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The map label for this gene is sgcA [H]

Identifier: 226225347

GI number: 226225347

Start: 2851491

End: 2851937

Strand: Reverse

Name: sgcA [H]

Synonym: Lm4b_02770

Alternate gene names: 226225347

Gene position: 2851937-2851491 (Counterclockwise)

Preceding gene: 226225348

Following gene: 226225346

Centisome position: 97.91

GC content: 42.95

Gene sequence:

>447_bases
ATGTTATTAGACCGAGTTACTTTAGAAGATATTGAGGTAAATTTAAAAGCAAAAGACTGGCGCGAAGCCATCCAAACATC
AGCAAGACTTTTACTAGAACGAGGCGCTATCAAAGAGAGTTATATTGACGGAATGATTCAAAGTGTAGAAAATAATGGAC
CATATATAGTGATTGCTAAACATATCGCCCTAGCGCACACACGACCAGAATTCGGCGTCATTCGCGGTGGCCTAACTTTT
GCAACACTGGCTGACGGCGTGGCTTTTGGAAGCGACATGTTCGACCCGGTGAAACTAGTTATTACCCTTGCGGCAACAGA
CTCAGAAAGCCATTTAGAAGTGTTGTCAGAGCTTGCAGAAGTGCTCATGGACGAGGAAAAGGTAACCCGTTTGATCGAAG
CAAATTCAAGCCAAGCGTTTTACGATGAGTTAACAAAAGAAGGATAA

Upstream 100 bases:

>100_bases
ACATAGTGATTTAACCCCGAACTATTTGGAGCGAGAGTCGCTGGCGGAGGTAGATTTAACAGAAATAATGGCTGAATATA
ATAAATAAGGGGGCGCGAGC

Downstream 100 bases:

>100_bases
TAATTTCACTAAAGAGGTGAAGAAATGGAAAATTATCTTTGTGTCGACATTGGTGGAACGAGTATCAAATATGCTAAATT
TAATCAAGAAGGTAAGCGGG

Product: phosphotransferase system mannitol-specific enzyme IIA

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MLLDRVTLEDIEVNLKAKDWREAIQTSARLLLERGAIKESYIDGMIQSVENNGPYIVIAKHIALAHTRPEFGVIRGGLTF
ATLADGVAFGSDMFDPVKLVITLAATDSESHLEVLSELAEVLMDEEKVTRLIEANSSQAFYDELTKEG

Sequences:

>Translated_148_residues
MLLDRVTLEDIEVNLKAKDWREAIQTSARLLLERGAIKESYIDGMIQSVENNGPYIVIAKHIALAHTRPEFGVIRGGLTF
ATLADGVAFGSDMFDPVKLVITLAATDSESHLEVLSELAEVLMDEEKVTRLIEANSSQAFYDELTKEG
>Mature_148_residues
MLLDRVTLEDIEVNLKAKDWREAIQTSARLLLERGAIKESYIDGMIQSVENNGPYIVIAKHIALAHTRPEFGVIRGGLTF
ATLADGVAFGSDMFDPVKLVITLAATDSESHLEVLSELAEVLMDEEKVTRLIEANSSQAFYDELTKEG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790755, Length=131, Percent_Identity=33.587786259542, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1789302, Length=112, Percent_Identity=41.9642857142857, Blast_Score=90, Evalue=5e-20,
Organism=Escherichia coli, GI2367359, Length=133, Percent_Identity=34.5864661654135, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16351; Mature: 16351

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDRVTLEDIEVNLKAKDWREAIQTSARLLLERGAIKESYIDGMIQSVENNGPYIVIAK
CCCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
HIALAHTRPEFGVIRGGLTFATLADGVAFGSDMFDPVKLVITLAATDSESHLEVLSELAE
EEHHHCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHEEEEECCCCHHHHHHHHHHHH
VLMDEEKVTRLIEANSSQAFYDELTKEG
HHHCHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MLLDRVTLEDIEVNLKAKDWREAIQTSARLLLERGAIKESYIDGMIQSVENNGPYIVIAK
CCCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
HIALAHTRPEFGVIRGGLTFATLADGVAFGSDMFDPVKLVITLAATDSESHLEVLSELAE
EEHHHCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHEEEEECCCCHHHHHHHHHHHH
VLMDEEKVTRLIEANSSQAFYDELTKEG
HHHCHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]