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The map label for this gene is nuoF2 [H]

Identifier: 226949092

GI number: 226949092

Start: 2075926

End: 2077821

Strand: Reverse

Name: nuoF2 [H]

Synonym: CLM_2008

Alternate gene names: 226949092

Gene position: 2077821-2075926 (Counterclockwise)

Preceding gene: 226949093

Following gene: 226949091

Centisome position: 50.0

GC content: 32.44

Gene sequence:

>1896_bases
ATGGAGAAAATTAATTCTTATAAAGAATTGAAAAGCTATTATGAAAACTATAAGGATTTATTAAAAAGTAGACATACCAC
CCATGAAGAAGAAACAGCAGTAGAAAATAAAAAATGTGAAAGACTTATATTAGTTTGTGGCGGTACAGGATGTAAATCAG
CTGACAGTTATAAAATTGTAGAAAATTTAAAAGAAGAAATAAATAAATTAGGACTACAAGAAGAAGTAAAAGTTTCTATC
ACAGGATGCTTTGGTTTCTGTGAAAAAGGTCCTATAGTTAAAATAAATCCAGATAATGTTTTTTATGTTAAAGTAACACC
AGAAGATGCTAAGGAAATAGCAGAAAAGCATTTATTAAAAGGTGAAGTAGTAGAAAGATTACTTTATGAAGAACCAACCC
TTAAAGAAAAAGTAAAAAGACAAGATGAAATGTCTTTTTACAAAAAACAAAAAAGAATTGCTCTTAGAAATTGTGGCCTT
ATAAATCCAGAAGATATAAAAGAATGTATCGGCTCAGAGGGATACTTAGCCCTAGGAAAAGTTTTAAGTGAAATGACACC
TGATGAATTAATAAAATTAATAACTGACTCAGGCCTTAGAGGAAGAGGCGGCGGTGGCTTCTCTACAGGTAAAAAATGGA
GTTTTGGAAAAATGTATGATAGTGATGTTAAATATATAATATGTAATGCAGATGAAGGTGATCCAGGAGCCTTTATGGAT
CGTTCCATATTAGAAGGAGATCCTCATAGCATAATTGAAGCTATGGCTATAGCTGGCTATGCCATAGGAGCTTCCAAAGG
TCGCATATATATAAGAGCAGAATATCCTTTAGCTGTAAATAGATTAAAAATAGCTATGGATCAAGCTAAAGAGTATGGTC
TTTTAGGAGAAAATATTTTAGGCACCGGTTTTAACTTTAATATAGAAATAAAATATGGTGCAGGAGCCTTCGTATGTGGG
GAAGAGACAGCATTAATACACTCCATAGAAGGAGAAAGGGGAGAACCTACTTATAAACCACCATTCCCAGCAGAAGCTGG
TCTTTGGAACAAGCCAACAGTAGTAAATAATGTAGAAACTTTAGCAAATATACCTGCCATAATAAATAAGGGGGCAGATT
GGTATAAATCCATAGGAACAGAAAAATCAAATGGTACAAAAGTTTTCGCCCTAGCAGGGAAAATAAATAATGTTGGATTA
GTTGAAGTTCCTATGGGAACTACTCTAAGAGAGATAATATATGATATAGGTGGAGGTATAAAAAACGGCAAGAAATTTAA
AGCTGTTCAAACAGGAGGACCTTCAGGTGGATGTATTCCAGCTTCACTTTTAGATATTCCTATAGACTATGAATCATTAA
CATCTATAGGATCTATGATGGGTTCCGGTGGAATGATTGTTATGGATGAAGATAATTGTATGGTAGATATAGCAAAATTT
TATCTTGAATTTACAGTGGATGAATCCTGTGGTAAATGTACCCCATGTAGAATTGGTAATAAGAGACTTTTAGAAACTTT
AATAAAGATAACCAATGGGAAAGGTTCAGAAGAAGATTTAAATAAATTAGATGAATTATCACATATAATAAAGGATACTT
CCTTATGTGGATTAGGACAAACTGCACCAAATCCAGTACTAAGTACTATGAGATATTTTATGGATGAATACGAAGCTCAT
GTTAATGAAAAAAGATGTCCATCAGGTACTTGTAAAAATTTACTTCATTATGAGATTACTGATAAATGTATCGGATGTAC
TAAATGTGCACGAGGTTGCCCAGTATCCTGTATAATAGGAAAAGTTAAAGAAAAACATTTTATAAATCAAGAAAAATGTA
TTAAATGTGGAAATTGTTATAGTGCTTGTCCAGTTGGAGCTATTATAAAGAAATAG

Upstream 100 bases:

>100_bases
TAGCTCCAGTGGTTACAATTAATGATAAAGTATATGGACATTTTACTAAAAATGAAGTAGCTAAAGTATTGGAAGAATAT
GGGGCGTAGGAGGGATATTT

Downstream 100 bases:

>100_bases
GAAGAAAGAGGTGAATAAGATGAGTTTAGTAACTTTAAATATAAATGGCAAAGAGCTTAAGGTAGAGAAGGGAACTACTA
TATTAGATGCAGCAAAACTT

Product: putative [Fe] hydrogenase, electron-transfer subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSYKIVENLKEEINKLGLQEEVKVSI
TGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLKGEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGL
INPEDIKECIGSEGYLALGKVLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
RSILEGDPHSIIEAMAIAGYAIGASKGRIYIRAEYPLAVNRLKIAMDQAKEYGLLGENILGTGFNFNIEIKYGAGAFVCG
EETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVETLANIPAIINKGADWYKSIGTEKSNGTKVFALAGKINNVGL
VEVPMGTTLREIIYDIGGGIKNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTSIGSMMGSGGMIVMDEDNCMVDIAKF
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQTAPNPVLSTMRYFMDEYEAH
VNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIGKVKEKHFINQEKCIKCGNCYSACPVGAIIKK

Sequences:

>Translated_631_residues
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSYKIVENLKEEINKLGLQEEVKVSI
TGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLKGEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGL
INPEDIKECIGSEGYLALGKVLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
RSILEGDPHSIIEAMAIAGYAIGASKGRIYIRAEYPLAVNRLKIAMDQAKEYGLLGENILGTGFNFNIEIKYGAGAFVCG
EETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVETLANIPAIINKGADWYKSIGTEKSNGTKVFALAGKINNVGL
VEVPMGTTLREIIYDIGGGIKNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTSIGSMMGSGGMIVMDEDNCMVDIAKF
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQTAPNPVLSTMRYFMDEYEAH
VNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIGKVKEKHFINQEKCIKCGNCYSACPVGAIIKK
>Mature_631_residues
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSYKIVENLKEEINKLGLQEEVKVSI
TGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLKGEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGL
INPEDIKECIGSEGYLALGKVLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
RSILEGDPHSIIEAMAIAGYAIGASKGRIYIRAEYPLAVNRLKIAMDQAKEYGLLGENILGTGFNFNIEIKYGAGAFVCG
EETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVETLANIPAIINKGADWYKSIGTEKSNGTKVFALAGKINNVGL
VEVPMGTTLREIIYDIGGGIKNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTSIGSMMGSGGMIVMDEDNCMVDIAKF
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQTAPNPVLSTMRYFMDEYEAH
VNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIGKVKEKHFINQEKCIKCGNCYSACPVGAIIKK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=380, Percent_Identity=40.7894736842105, Blast_Score=296, Evalue=4e-80,
Organism=Homo sapiens, GI20149568, Length=380, Percent_Identity=40.7894736842105, Blast_Score=296, Evalue=4e-80,
Organism=Escherichia coli, GI1788620, Length=404, Percent_Identity=42.3267326732673, Blast_Score=352, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17531727, Length=380, Percent_Identity=41.5789473684211, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI19920794, Length=378, Percent_Identity=39.4179894179894, Blast_Score=286, Evalue=4e-77,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=39.1534391534392, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24653819, Length=382, Percent_Identity=33.2460732984293, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24653817, Length=353, Percent_Identity=31.728045325779, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 69345; Mature: 69345

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSYKIV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
ENLKEEINKLGLQEEVKVSITGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLK
HHHHHHHHHCCCCCEEEEEEEEEEHHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHH
GEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGLINPEDIKECIGSEGYLALGK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
VLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHC
RSILEGDPHSIIEAMAIAGYAIGASKGRIYIRAEYPLAVNRLKIAMDQAKEYGLLGENIL
CHHHCCCHHHHHHHHHHHHHEEECCCCEEEEEECCCEEHHHHHHHHHHHHHCCCCCCCCC
GTGFNFNIEIKYGAGAFVCGEETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVET
CCCEEEEEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHH
LANIPAIINKGADWYKSIGTEKSNGTKVFALAGKINNVGLVEVPMGTTLREIIYDIGGGI
HHHCHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCC
KNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTSIGSMMGSGGMIVMDEDNCMVDIAKF
CCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQ
HHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
TAPNPVLSTMRYFMDEYEAHVNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KVKEKHFINQEKCIKCGNCYSACPVGAIIKK
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MEKINSYKELKSYYENYKDLLKSRHTTHEEETAVENKKCERLILVCGGTGCKSADSYKIV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
ENLKEEINKLGLQEEVKVSITGCFGFCEKGPIVKINPDNVFYVKVTPEDAKEIAEKHLLK
HHHHHHHHHCCCCCEEEEEEEEEEHHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHH
GEVVERLLYEEPTLKEKVKRQDEMSFYKKQKRIALRNCGLINPEDIKECIGSEGYLALGK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
VLSEMTPDELIKLITDSGLRGRGGGGFSTGKKWSFGKMYDSDVKYIICNADEGDPGAFMD
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHC
RSILEGDPHSIIEAMAIAGYAIGASKGRIYIRAEYPLAVNRLKIAMDQAKEYGLLGENIL
CHHHCCCHHHHHHHHHHHHHEEECCCCEEEEEECCCEEHHHHHHHHHHHHHCCCCCCCCC
GTGFNFNIEIKYGAGAFVCGEETALIHSIEGERGEPTYKPPFPAEAGLWNKPTVVNNVET
CCCEEEEEEEEECCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHH
LANIPAIINKGADWYKSIGTEKSNGTKVFALAGKINNVGLVEVPMGTTLREIIYDIGGGI
HHHCHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCC
KNGKKFKAVQTGGPSGGCIPASLLDIPIDYESLTSIGSMMGSGGMIVMDEDNCMVDIAKF
CCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
YLEFTVDESCGKCTPCRIGNKRLLETLIKITNGKGSEEDLNKLDELSHIIKDTSLCGLGQ
HHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
TAPNPVLSTMRYFMDEYEAHVNEKRCPSGTCKNLLHYEITDKCIGCTKCARGCPVSCIIG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KVKEKHFINQEKCIKCGNCYSACPVGAIIKK
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]