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The map label for this gene is cocE [H]

Identifier: 226225307

GI number: 226225307

Start: 2804587

End: 2806254

Strand: Reverse

Name: cocE [H]

Synonym: Lm4b_02730

Alternate gene names: 226225307

Gene position: 2806254-2804587 (Counterclockwise)

Preceding gene: 226225308

Following gene: 226225306

Centisome position: 96.35

GC content: 39.81

Gene sequence:

>1668_bases
GTGAAACATAATCAATTAATTATTGAAACGGATGTCCCAGCTGAAATGCGCGATGGTGTAACGCTTTACGCAGATATATA
TCGCCCAGCAGACGCGGGGGAATATCCAGTTTTACTTACAAGGTTACCTTATAGTAAATCATACGGGCTTCACTTTATTC
GTCCTAATATTTTGGCGGAGCAAGGTTATGTTGTTATTGTTCAAGATGTTCGTGGCAGATATACGTCAGAAGGAGATTTT
GTTCCATACATAGCAGAAGTTGACGATGGATATGATACGATTGAGTGGGCTGCGAACCTGCCTTATGCTAACGGAAATGT
CGGCATGTTTGGTTTATCTTATTATGGTTATACGCAAATTTTAGCCGCAATTAGCGGGAACAAACATTTGAAAGCCATTG
CTCCAATTATGGCTCAAAATAGCATGACAGATGTTTTTAATGACCACGACGGCGCTTTAGAACTTGGAATGTGGGAAACT
TGGAATCTAGAGTCCATGCTTCCAAACATGCTAGCCCGTACTTATAAAACGCAAGATGAATTAGAAGAAGCAGTGAACAC
ATTAATGAAAAATCTAGACAATTTAGACGCACTTTATCAATTTAAACCTTATAAAGACTGGCCAGCAATCGGTCAAAATG
AAATGCCTTACTTCTCTGAGTTATTAAATTATGAACCAACACATAACCATTGGCAAAAAATCGATGCAAAAAGCAATTAT
GATAAAATTAATGTGCCAGGTCTCCATGTAGCAGGCTGGTATGACTGCTTTTTAGATAAAACAATCGCTAATTTTCAAAA
TGGTCACTTGCGAGGTCTTGGAGATAAGCTCATCATTGGTCCATGGACGCATGCTAATTTCACTCAGATGATTGGCGACC
GTGATTTTGGAATGGCGGCCACTAAATGGGGAGAGGCGAATATGCATGCTAGACATATAGAATGGTTCAATCATTGGCTG
AAAAAAGAGCCTTTACCAGCATCAGCGCCTGTTAATTATTTTGTGATGGGGTTAAATGACTGGAAAACAGCTGAAAACTG
GCCTCCTCAAAATGCAAATATGACACCATTATATTTTAAAAATGAAAAAGTGAGCGCAGAATTCACACCTCCTACAACAT
CTTCAGAAGCAAAATTTAGCTATGATCCAGAAAATCCAGTACCATCAAACGGTGGAGGGACCTTACATAAGGCACTACAT
GCAGATGGACCTCGTGACCAACAACAAATTGAACTTCGTGAAGACGTTCTTTGCTACTCAACGGAGCCACTAAAAGAAGC
AATCGAAGTTACCGGACCAATTCAAGTGAAACTCTGGGCCAAATCAGATGCACCTAACACGGATTTTACAGCGAAATTAG
TGGATGTATTTCCAGATGGAACGGCTTTTAACTTAGCAGATGGCATCATCCGGGCGGCCAAGCAACATGGCGACAATGTG
CAAAATAATATTAATGAATACACTATCGACCTTTGGGCAACAAGTAATTTATTCCAAAAAGGTCATCAAATCCGCGTTGA
AATATCTTCTAGTAATTTTCCGAGATTTGATCCAAACCCAAATACAGGCGATAACTTTATTAATTCCACTGAAAGCCAAA
TCGCCAACCAGACGATTTATCATAGTCCTGAATTTCCATCACATATTCTTCTGCCAATCGTTCGCTAG

Upstream 100 bases:

>100_bases
AAAGCGGCCCACTCATATTGAGTGGGTCGCTTTGTTTGACATAGTCTGTCGTATACGAAACAATAAAAGAGCAATCAATA
CTGAAAAGGAGATGTTTCAC

Downstream 100 bases:

>100_bases
AAATTTTGGTTTTTTGGGCGAAAAGTTTTATATACGGCACTACTAAATCATGGTAAAATATGAAAGTATCCGTCAAAAAA
ATGAAACTTATTCGAAAAGG

Product: acylase and diesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKHNQLIIETDVPAEMRDGVTLYADIYRPADAGEYPVLLTRLPYSKSYGLHFIRPNILAEQGYVVIVQDVRGRYTSEGDF
VPYIAEVDDGYDTIEWAANLPYANGNVGMFGLSYYGYTQILAAISGNKHLKAIAPIMAQNSMTDVFNDHDGALELGMWET
WNLESMLPNMLARTYKTQDELEEAVNTLMKNLDNLDALYQFKPYKDWPAIGQNEMPYFSELLNYEPTHNHWQKIDAKSNY
DKINVPGLHVAGWYDCFLDKTIANFQNGHLRGLGDKLIIGPWTHANFTQMIGDRDFGMAATKWGEANMHARHIEWFNHWL
KKEPLPASAPVNYFVMGLNDWKTAENWPPQNANMTPLYFKNEKVSAEFTPPTTSSEAKFSYDPENPVPSNGGGTLHKALH
ADGPRDQQQIELREDVLCYSTEPLKEAIEVTGPIQVKLWAKSDAPNTDFTAKLVDVFPDGTAFNLADGIIRAAKQHGDNV
QNNINEYTIDLWATSNLFQKGHQIRVEISSSNFPRFDPNPNTGDNFINSTESQIANQTIYHSPEFPSHILLPIVR

Sequences:

>Translated_555_residues
MKHNQLIIETDVPAEMRDGVTLYADIYRPADAGEYPVLLTRLPYSKSYGLHFIRPNILAEQGYVVIVQDVRGRYTSEGDF
VPYIAEVDDGYDTIEWAANLPYANGNVGMFGLSYYGYTQILAAISGNKHLKAIAPIMAQNSMTDVFNDHDGALELGMWET
WNLESMLPNMLARTYKTQDELEEAVNTLMKNLDNLDALYQFKPYKDWPAIGQNEMPYFSELLNYEPTHNHWQKIDAKSNY
DKINVPGLHVAGWYDCFLDKTIANFQNGHLRGLGDKLIIGPWTHANFTQMIGDRDFGMAATKWGEANMHARHIEWFNHWL
KKEPLPASAPVNYFVMGLNDWKTAENWPPQNANMTPLYFKNEKVSAEFTPPTTSSEAKFSYDPENPVPSNGGGTLHKALH
ADGPRDQQQIELREDVLCYSTEPLKEAIEVTGPIQVKLWAKSDAPNTDFTAKLVDVFPDGTAFNLADGIIRAAKQHGDNV
QNNINEYTIDLWATSNLFQKGHQIRVEISSSNFPRFDPNPNTGDNFINSTESQIANQTIYHSPEFPSHILLPIVR
>Mature_555_residues
MKHNQLIIETDVPAEMRDGVTLYADIYRPADAGEYPVLLTRLPYSKSYGLHFIRPNILAEQGYVVIVQDVRGRYTSEGDF
VPYIAEVDDGYDTIEWAANLPYANGNVGMFGLSYYGYTQILAAISGNKHLKAIAPIMAQNSMTDVFNDHDGALELGMWET
WNLESMLPNMLARTYKTQDELEEAVNTLMKNLDNLDALYQFKPYKDWPAIGQNEMPYFSELLNYEPTHNHWQKIDAKSNY
DKINVPGLHVAGWYDCFLDKTIANFQNGHLRGLGDKLIIGPWTHANFTQMIGDRDFGMAATKWGEANMHARHIEWFNHWL
KKEPLPASAPVNYFVMGLNDWKTAENWPPQNANMTPLYFKNEKVSAEFTPPTTSSEAKFSYDPENPVPSNGGGTLHKALH
ADGPRDQQQIELREDVLCYSTEPLKEAIEVTGPIQVKLWAKSDAPNTDFTAKLVDVFPDGTAFNLADGIIRAAKQHGDNV
QNNINEYTIDLWATSNLFQKGHQIRVEISSSNFPRFDPNPNTGDNFINSTESQIANQTIYHSPEFPSHILLPIVR

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 62819; Mature: 62819

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHNQLIIETDVPAEMRDGVTLYADIYRPADAGEYPVLLTRLPYSKSYGLHFIRPNILAE
CCCCCEEEEECCCHHHHCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEECCCEEEC
QGYVVIVQDVRGRYTSEGDFVPYIAEVDDGYDTIEWAANLPYANGNVGMFGLSYYGYTQI
CCCEEEEEECCCCCCCCCCEEEEEEECCCCCCEEHHHCCCCCCCCCEEEEEEEHHHHHHH
LAAISGNKHLKAIAPIMAQNSMTDVFNDHDGALELGMWETWNLESMLPNMLARTYKTQDE
HHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHH
LEEAVNTLMKNLDNLDALYQFKPYKDWPAIGQNEMPYFSELLNYEPTHNHWQKIDAKSNY
HHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCC
DKINVPGLHVAGWYDCFLDKTIANFQNGHLRGLGDKLIIGPWTHANFTQMIGDRDFGMAA
CEECCCCEEECHHHHHHHHHHHHHCCCCCEECCCCEEEECCCCCCCHHHHHCCCCCCCEE
TKWGEANMHARHIEWFNHWLKKEPLPASAPVNYFVMGLNDWKTAENWPPQNANMTPLYFK
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEE
NEKVSAEFTPPTTSSEAKFSYDPENPVPSNGGGTLHKALHADGPRDQQQIELREDVLCYS
CCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHEEECC
TEPLKEAIEVTGPIQVKLWAKSDAPNTDFTAKLVDVFPDGTAFNLADGIIRAAKQHGDNV
CHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHH
QNNINEYTIDLWATSNLFQKGHQIRVEISSSNFPRFDPNPNTGDNFINSTESQIANQTIY
HCCCCEEEEEEEECCHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEE
HSPEFPSHILLPIVR
CCCCCCCEEECEECC
>Mature Secondary Structure
MKHNQLIIETDVPAEMRDGVTLYADIYRPADAGEYPVLLTRLPYSKSYGLHFIRPNILAE
CCCCCEEEEECCCHHHHCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEECCCEEEC
QGYVVIVQDVRGRYTSEGDFVPYIAEVDDGYDTIEWAANLPYANGNVGMFGLSYYGYTQI
CCCEEEEEECCCCCCCCCCEEEEEEECCCCCCEEHHHCCCCCCCCCEEEEEEEHHHHHHH
LAAISGNKHLKAIAPIMAQNSMTDVFNDHDGALELGMWETWNLESMLPNMLARTYKTQDE
HHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCHHH
LEEAVNTLMKNLDNLDALYQFKPYKDWPAIGQNEMPYFSELLNYEPTHNHWQKIDAKSNY
HHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCC
DKINVPGLHVAGWYDCFLDKTIANFQNGHLRGLGDKLIIGPWTHANFTQMIGDRDFGMAA
CEECCCCEEECHHHHHHHHHHHHHCCCCCEECCCCEEEECCCCCCCHHHHHCCCCCCCEE
TKWGEANMHARHIEWFNHWLKKEPLPASAPVNYFVMGLNDWKTAENWPPQNANMTPLYFK
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEE
NEKVSAEFTPPTTSSEAKFSYDPENPVPSNGGGTLHKALHADGPRDQQQIELREDVLCYS
CCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHEEECC
TEPLKEAIEVTGPIQVKLWAKSDAPNTDFTAKLVDVFPDGTAFNLADGIIRAAKQHGDNV
CHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHH
QNNINEYTIDLWATSNLFQKGHQIRVEISSSNFPRFDPNPNTGDNFINSTESQIANQTIY
HCCCCEEEEEEEECCHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEE
HSPEFPSHILLPIVR
CCCCCCCEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]