGCTGGTTGAGCGCATGAGCCGCCGTTACCCTTACGCGATGCTGCATGAGCTGATTTACGTAC
CACGCATCAATGCCGAGCTGTGTGCGGATAAAGCCGCGGTTGAAGCTTGGACACAGCGTTTAGTCGAACAGTTGAATGCT
AAAGAAGTGGGTGCGAGCCAATACAGCGTTCTGGTTGAACACAATGCTGAGCTGAATGTGTACTTGCCAAAAATTCAGGT
GCGTACTCACGGTGTGACTCATGAATACTTACTGAGCGCGGATCTGATCAACTCGAAAGAGTACGCTAAGTTAGCCGATC
TGTCGGAAGCGCTGGACGGTTTGATTGAAGCGGGAGCGTTTATCAAACGTGGTGAGCGAGTACAACCGATCAGCAGCTTT
GCCGCCGCGCTGGATTGGCTGATCAAAGAGTCGCGCCGCGGTCTGTCTATCCAACGCTATAAAGGTCTGGGCGAAATGAA
CCCTGATCAGCTGTGGGAAACTACGATGGATCCAGAAACGCGTCGTATGATGCAAGTCACTATCGAAGATGCGGTAGGCG
CGGACGAACTGTTCACGACGCTCATGGGCGATCAAGTTGAACCACGTCGTGCATTTATCGAAACCAACGCACTGAAAGTG
GCCAACCTCGACGTATAA

Upstream 100 bases:

>100_bases
CCGAAAGCCAAGTGGCGGACATGTTAGATGAGTCCAGTAAAACATTTCACGTTGCCCACGGTGTAATAGAGCAAGGATAA
ACAGAGTGAGAGAGTAACTC

Downstream 100 bases:

>100_bases
GCGTTTGGTTGATTAACAATCTGCGATAAAAAAGCAGCGCTGTGGCGCTGCTTTTTTTATCTCTGCATTTTGTCCCTTCC
CTCTGTTGGATGACGGCAGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGYCKDIVVTIHEDNSVSVSDDGRGIPT
EMHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVLLTIYRGGKIHSQTYHHGVPQAPLAVVGET
ERTGTTVRFWPSAQTFTNIEFHYDILAKRLRELSFLNSGVSIKLTDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
FHFNQEREDGISVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRTLNNYMDKEGFSKKAQAATSGDDAREGL
TAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLADFLAENPSEAKNVCSKIIDAARAREAARKAREMTRRKGALD
LAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEEAMNQYQVAL
AMDGAELHVNADAPALAGEPLEKLVQQYNAAIKLVERMSRRYPYAMLHELIYVPRINAELCADKAAVEAWTQRLVEQLNA
KEVGASQYSVLVEHNAELNVYLPKIQVRTHGVTHEYLLSADLINSKEYAKLADLSEALDGLIEAGAFIKRGERVQPISSF
AAALDWLIKESRRGLSIQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADELFTTLMGDQVEPRRAFIETNALKV
ANLDV

Sequences:

>Translated_805_residues
MSNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGYCKDIVVTIHEDNSVSVSDDGRGIPT
EMHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVLLTIYRGGKIHSQTYHHGVPQAPLAVVGET
ERTGTTVRFWPSAQTFTNIEFHYDILAKRLRELSFLNSGVSIKLTDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
FHFNQEREDGISVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRTLNNYMDKEGFSKKAQAATSGDDAREGL
TAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLADFLAENPSEAKNVCSKIIDAARAREAARKAREMTRRKGALD
LAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEEAMNQYQVAL
AMDGAELHVNADAPALAGEPLEKLVQQYNAAIKLVERMSRRYPYAMLHELIYVPRINAELCADKAAVEAWTQRLVEQLNA
KEVGASQYSVLVEHNAELNVYLPKIQVRTHGVTHEYLLSADLINSKEYAKLADLSEALDGLIEAGAFIKRGERVQPISSF
AAALDWLIKESRRGLSIQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADELFTTLMGDQVEPRRAFIETNALKV
ANLDV
>Mature_804_residues
SNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGYCKDIVVTIHEDNSVSVSDDGRGIPTE
MHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVLLTIYRGGKIHSQTYHHGVPQAPLAVVGETE
RTGTTVRFWPSAQTFTNIEFHYDILAKRLRELSFLNSGVSIKLTDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKVF
HFNQEREDGISVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRTLNNYMDKEGFSKKAQAATSGDDAREGLT
AVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLADFLAENPSEAKNVCSKIIDAARAREAARKAREMTRRKGALDL
AGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEEAMNQYQVALA
MDGAELHVNADAPALAGEPLEKLVQQYNAAIKLVERMSRRYPYAMLHELIYVPRINAELCADKAAVEAWTQRLVEQLNAK
EVGASQYSVLVEHNAELNVYLPKIQVRTHGVTHEYLLSADLINSKEYAKLADLSEALDGLIEAGAFIKRGERVQPISSFA
AALDWLIKESRRGLSIQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADELFTTLMGDQVEPRRAFIETNALKVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI19913408, Length=550, Percent_Identity=25.4545454545455, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913406, Length=565, Percent_Identity=26.0176991150442, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI48994957, Length=806, Percent_Identity=77.9156327543424, Blast_Score=1286, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=556, Percent_Identity=39.2086330935252, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=541, Percent_Identity=25.6931608133087, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=528, Percent_Identity=24.0530303030303, Blast_Score=126, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=594, Percent_Identity=25.2525252525253, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=534, Percent_Identity=26.0299625468165, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_VIBCH (Q9KVX3)

Other databases:

- EMBL:   AE003852
- PIR:   H82376
- RefSeq:   NP_062599.1
- ProteinModelPortal:   Q9KVX3
- GeneID:   2615137
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0015
- TIGR:   VC_0015
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK14939
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 89521; Mature: 89389

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGYCKDIV
CCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEE
VTIHEDNSVSVSDDGRGIPTEMHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKVLLTIYRGGKIHSQTYHHGVPQAPLAVVGETERTGTTVRFWPSAQTFTNIE
HHHHHHHHCEEEEEECCCEEECCHHHCCCCCCCEEEECCCCCCCCEEEECCCCCCEEEEE
FHYDILAKRLRELSFLNSGVSIKLTDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
EHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEECCCHHHHHHHHCCCCCCHHHHH
FHFNQEREDGISVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRTLNNYMDK
HHCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EGFSKKAQAATSGDDAREGLTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLAD
CCCCHHHHHCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLAENPSEAKNVCSKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQEKDPALSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEE
LYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKK
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDEEAMNQYQVALAMDGAELHVNADAPALAGEPLEKLVQQYNAAIKLVERMSR
CCHHHHCCCHHHHHHEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPYAMLHELIYVPRINAELCADKAAVEAWTQRLVEQLNAKEVGASQYSVLVEHNAELNV
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEEEEEECCCEEEE
YLPKIQVRTHGVTHEYLLSADLINSKEYAKLADLSEALDGLIEAGAFIKRGERVQPISSF
EEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AAALDWLIKESRRGLSIQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADELFTT
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
LMGDQVEPRRAFIETNALKVANLDV
HHCCCCCHHHHHHCCCCEEEEECCC
>Mature Secondary Structure 
SNNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGYCKDIV
CCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEE
VTIHEDNSVSVSDDGRGIPTEMHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKVLLTIYRGGKIHSQTYHHGVPQAPLAVVGETERTGTTVRFWPSAQTFTNIE
HHHHHHHHCEEEEEECCCEEECCHHHCCCCCCCEEEECCCCCCCCEEEECCCCCCEEEEE
FHYDILAKRLRELSFLNSGVSIKLTDEREEDKKDHFMYEGGIQAFVTHLNRNKTPIHEKV
EHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEECCCHHHHHHHHCCCCCCHHHHH
FHFNQEREDGISVEVAMQWNDGFQENIYCFTNNIPQRDGGTHLAGFRGALTRTLNNYMDK
HHCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EGFSKKAQAATSGDDAREGLTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLAD
CCCCHHHHHCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLAENPSEAKNVCSKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQEKDPALSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEE
LYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKK
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDEEAMNQYQVALAMDGAELHVNADAPALAGEPLEKLVQQYNAAIKLVERMSR
CCHHHHCCCHHHHHHEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPYAMLHELIYVPRINAELCADKAAVEAWTQRLVEQLNAKEVGASQYSVLVEHNAELNV
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEEEEEECCCEEEE
YLPKIQVRTHGVTHEYLLSADLINSKEYAKLADLSEALDGLIEAGAFIKRGERVQPISSF
EEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AAALDWLIKESRRGLSIQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADELFTT
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
LMGDQVEPRRAFIETNALKVANLDV
HHCCCCCHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301