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The map label for this gene is dacA [H]

Identifier: 226225306

GI number: 226225306

Start: 2803071

End: 2804408

Strand: Reverse

Name: dacA [H]

Synonym: Lm4b_02729

Alternate gene names: 226225306

Gene position: 2804408-2803071 (Counterclockwise)

Preceding gene: 226225307

Following gene: 226225299

Centisome position: 96.28

GC content: 39.39

Gene sequence:

>1338_bases
GTGAAAAACATAATTAAAAAAACAGGGATTGTAATCATGGCAGCATCCCTTGCAGTAAGTGGCTTCTTAGTAAGCCCGAA
AGCAGCACAAGCCGCAGAAGCACCAAATGTAAATGCAAACGCAGCAATCGCAATTGAAGAAAGTACTGGTAAAATTTTAT
ATTCAAAAGACGCTGACAAGCTAATGGGCATCGCATCCATGACAAAAATGATGGACGAATACTTACTACTGGAAGCAATT
GACAAAGGACAAATTAAATGGGACGACAAAGTAACCATTTCTGAATATGCTTACAAAGTTTCACAAGACACATCACTATC
GAACGTACCACTTAGACTTGGCGAAGAATACACTGTACAAGAATTATATGAAGCAATGGCGATTTACTCTGCAAACGGAG
CAGCAATTGCTATTTCCGAAAAAATTGCAGGTTCTGAAAAAGAATTTGTCGATGCTATGAATAAAAAAGCGGACGAACTT
AATCTAGGCGAGCACCAATTCGTTAACGCTACGGGCCTTAATAACGAAGACTTAAAAGGCGGTCAGCAAGTAGGTGGACC
GAAAGACGAAAACAAAATGACAGCTCGCGGCATGGCGAAGTTAGCAAAACACTTAATAAATGATTACCCAGATGTACTTA
AAACCGCTAGTACAACTAAGAAAGAGTTCCGAAAAGGTACATCAGACCAAATTGATATGACAAACTGGAACTGGTTATTA
CCGGGCTTGATTTACGGGCGCAAAGGTGTAGACGGTTTGAAAACAGGAACAACTGATTATGCTGGAATGTGCTTGACTGC
AACCGCTGTACAAGATGGAATGCGAGTTATCACAGTCGTACTCCATGCAAATGGTGGAGCGCCTGGCGAGCATACAAGTG
CTCGTTTTGATGAAACAAACAAAATGCTCGATTATGCTTTTAATAATTTCAAAGTAAAAGAAGTGCAAAAAGCAGGATCA
AAAGTAAAAAATCCTTCTACAATTGCAGTAGATAAAGGAAAAGAAGATACAGTCGGACTAGTTACAAAAGATGCAGTGAA
GCTAGTTGTACCGAAGAATGGCAATGAACCTAAGTTAAATACAAAGGTAACAATGAAAGAAAAAACAATAGAAGCACCAG
TTAAAAAAGGTACGGAAGTTGGTAAAATGGAAGTATCTCTAAAAGATGGCGACAAGTTAGGCTATGTTGACGGAAAACAA
ACAGAAACAATTGACGTACTAACTGCTAGCGACGTTGAAAAAGCGAACTGGTTCATGCTCTCCGCACAAGCGGTAGGTTC
TTTCTTTACAGGCATTGGTAATTACGTTTCTGATGGTGTAAAAGGGTGGTTTAATTAA

Upstream 100 bases:

>100_bases
AAATGAAACTTATTCGAAAAGGGAATTTTTATGATAGGTTTCTTTATTAAAATATAGATAAAATGAGTTAATAATTTACT
CAAATGAAGGAGAAGTTGCA

Downstream 100 bases:

>100_bases
CAGTAGAACAATTGTAGTACCGATATAAATAAAAGAGTCGATTTCCTGAATTAGGAAATCGACTCTTTTATTGGCTAACT
TTAGTTCAATGGATTTCTTT

Product: D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5)

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKNIIKKTGIVIMAASLAVSGFLVSPKAAQAAEAPNVNANAAIAIEESTGKILYSKDADKLMGIASMTKMMDEYLLLEAI
DKGQIKWDDKVTISEYAYKVSQDTSLSNVPLRLGEEYTVQELYEAMAIYSANGAAIAISEKIAGSEKEFVDAMNKKADEL
NLGEHQFVNATGLNNEDLKGGQQVGGPKDENKMTARGMAKLAKHLINDYPDVLKTASTTKKEFRKGTSDQIDMTNWNWLL
PGLIYGRKGVDGLKTGTTDYAGMCLTATAVQDGMRVITVVLHANGGAPGEHTSARFDETNKMLDYAFNNFKVKEVQKAGS
KVKNPSTIAVDKGKEDTVGLVTKDAVKLVVPKNGNEPKLNTKVTMKEKTIEAPVKKGTEVGKMEVSLKDGDKLGYVDGKQ
TETIDVLTASDVEKANWFMLSAQAVGSFFTGIGNYVSDGVKGWFN

Sequences:

>Translated_445_residues
MKNIIKKTGIVIMAASLAVSGFLVSPKAAQAAEAPNVNANAAIAIEESTGKILYSKDADKLMGIASMTKMMDEYLLLEAI
DKGQIKWDDKVTISEYAYKVSQDTSLSNVPLRLGEEYTVQELYEAMAIYSANGAAIAISEKIAGSEKEFVDAMNKKADEL
NLGEHQFVNATGLNNEDLKGGQQVGGPKDENKMTARGMAKLAKHLINDYPDVLKTASTTKKEFRKGTSDQIDMTNWNWLL
PGLIYGRKGVDGLKTGTTDYAGMCLTATAVQDGMRVITVVLHANGGAPGEHTSARFDETNKMLDYAFNNFKVKEVQKAGS
KVKNPSTIAVDKGKEDTVGLVTKDAVKLVVPKNGNEPKLNTKVTMKEKTIEAPVKKGTEVGKMEVSLKDGDKLGYVDGKQ
TETIDVLTASDVEKANWFMLSAQAVGSFFTGIGNYVSDGVKGWFN
>Mature_445_residues
MKNIIKKTGIVIMAASLAVSGFLVSPKAAQAAEAPNVNANAAIAIEESTGKILYSKDADKLMGIASMTKMMDEYLLLEAI
DKGQIKWDDKVTISEYAYKVSQDTSLSNVPLRLGEEYTVQELYEAMAIYSANGAAIAISEKIAGSEKEFVDAMNKKADEL
NLGEHQFVNATGLNNEDLKGGQQVGGPKDENKMTARGMAKLAKHLINDYPDVLKTASTTKKEFRKGTSDQIDMTNWNWLL
PGLIYGRKGVDGLKTGTTDYAGMCLTATAVQDGMRVITVVLHANGGAPGEHTSARFDETNKMLDYAFNNFKVKEVQKAGS
KVKNPSTIAVDKGKEDTVGLVTKDAVKLVVPKNGNEPKLNTKVTMKEKTIEAPVKKGTEVGKMEVSLKDGDKLGYVDGKQ
TETIDVLTASDVEKANWFMLSAQAVGSFFTGIGNYVSDGVKGWFN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=431, Percent_Identity=31.0904872389791, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082022, Length=436, Percent_Identity=27.9816513761468, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786851, Length=416, Percent_Identity=29.3269230769231, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI87082054, Length=261, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48083; Mature: 48083

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIKKTGIVIMAASLAVSGFLVSPKAAQAAEAPNVNANAAIAIEESTGKILYSKDADK
CCCHHHHCCCEEEEEHHHHHCEEECCCHHHHCCCCCCCCCEEEEEECCCCCEEEECCHHH
LMGIASMTKMMDEYLLLEAIDKGQIKWDDKVTISEYAYKVSQDTSLSNVPLRLGEEYTVQ
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEHHHHHEECCCCCCCCCCCCCCCCCCCHH
ELYEAMAIYSANGAAIAISEKIAGSEKEFVDAMNKKADELNLGEHQFVNATGLNNEDLKG
HHHHHHHEECCCCCEEEEEHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCC
GQQVGGPKDENKMTARGMAKLAKHLINDYPDVLKTASTTKKEFRKGTSDQIDMTNWNWLL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEC
PGLIYGRKGVDGLKTGTTDYAGMCLTATAVQDGMRVITVVLHANGGAPGEHTSARFDETN
CHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHH
KMLDYAFNNFKVKEVQKAGSKVKNPSTIAVDKGKEDTVGLVTKDAVKLVVPKNGNEPKLN
HHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCEEEEECCCCCCCCCC
TKVTMKEKTIEAPVKKGTEVGKMEVSLKDGDKLGYVDGKQTETIDVLTASDVEKANWFML
EEEEEHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCEEEE
SAQAVGSFFTGIGNYVSDGVKGWFN
EHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNIIKKTGIVIMAASLAVSGFLVSPKAAQAAEAPNVNANAAIAIEESTGKILYSKDADK
CCCHHHHCCCEEEEEHHHHHCEEECCCHHHHCCCCCCCCCEEEEEECCCCCEEEECCHHH
LMGIASMTKMMDEYLLLEAIDKGQIKWDDKVTISEYAYKVSQDTSLSNVPLRLGEEYTVQ
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEHHHHHEECCCCCCCCCCCCCCCCCCCHH
ELYEAMAIYSANGAAIAISEKIAGSEKEFVDAMNKKADELNLGEHQFVNATGLNNEDLKG
HHHHHHHEECCCCCEEEEEHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCC
GQQVGGPKDENKMTARGMAKLAKHLINDYPDVLKTASTTKKEFRKGTSDQIDMTNWNWLL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEC
PGLIYGRKGVDGLKTGTTDYAGMCLTATAVQDGMRVITVVLHANGGAPGEHTSARFDETN
CHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHH
KMLDYAFNNFKVKEVQKAGSKVKNPSTIAVDKGKEDTVGLVTKDAVKLVVPKNGNEPKLN
HHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCEEEEECCCCCCCCCC
TKVTMKEKTIEAPVKKGTEVGKMEVSLKDGDKLGYVDGKQTETIDVLTASDVEKANWFML
EEEEEHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCEEEE
SAQAVGSFFTGIGNYVSDGVKGWFN
EHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]