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The map label for this gene is nuoE [H]

Identifier: 226949093

GI number: 226949093

Start: 2077833

End: 2078312

Strand: Reverse

Name: nuoE [H]

Synonym: CLM_2009

Alternate gene names: 226949093

Gene position: 2078312-2077833 (Counterclockwise)

Preceding gene: 226949094

Following gene: 226949092

Centisome position: 50.02

GC content: 33.12

Gene sequence:

>480_bases
ATGTGTTCCAATGAGTTAGCAAATACCAAATTCAAAGAACTAGAAGAATATATAAACAATATCTCCAATAAGAAAGGATC
CCTTATAGAAGTGCTTCATAAGGCTCAACATATTTTTGGATATCTTCCAAATGAAGTGCAGGAATTTGTAGCTAAAAAGC
TAGATATTCCCGTTTCAAAGGTTTATGGAGTTATTACTTTTTACTCTTATTTTACTACAGAACCAAAGGGTGAAAATGTT
ATAAATGTCTGTATGGGCACAGCTTGCTTTGTAAAGGGAGCTGGGGATGTACTTTCAGAATTTGAAAAGAAATTAAATAT
AAAAGTTGGAGAAACCACTAAAGATGGTAAATTCACATTACAAGTTTTGAGATGTGTAGGGGCCTGTGGATTAGCTCCAG
TGGTTACAATTAATGATAAAGTATATGGACATTTTACTAAAAATGAAGTAGCTAAAGTATTGGAAGAATATGGGGCGTAG

Upstream 100 bases:

>100_bases
TAATTTTCAGAAATATGTTAATTTTCTAAAGAATCTATAGTATAATATAAAATAAAAAAATAAATGGATTTTTCAAATAG
CAAAAAGGGGGAGGATTCCT

Downstream 100 bases:

>100_bases
GAGGGATATTTATGGAGAAAATTAATTCTTATAAAGAATTGAAAAGCTATTATGAAAACTATAAGGATTTATTAAAAAGT
AGACATACCACCCATGAAGA

Product: putative Fe] hydrogenase, electron-transfer subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MCSNELANTKFKELEEYINNISNKKGSLIEVLHKAQHIFGYLPNEVQEFVAKKLDIPVSKVYGVITFYSYFTTEPKGENV
INVCMGTACFVKGAGDVLSEFEKKLNIKVGETTKDGKFTLQVLRCVGACGLAPVVTINDKVYGHFTKNEVAKVLEEYGA

Sequences:

>Translated_159_residues
MCSNELANTKFKELEEYINNISNKKGSLIEVLHKAQHIFGYLPNEVQEFVAKKLDIPVSKVYGVITFYSYFTTEPKGENV
INVCMGTACFVKGAGDVLSEFEKKLNIKVGETTKDGKFTLQVLRCVGACGLAPVVTINDKVYGHFTKNEVAKVLEEYGA
>Mature_159_residues
MCSNELANTKFKELEEYINNISNKKGSLIEVLHKAQHIFGYLPNEVQEFVAKKLDIPVSKVYGVITFYSYFTTEPKGENV
INVCMGTACFVKGAGDVLSEFEKKLNIKVGETTKDGKFTLQVLRCVGACGLAPVVTINDKVYGHFTKNEVAKVLEEYGA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=151, Percent_Identity=37.7483443708609, Blast_Score=108, Evalue=3e-24,
Organism=Escherichia coli, GI1788621, Length=153, Percent_Identity=34.640522875817, Blast_Score=105, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17561328, Length=161, Percent_Identity=40.3726708074534, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859877, Length=137, Percent_Identity=40.8759124087591, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355353, Length=132, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17639; Mature: 17639

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSNELANTKFKELEEYINNISNKKGSLIEVLHKAQHIFGYLPNEVQEFVAKKLDIPVSK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
VYGVITFYSYFTTEPKGENVINVCMGTACFVKGAGDVLSEFEKKLNIKVGETTKDGKFTL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCCHHHH
QVLRCVGACGLAPVVTINDKVYGHFTKNEVAKVLEEYGA
HHHHHHHCCCCCCEEEECCCEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MCSNELANTKFKELEEYINNISNKKGSLIEVLHKAQHIFGYLPNEVQEFVAKKLDIPVSK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
VYGVITFYSYFTTEPKGENVINVCMGTACFVKGAGDVLSEFEKKLNIKVGETTKDGKFTL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCCHHHH
QVLRCVGACGLAPVVTINDKVYGHFTKNEVAKVLEEYGA
HHHHHHHCCCCCCEEEECCCEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]