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The map label for this gene is sepA

Identifier: 226224764

GI number: 226224764

Start: 2241124

End: 2243025

Strand: Reverse

Name: sepA

Synonym: Lm4b_02182

Alternate gene names: 226224764

Gene position: 2243025-2241124 (Counterclockwise)

Preceding gene: 226224765

Following gene: 226224762

Centisome position: 77.01

GC content: 40.01

Gene sequence:

>1902_bases
ATGACAAAAAAAGTCTTACCAAAAGAAGCGTCTGAATTTACAAAGAAAGTGAATGAGAAAGTAAAAAAAGCTTTACCATT
TGAGAACACGAAAGATTTTGAAGATGCAAAGAAAGGCTTTATTGGCACTTGGGATCATGTAAAAGTTGATACGGAAGATG
GCCATGCAGTTTGGGATTTGGATGATTATAAGTTTATCGAAGGAGAAGCACCTGATTCTGTTAACCCAAGTTTGTGGCGT
ATCGCTCAACTTAATATGACAAATGGTTTATTCAAAGTAACGGACCGGGTTTATCAAGTACGCGGCTTTGATATGTCGAA
TACGACGATTATGGAAGGCGATACTGGCCTTGTCATTACAGATACATTAATGTCGGTTGAAACTGCCCGTGCGGCGCTTG
AATTATACTTTGAGCATCGTCCGAAAAAACCTATTAAGGCGATTATTTATACGCATTCCCATGCCGATCATTACGGCGGT
GTAGCGGGTCTAATTAGCAAAGAAGATGTAACCTCTGGAAAAGTGGCACTTATTGGACCTGAAGGCTTTATGGAAGCGGC
GGTCAGCGAAAATATTTTTGCTGGAAATGCAATGATTCGTCGTGCGGAATTTATGTATGGTAGCCGTTTGTCACGCGGGG
AATTAGGTCAAGTGGATGCAGGACTTGGTAAAACAGCGTCCAAAGGCCACATGTCCTTACTTGCTCCAAATGATACTATT
ACGTTTGATCATGAAAAACGCGTGGTTGATGGGATTGAAGTGGAATTCTTAATGGCGCCGAATACAGAAGCTCCATCAGA
ACATATGATGTATTTCCCGCAATTTAAACTACTTAATATTGCAGAAGATGCTGTTCATAACTTGCATAACATTTTAACAT
TACGTGGTGCGCAAATTCGTGACGCTTACGAGTGGTGGAAAGATATTGACAAAGCGATTCGTGCGTTTGGTGATAAATAT
GAAGTTTGTATCGGTCAACATCACTGGCCAACGTGGGGTAATGAAGAGATTAATGACATGCTTATTCACCAACGTGACGC
TTATAAATATATGCATGACCAAACGCTTCATTTTATTAATAAAGGATTAACTGCCATTGAAGTGGCTGAAGCTGTGAAAT
TCCCTCCTGAACTAGAAGAAAAATGGTATTTACGAGGATACTACGGCACGCTTAACCATGATGTTAAAGCGATTTATCAA
TTTTATCTTGGTTGGTATAACGGAAATCCTGCTGACCTTTATCCGCTACCTCCAGAAGATGTGGCGAAAAAATACGTCGA
GTTCATGGGTGGCGTTGATGAAGTAGTGAAAAAAGCGCGTGTATCCTATGAAGCGGGTGAATATCGCTGGGTTGCGGAAG
TTGTCAAACATGCGGTTTTCGCAGACGGAGAAAACGGAGAAGCAAGAGCTCTTCTTGCAGATGCGTTAGAACAACTTGGC
TATCAATCTGAGTCCGGACCATGGCGTAATGTATTTTTAGCCGGAGCAGACGAACTGAGAAATTCTGTCCCAGATGAAGT
TATTTCTGGTGTTACACTGGATATTGTAGAAGGTATGCCGTTTAACTTGATTTTAGATTACATGGGTATTCGTTTAAATG
GGGAAAGATCCATCGGTAAACGTATCTCCATGAACTGGAAATTAACAGATGTTGATGAGAATTATCATTTACTTGTAAAT
AATTCCGTACTTATTTATCGTGAAGGTGAAGTAGACGATAAAGCTGATTTAACTTTAACTACTACTCGTGATACATTTAA
TCATATTTTCGGTGGTGTGAAAAGCTTTGAAGCAGCATTTGCTGACCAAACAGCTAAATTAGAAGGAGACGCGAAGAAAT
TTGGGGAGTTTGCTTCCTTGTTAGATGAATTTAATCCTGTCTTCAATATTGTTACACCTTAA

Upstream 100 bases:

>100_bases
CGCTCTTTTTTTGGTCCAATAATAGCAAAAGGTTTTGAATAATTTTACGGAGGTATAAAAAGAAAGTATGTGAAAATAAA
CGTGCAGGAGGAAACCGATA

Downstream 100 bases:

>100_bases
TAAAAACAAAAAACGCTTTGTTTAGCCTTTTATCAGGCTATAACAAAGCGTTTTTATTTTTCAGCTAAATATTTAATTTT
ATCTGGTACGTTTTTGATTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MTKKVLPKEASEFTKKVNEKVKKALPFENTKDFEDAKKGFIGTWDHVKVDTEDGHAVWDLDDYKFIEGEAPDSVNPSLWR
IAQLNMTNGLFKVTDRVYQVRGFDMSNTTIMEGDTGLVITDTLMSVETARAALELYFEHRPKKPIKAIIYTHSHADHYGG
VAGLISKEDVTSGKVALIGPEGFMEAAVSENIFAGNAMIRRAEFMYGSRLSRGELGQVDAGLGKTASKGHMSLLAPNDTI
TFDHEKRVVDGIEVEFLMAPNTEAPSEHMMYFPQFKLLNIAEDAVHNLHNILTLRGAQIRDAYEWWKDIDKAIRAFGDKY
EVCIGQHHWPTWGNEEINDMLIHQRDAYKYMHDQTLHFINKGLTAIEVAEAVKFPPELEEKWYLRGYYGTLNHDVKAIYQ
FYLGWYNGNPADLYPLPPEDVAKKYVEFMGGVDEVVKKARVSYEAGEYRWVAEVVKHAVFADGENGEARALLADALEQLG
YQSESGPWRNVFLAGADELRNSVPDEVISGVTLDIVEGMPFNLILDYMGIRLNGERSIGKRISMNWKLTDVDENYHLLVN
NSVLIYREGEVDDKADLTLTTTRDTFNHIFGGVKSFEAAFADQTAKLEGDAKKFGEFASLLDEFNPVFNIVTP

Sequences:

>Translated_633_residues
MTKKVLPKEASEFTKKVNEKVKKALPFENTKDFEDAKKGFIGTWDHVKVDTEDGHAVWDLDDYKFIEGEAPDSVNPSLWR
IAQLNMTNGLFKVTDRVYQVRGFDMSNTTIMEGDTGLVITDTLMSVETARAALELYFEHRPKKPIKAIIYTHSHADHYGG
VAGLISKEDVTSGKVALIGPEGFMEAAVSENIFAGNAMIRRAEFMYGSRLSRGELGQVDAGLGKTASKGHMSLLAPNDTI
TFDHEKRVVDGIEVEFLMAPNTEAPSEHMMYFPQFKLLNIAEDAVHNLHNILTLRGAQIRDAYEWWKDIDKAIRAFGDKY
EVCIGQHHWPTWGNEEINDMLIHQRDAYKYMHDQTLHFINKGLTAIEVAEAVKFPPELEEKWYLRGYYGTLNHDVKAIYQ
FYLGWYNGNPADLYPLPPEDVAKKYVEFMGGVDEVVKKARVSYEAGEYRWVAEVVKHAVFADGENGEARALLADALEQLG
YQSESGPWRNVFLAGADELRNSVPDEVISGVTLDIVEGMPFNLILDYMGIRLNGERSIGKRISMNWKLTDVDENYHLLVN
NSVLIYREGEVDDKADLTLTTTRDTFNHIFGGVKSFEAAFADQTAKLEGDAKKFGEFASLLDEFNPVFNIVTP
>Mature_632_residues
TKKVLPKEASEFTKKVNEKVKKALPFENTKDFEDAKKGFIGTWDHVKVDTEDGHAVWDLDDYKFIEGEAPDSVNPSLWRI
AQLNMTNGLFKVTDRVYQVRGFDMSNTTIMEGDTGLVITDTLMSVETARAALELYFEHRPKKPIKAIIYTHSHADHYGGV
AGLISKEDVTSGKVALIGPEGFMEAAVSENIFAGNAMIRRAEFMYGSRLSRGELGQVDAGLGKTASKGHMSLLAPNDTIT
FDHEKRVVDGIEVEFLMAPNTEAPSEHMMYFPQFKLLNIAEDAVHNLHNILTLRGAQIRDAYEWWKDIDKAIRAFGDKYE
VCIGQHHWPTWGNEEINDMLIHQRDAYKYMHDQTLHFINKGLTAIEVAEAVKFPPELEEKWYLRGYYGTLNHDVKAIYQF
YLGWYNGNPADLYPLPPEDVAKKYVEFMGGVDEVVKKARVSYEAGEYRWVAEVVKHAVFADGENGEARALLADALEQLGY
QSESGPWRNVFLAGADELRNSVPDEVISGVTLDIVEGMPFNLILDYMGIRLNGERSIGKRISMNWKLTDVDENYHLLVNN
SVLIYREGEVDDKADLTLTTTRDTFNHIFGGVKSFEAAFADQTAKLEGDAKKFGEFASLLDEFNPVFNIVTP

Specific function: Unknown

COG id: COG2015

COG function: function code Q; Alkyl sulfatase and related hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082368, Length=627, Percent_Identity=44.1786283891547, Blast_Score=565, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6324409, Length=633, Percent_Identity=47.5513428120063, Blast_Score=631, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR003033 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 71431; Mature: 71300

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVLPKEASEFTKKVNEKVKKALPFENTKDFEDAKKGFIGTWDHVKVDTEDGHAVWDL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCEEEEC
DDYKFIEGEAPDSVNPSLWRIAQLNMTNGLFKVTDRVYQVRGFDMSNTTIMEGDTGLVIT
CCCEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCCCEEEECCCCEEEE
DTLMSVETARAALELYFEHRPKKPIKAIIYTHSHADHYGGVAGLISKEDVTSGKVALIGP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEECC
EGFMEAAVSENIFAGNAMIRRAEFMYGSRLSRGELGQVDAGLGKTASKGHMSLLAPNDTI
HHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCE
TFDHEKRVVDGIEVEFLMAPNTEAPSEHMMYFPQFKLLNIAEDAVHNLHNILTLRGAQIR
EECCCHHHHCCCEEEEEECCCCCCCHHHEEECCCEEEHHHHHHHHHHHHHHHHHCCCHHH
DAYEWWKDIDKAIRAFGDKYEVCIGQHHWPTWGNEEINDMLIHQRDAYKYMHDQTLHFIN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGLTAIEVAEAVKFPPELEEKWYLRGYYGTLNHDVKAIYQFYLGWYNGNPADLYPLPPED
CCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VAKKYVEFMGGVDEVVKKARVSYEAGEYRWVAEVVKHAVFADGENGEARALLADALEQLG
HHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
YQSESGPWRNVFLAGADELRNSVPDEVISGVTLDIVEGMPFNLILDYMGIRLNGERSIGK
CCCCCCCCCEEEEECHHHHHHCCCHHHHCCCEEEHHCCCCHHHHHHHHCEEECCCCCCCC
RISMNWKLTDVDENYHLLVNNSVLIYREGEVDDKADLTLTTTRDTFNHIFGGVKSFEAAF
EEEEEEEEEECCCCEEEEEECEEEEEECCCCCCCCCEEEEECHHHHHHHHCCHHHHHHHH
ADQTAKLEGDAKKFGEFASLLDEFNPVFNIVTP
HCCHHCCCCCHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
TKKVLPKEASEFTKKVNEKVKKALPFENTKDFEDAKKGFIGTWDHVKVDTEDGHAVWDL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCEEEEC
DDYKFIEGEAPDSVNPSLWRIAQLNMTNGLFKVTDRVYQVRGFDMSNTTIMEGDTGLVIT
CCCEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCCCEEEECCCCEEEE
DTLMSVETARAALELYFEHRPKKPIKAIIYTHSHADHYGGVAGLISKEDVTSGKVALIGP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEECC
EGFMEAAVSENIFAGNAMIRRAEFMYGSRLSRGELGQVDAGLGKTASKGHMSLLAPNDTI
HHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCE
TFDHEKRVVDGIEVEFLMAPNTEAPSEHMMYFPQFKLLNIAEDAVHNLHNILTLRGAQIR
EECCCHHHHCCCEEEEEECCCCCCCHHHEEECCCEEEHHHHHHHHHHHHHHHHHCCCHHH
DAYEWWKDIDKAIRAFGDKYEVCIGQHHWPTWGNEEINDMLIHQRDAYKYMHDQTLHFIN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGLTAIEVAEAVKFPPELEEKWYLRGYYGTLNHDVKAIYQFYLGWYNGNPADLYPLPPED
CCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VAKKYVEFMGGVDEVVKKARVSYEAGEYRWVAEVVKHAVFADGENGEARALLADALEQLG
HHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
YQSESGPWRNVFLAGADELRNSVPDEVISGVTLDIVEGMPFNLILDYMGIRLNGERSIGK
CCCCCCCCCEEEEECHHHHHHCCCHHHHCCCEEEHHCCCCHHHHHHHHCEEECCCCCCCC
RISMNWKLTDVDENYHLLVNNSVLIYREGEVDDKADLTLTTTRDTFNHIFGGVKSFEAAF
EEEEEEEEEECCCCEEEEEECEEEEEECCCCCCCCCEEEEECHHHHHHHHCCHHHHHHHH
ADQTAKLEGDAKKFGEFASLLDEFNPVFNIVTP
HCCHHCCCCCHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]