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The map label for this gene is topB

Identifier: 226225308

GI number: 226225308

Start: 2806363

End: 2808516

Strand: Reverse

Name: topB

Synonym: Lm4b_02731

Alternate gene names: 226225308

Gene position: 2808516-2806363 (Counterclockwise)

Preceding gene: 226225309

Following gene: 226225307

Centisome position: 96.42

GC content: 40.58

Gene sequence:

>2154_bases
ATGACAAAAACATTAGTACTGGCAGAGAAACCGTCTGTCGGTAAAGATATCGGCCGGGTACTCGGGGCTAAACAAGGTAA
GAACGGCTATTTAGAAGGTGGAAAATATGTAGTAACTTGGGCGCTTGGACACCTTGTAACACTAGCTGACCCTGAGCGCT
ACGATTCGAAGTATAAAAACTGGAATATGGAAGATTTACCAATGCTTCCAGAGAAAATGAAGCTAGAACCAATCAAGCAA
ACGAGAAAGCAATATGAAACGGTTAAGAAGTTAATGAACCGTACAGATATAACCACGATCGTTATTGCAACAGATGCAGG
TAGAGAAGGAGAACTTGTAGCTCGCTGGATTATTGATTATGCGAAAATCAAGAAGCCTCTAAAAAGACTATGGATCTCCT
CTGTAACGGATAAAGCGATACGTGAAGGCTTCGAACACTTAAAACCAGGGAAAGCTTATGAAAATCTGTATCACTCTGCA
GTCGCTCGTTCTGAAGCTGACTGGGTGGTAGGAATCAATGCTACGCGTGCGTTGACAACGAAATATAACGCACAACTTTC
CTGTGGCCGAGTGCAAACGCCAACCCTTGCAATGATTCAACATCGTGAGGAAGAAATTCGTAATTTTAAACCACGTGAGT
ACTATGGAATTACCGCGCTAACCGAACAAGAAAGTTTCACGTGGAACAATGGGCAAACTTTTGATAAAGCTTTAGCAGAA
AAACTAGTAAAGTCTTTACAAGGGGAAAATGCGGTAATCACAGACGTTTCTATGAAAGAAAAGAAAAATTTCTCACCAGG
TTTATATGATTTAACCGAATTACAACGCGATGCGAATAATAGATATGATTTTTCGGCGAAAGAAACGCTGAATATTATGC
AAACATTATACGAGCGCCACAAAGTACTAACGTATCCACGAACAGATTCGCGCTTTATTTCAACAGACATTGTCCCAACA
CTAAAAGAACGTTTAGCGGCCTGCGGGGTGGGTGAAAATGCAAAAGCTGCTCGTCAAATCAGCGGAAAACCAATCAAAGC
GAACAAATCATTTGTAGATAACAGCAAAGTGAGCGACCATCACGCAATTATTCCGACCGAGCAACCTGTTTCCCTCGGAG
ATTTAAGCGATAAAGAACGGAAAATTTATGACCTTGTCGTCAAACGCTTTTTAGCAGTGCTTTCAGACCCATATATTTAT
GAAGAAACCTCTGTTAAAGCTAAAATCGGCCAAGAAGAATTCACTTTAAAAGGTAAAGTTGTCAAATCCCTTGGTTGGAA
AAGTATTTACGGAGAGTCGCGCGAACCAGATCAATTAACTAAAATGAAAAAAGGCGACACTATCCCAGTTAAACGAATCA
ATTTAGAGACGGGGAAAACAAAACCACCAGCGAGATTTAACGAGGCAACTTTACTTTCAGCTATGGAAAACCCATCTAAG
TACATGGAAACAACGAACAAGGCGCTCGCCAAAACACTGGGTGAGACAGGTGGACTTGGAACAGTTGCAACTCGTGCAGA
CATCATTGAAAAACTTTTCAATAGCTTCTCCTTAGAAAAACAAGGCAAGGACATTCATATTACTTCCAAAGGACGCCAAT
TGCTAGAATTAGTGCCCGAAGATCTGAAATCCCCAGAACTAACGGCGCGTTGGGAACAAAAATTATCTAAAATCGCAAAA
GGCGAACTAGATTATCGTAAATTCACTGAAGAAATGCGTAACTATGCGAAAAAAGCAGTAACTGAAATTAAACAAAACGA
TAAAAAATTCCGTCACGATAACATTACTTCACAAAAATGCCCAGACTGCGGTAAGCCGATGCTAAAAGTGAAAGGCAAAC
GTGGTACGATGCTAGTTTGTCAGGACCGTGAATGCGGTCATCGTGAATCTGTTTCAAGAACGACGAATGCTCGTTGCCCT
AATTGTCATAAACGAATGGAAATGCGCGGCGAAGGTGATAAACAAATTTTTGTATGTGTTTGTGGACACCGGGAGAAACT
ATCTGCCTTCCAACAACGTCGTGACAAACAGAAAAATAAAAATGTATCCAAAACAGATGTAGCTAAATTCATGAAAAAAC
AAAACAAGCAAGAAGATGAGCCGTTTAACAATCCTATGGCAGAAGCGCTTGCCAAATTAAAGCTAGATAAATAA

Upstream 100 bases:

>100_bases
AAGGCATTGGTGCGATGAAACGCGATAAATACGGTGCAGAATTCTTGGCTGTACTGCAACAAGAGGCATCGAAATAAAAT
AGAAGAGAGGGTTTTGACAG

Downstream 100 bases:

>100_bases
GAATGGACAAAGCGGCCCACTCATATTGAGTGGGTCGCTTTGTTTGACATAGTCTGTCGTATACGAAACAATAAAAGAGC
AATCAATACTGAAAAGGAGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 717; Mature: 716

Protein sequence:

>717_residues
MTKTLVLAEKPSVGKDIGRVLGAKQGKNGYLEGGKYVVTWALGHLVTLADPERYDSKYKNWNMEDLPMLPEKMKLEPIKQ
TRKQYETVKKLMNRTDITTIVIATDAGREGELVARWIIDYAKIKKPLKRLWISSVTDKAIREGFEHLKPGKAYENLYHSA
VARSEADWVVGINATRALTTKYNAQLSCGRVQTPTLAMIQHREEEIRNFKPREYYGITALTEQESFTWNNGQTFDKALAE
KLVKSLQGENAVITDVSMKEKKNFSPGLYDLTELQRDANNRYDFSAKETLNIMQTLYERHKVLTYPRTDSRFISTDIVPT
LKERLAACGVGENAKAARQISGKPIKANKSFVDNSKVSDHHAIIPTEQPVSLGDLSDKERKIYDLVVKRFLAVLSDPYIY
EETSVKAKIGQEEFTLKGKVVKSLGWKSIYGESREPDQLTKMKKGDTIPVKRINLETGKTKPPARFNEATLLSAMENPSK
YMETTNKALAKTLGETGGLGTVATRADIIEKLFNSFSLEKQGKDIHITSKGRQLLELVPEDLKSPELTARWEQKLSKIAK
GELDYRKFTEEMRNYAKKAVTEIKQNDKKFRHDNITSQKCPDCGKPMLKVKGKRGTMLVCQDRECGHRESVSRTTNARCP
NCHKRMEMRGEGDKQIFVCVCGHREKLSAFQQRRDKQKNKNVSKTDVAKFMKKQNKQEDEPFNNPMAEALAKLKLDK

Sequences:

>Translated_717_residues
MTKTLVLAEKPSVGKDIGRVLGAKQGKNGYLEGGKYVVTWALGHLVTLADPERYDSKYKNWNMEDLPMLPEKMKLEPIKQ
TRKQYETVKKLMNRTDITTIVIATDAGREGELVARWIIDYAKIKKPLKRLWISSVTDKAIREGFEHLKPGKAYENLYHSA
VARSEADWVVGINATRALTTKYNAQLSCGRVQTPTLAMIQHREEEIRNFKPREYYGITALTEQESFTWNNGQTFDKALAE
KLVKSLQGENAVITDVSMKEKKNFSPGLYDLTELQRDANNRYDFSAKETLNIMQTLYERHKVLTYPRTDSRFISTDIVPT
LKERLAACGVGENAKAARQISGKPIKANKSFVDNSKVSDHHAIIPTEQPVSLGDLSDKERKIYDLVVKRFLAVLSDPYIY
EETSVKAKIGQEEFTLKGKVVKSLGWKSIYGESREPDQLTKMKKGDTIPVKRINLETGKTKPPARFNEATLLSAMENPSK
YMETTNKALAKTLGETGGLGTVATRADIIEKLFNSFSLEKQGKDIHITSKGRQLLELVPEDLKSPELTARWEQKLSKIAK
GELDYRKFTEEMRNYAKKAVTEIKQNDKKFRHDNITSQKCPDCGKPMLKVKGKRGTMLVCQDRECGHRESVSRTTNARCP
NCHKRMEMRGEGDKQIFVCVCGHREKLSAFQQRRDKQKNKNVSKTDVAKFMKKQNKQEDEPFNNPMAEALAKLKLDK
>Mature_716_residues
TKTLVLAEKPSVGKDIGRVLGAKQGKNGYLEGGKYVVTWALGHLVTLADPERYDSKYKNWNMEDLPMLPEKMKLEPIKQT
RKQYETVKKLMNRTDITTIVIATDAGREGELVARWIIDYAKIKKPLKRLWISSVTDKAIREGFEHLKPGKAYENLYHSAV
ARSEADWVVGINATRALTTKYNAQLSCGRVQTPTLAMIQHREEEIRNFKPREYYGITALTEQESFTWNNGQTFDKALAEK
LVKSLQGENAVITDVSMKEKKNFSPGLYDLTELQRDANNRYDFSAKETLNIMQTLYERHKVLTYPRTDSRFISTDIVPTL
KERLAACGVGENAKAARQISGKPIKANKSFVDNSKVSDHHAIIPTEQPVSLGDLSDKERKIYDLVVKRFLAVLSDPYIYE
ETSVKAKIGQEEFTLKGKVVKSLGWKSIYGESREPDQLTKMKKGDTIPVKRINLETGKTKPPARFNEATLLSAMENPSKY
METTNKALAKTLGETGGLGTVATRADIIEKLFNSFSLEKQGKDIHITSKGRQLLELVPEDLKSPELTARWEQKLSKIAKG
ELDYRKFTEEMRNYAKKAVTEIKQNDKKFRHDNITSQKCPDCGKPMLKVKGKRGTMLVCQDRECGHRESVSRTTNARCPN
CHKRMEMRGEGDKQIFVCVCGHREKLSAFQQRRDKQKNKNVSKTDVAKFMKKQNKQEDEPFNNPMAEALAKLKLDK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=617, Percent_Identity=24.4732576985413, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI10835218, Length=684, Percent_Identity=23.8304093567251, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=32.8502415458937, Blast_Score=341, Evalue=1e-94,
Organism=Escherichia coli, GI1787529, Length=688, Percent_Identity=25.5813953488372, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI32563869, Length=636, Percent_Identity=26.5723270440252, Blast_Score=166, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17555378, Length=555, Percent_Identity=24.8648648648649, Blast_Score=117, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=654, Percent_Identity=24.4648318042813, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24585251, Length=623, Percent_Identity=26.645264847512, Blast_Score=156, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24640096, Length=621, Percent_Identity=23.8325281803543, Blast_Score=123, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81550; Mature: 81418

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLVLAEKPSVGKDIGRVLGAKQGKNGYLEGGKYVVTWALGHLVTLADPERYDSKYKN
CCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHEEECCCCHHHHHHHCC
WNMEDLPMLPEKMKLEPIKQTRKQYETVKKLMNRTDITTIVIATDAGREGELVARWIIDY
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHH
AKIKKPLKRLWISSVTDKAIREGFEHLKPGKAYENLYHSAVARSEADWVVGINATRALTT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHEEEE
KYNAQLSCGRVQTPTLAMIQHREEEIRNFKPREYYGITALTEQESFTWNNGQTFDKALAE
ECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECEEEEECCCCEECCCCCHHHHHHHH
KLVKSLQGENAVITDVSMKEKKNFSPGLYDLTELQRDANNRYDFSAKETLNIMQTLYERH
HHHHHHCCCCEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KVLTYPRTDSRFISTDIVPTLKERLAACGVGENAKAARQISGKPIKANKSFVDNSKVSDH
HEEECCCCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
HAIIPTEQPVSLGDLSDKERKIYDLVVKRFLAVLSDPYIYEETSVKAKIGQEEFTLKGKV
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHCCCHHHHHHHHH
VKSLGWKSIYGESREPDQLTKMKKGDTIPVKRINLETGKTKPPARFNEATLLSAMENPSK
HHHCCCHHHCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHH
YMETTNKALAKTLGETGGLGTVATRADIIEKLFNSFSLEKQGKDIHITSKGRQLLELVPE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHH
DLKSPELTARWEQKLSKIAKGELDYRKFTEEMRNYAKKAVTEIKQNDKKFRHDNITSQKC
HHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDCGKPMLKVKGKRGTMLVCQDRECGHRESVSRTTNARCPNCHKRMEMRGEGDKQIFVCV
CCCCCCCEEECCCCCCEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEE
CGHREKLSAFQQRRDKQKNKNVSKTDVAKFMKKQNKQEDEPFNNPMAEALAKLKLDK
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TKTLVLAEKPSVGKDIGRVLGAKQGKNGYLEGGKYVVTWALGHLVTLADPERYDSKYKN
CCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHEEECCCCHHHHHHHCC
WNMEDLPMLPEKMKLEPIKQTRKQYETVKKLMNRTDITTIVIATDAGREGELVARWIIDY
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHH
AKIKKPLKRLWISSVTDKAIREGFEHLKPGKAYENLYHSAVARSEADWVVGINATRALTT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHEEEE
KYNAQLSCGRVQTPTLAMIQHREEEIRNFKPREYYGITALTEQESFTWNNGQTFDKALAE
ECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCEECEEEEECCCCEECCCCCHHHHHHHH
KLVKSLQGENAVITDVSMKEKKNFSPGLYDLTELQRDANNRYDFSAKETLNIMQTLYERH
HHHHHHCCCCEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KVLTYPRTDSRFISTDIVPTLKERLAACGVGENAKAARQISGKPIKANKSFVDNSKVSDH
HEEECCCCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
HAIIPTEQPVSLGDLSDKERKIYDLVVKRFLAVLSDPYIYEETSVKAKIGQEEFTLKGKV
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHCCCHHHHHHHHH
VKSLGWKSIYGESREPDQLTKMKKGDTIPVKRINLETGKTKPPARFNEATLLSAMENPSK
HHHCCCHHHCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHH
YMETTNKALAKTLGETGGLGTVATRADIIEKLFNSFSLEKQGKDIHITSKGRQLLELVPE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHH
DLKSPELTARWEQKLSKIAKGELDYRKFTEEMRNYAKKAVTEIKQNDKKFRHDNITSQKC
HHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDCGKPMLKVKGKRGTMLVCQDRECGHRESVSRTTNARCPNCHKRMEMRGEGDKQIFVCV
CCCCCCCEEECCCCCCEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEE
CGHREKLSAFQQRRDKQKNKNVSKTDVAKFMKKQNKQEDEPFNNPMAEALAKLKLDK
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA