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The map label for this gene is adh1 [H]

Identifier: 226949408

GI number: 226949408

Start: 2406725

End: 2407867

Strand: Reverse

Name: adh1 [H]

Synonym: CLM_2333

Alternate gene names: 226949408

Gene position: 2407867-2406725 (Counterclockwise)

Preceding gene: 226949410

Following gene: 226949407

Centisome position: 57.95

GC content: 34.47

Gene sequence:

>1143_bases
ATGAAAGAATTTAGTATTAATACAGATGTCTATTTTGGAGAAGGATCTTTAGATAGACTAAAGGAAATTAAAAATAAAAG
AGTACTAATTGTATGTGATAAATTTATGGAAACTTCAGGTATGGCTCAAAAAGTAGAACAAAAACTTGTTGATTGCAAAG
TAGCTATATATAGCGATATTGTACCTGATCCATCTGTAGAAGTTATTGCATCTGGTATTCAAAAACTTCAAAGTTGCAAT
GCAGAGATTATGATAGCTCTTGGTGGAGGTTCATCAATTGATGGAGCTAAGGCCATGAGAGAATATGCCAAAAAAATTAC
TGGAGGAGTTTCCACTATAGAAGAGTTTTATGCTATACCTACTACAAGTGGTACAGGCTCTGAAGTAACTGAATATGCAG
TAATTACAAACAAACAAGAAGGATTAAAGTATGCTCTTACAGACAAATCACTTCTTCCTACGGTTGCTATTCTTGACCCT
GAGTTGGTTAAATCTGTACCCAAAGCTATAACTGCGGATACAGGAATGGATGTAATCACACATGCCATGGAGGCCTATGT
TTCAAAAAATGCTACTGATTTTTCAGATGCTCTTGCAGAAAAAGCCTTTACTTTAGCTTTTAGATTTTTACCACAGGCTT
ATGCTGATGGGAATGATATTATAGCAAGAGAAAAACTTCACAATGCATCATGCCTTGCTGGTATGGCTTTTAATGCAGCG
GGACTTGGAATTACTCATAGTTTAGCTCATGCAGTAGGTGGGAAGTTACATATTTCTCATGGAAGAAGTAATGCAATAAT
ACTTCCTTACGTAGTTGAATATAACGCTAGTTTAAATAAAGAAACCTTTAATGTTGAATATGACGTAGCAGCTAAGAAAT
ATCAAAGGCTTGCTAAGTTATTGAAGTTGCATGCGCCAAATGTAAATATTGGTGTTAATAATCTAATTAAAAGTATTGTA
GAACTTCAAAATACATTAATGATTCCAGCCACATTAAAAGAACAAGGCGCTGATATGGCTTTGGCTGAAGCGTCAAGAGA
AGAGATTATTAATGCAGCTCTTAAGGATGTGTGTACAACAGCAAATCCTAGAGAAGCTACAAAGGAAGATTTATCAAAAA
TTTTAGAAAAAGTTTTAGGATAG

Upstream 100 bases:

>100_bases
ACTTAGTTTGATTGTTATAATTGTAGGTTAATTTTATATTTAAGTTAAAGAATACCAAATTCATTCATATCTTGATATTT
TTTTATAGGAGAAAATGCAT

Downstream 100 bases:

>100_bases
ACTATTTTATACCACTCAGTAATGGGTGGTATTTTGCTCAAATTTTCAGAAAAATTGAAAAATTAAATAGAATTTATAAT
ATAAAAAAATAAGGGGGATT

Product: iron-containing alcohol dehydrogenase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: BDH [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAQKVEQKLVDCKVAIYSDIVPDPSVEVIASGIQKLQSCN
AEIMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIPTTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDP
ELVKSVPKAITADTGMDVITHAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
GLGITHSLAHAVGGKLHISHGRSNAIILPYVVEYNASLNKETFNVEYDVAAKKYQRLAKLLKLHAPNVNIGVNNLIKSIV
ELQNTLMIPATLKEQGADMALAEASREEIINAALKDVCTTANPREATKEDLSKILEKVLG

Sequences:

>Translated_380_residues
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAQKVEQKLVDCKVAIYSDIVPDPSVEVIASGIQKLQSCN
AEIMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIPTTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDP
ELVKSVPKAITADTGMDVITHAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
GLGITHSLAHAVGGKLHISHGRSNAIILPYVVEYNASLNKETFNVEYDVAAKKYQRLAKLLKLHAPNVNIGVNNLIKSIV
ELQNTLMIPATLKEQGADMALAEASREEIINAALKDVCTTANPREATKEDLSKILEKVLG
>Mature_380_residues
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAQKVEQKLVDCKVAIYSDIVPDPSVEVIASGIQKLQSCN
AEIMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIPTTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDP
ELVKSVPKAITADTGMDVITHAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
GLGITHSLAHAVGGKLHISHGRSNAIILPYVVEYNASLNKETFNVEYDVAAKKYQRLAKLLKLHAPNVNIGVNNLIKSIV
ELQNTLMIPATLKEQGADMALAEASREEIINAALKDVCTTANPREATKEDLSKILEKVLG

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=311, Percent_Identity=24.7588424437299, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787493, Length=399, Percent_Identity=36.3408521303258, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082107, Length=389, Percent_Identity=33.1619537275064, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI48994951, Length=378, Percent_Identity=34.6560846560847, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789163, Length=388, Percent_Identity=32.7319587628866, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1789386, Length=290, Percent_Identity=26.8965517241379, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=423, Percent_Identity=24.3498817966903, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321181, Length=390, Percent_Identity=36.6666666666667, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24657991, Length=372, Percent_Identity=23.1182795698925, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1

Molecular weight: Translated: 41021; Mature: 41021

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAQKVEQKLVDCKVAIYSDI
CCCEECCCCEEECCCCHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHCC
VPDPSVEVIASGIQKLQSCNAEIMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIP
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
TTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDPELVKSVPKAITADTGMDVIT
CCCCCCCHHHHEEEEECCCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHH
HAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHCCC
GLGITHSLAHAVGGKLHISHGRSNAIILPYVVEYNASLNKETFNVEYDVAAKKYQRLAKL
CCCHHHHHHHHCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHH
LKLHAPNVNIGVNNLIKSIVELQNTLMIPATLKEQGADMALAEASREEIINAALKDVCTT
HHHCCCCCCCCHHHHHHHHHHHHHCEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
ANPREATKEDLSKILEKVLG
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAQKVEQKLVDCKVAIYSDI
CCCEECCCCEEECCCCHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHCC
VPDPSVEVIASGIQKLQSCNAEIMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIP
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
TTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDPELVKSVPKAITADTGMDVIT
CCCCCCCHHHHEEEEECCCCCCEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHH
HAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHCCC
GLGITHSLAHAVGGKLHISHGRSNAIILPYVVEYNASLNKETFNVEYDVAAKKYQRLAKL
CCCHHHHHHHHCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHH
LKLHAPNVNIGVNNLIKSIVELQNTLMIPATLKEQGADMALAEASREEIINAALKDVCTT
HHHCCCCCCCCHHHHHHHHHHHHHCEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
ANPREATKEDLSKILEKVLG
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]