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The map label for this gene is groEL

Identifier: 226224674

GI number: 226224674

Start: 2147313

End: 2148941

Strand: Reverse

Name: groEL

Synonym: Lm4b_02089

Alternate gene names: 226224674

Gene position: 2148941-2147313 (Counterclockwise)

Preceding gene: 226224675

Following gene: 226224673

Centisome position: 73.78

GC content: 41.8

Gene sequence:

>1629_bases
ATGGCAAAAGATATTAAATTTAGTGAAGATGCTCGTCGTGCCATGTTACGTGGTGTCGACCAATTAGCAAACGCAGTAAA
AGTAACACTTGGCCCAAAAGGTCGTAATGTTGTTTTAGAAAAAAAATTCGGTTCTCCGTTAATTACAAATGATGGGGTAA
CGATTGCAAAAGAAATCGAATTAGAAGACCCATTTGAAAATATGGGAGCAAAACTTGTATCTGAAGTTGCTTCTAAAACC
AATGATGTTGCTGGGGACGGAACTACAACAGCTACCGTTTTAGCGCAAGCAATGATTCAAGAAGGCTTGAAAAACGTAAC
AGCTGGAGCAAATCCAGTAGGCGTTCGCCGCGGTATCGAAAAAGCCGTAGCAACAGCTATCGAAGAATTAAAAGCTATCT
CTAAACCAATTGAAAGCAAAGAGTCTATCGCTCAAGTTGCTGCTATTTCTTCTGGTGATGAAGAAGTTGGTAAATTAATC
GCAGAAGCAATGGAACGTGTTGGTAACGACGGCGTTATCACTATTGAAGAATCCAAAGGCTTTGCAACAGAATTAGATGT
AGTAGAAGGTATGCAATTTGACCGTGGCTACACTAGCCCATACATGGTAACTGATTCCGACAAAATGGAAGCAGTTCTTG
AAAAACCATATATTTTAATTACAGACAAAAAAATCAACAACATCCAAGAAATCTTACCAGTTTTAGAACAAGTTGTTCAA
CAAGGTCGTCCAATGTTAATCATTGCGGAAGATGTTGAAGGGGAAGCTCAAGCAACTCTTGTACTAAACAAACTTCGCGG
AACATTCAACGTAGTAGCCGTGAAAGCTCCTGGTTTTGGTGATCGTCGTAAAGCAATGCTAGAAGATATTGCTATTTTAA
CAGGCGGACAAGTCATCACAGAAGACCTAGGCTTAGAACTAAAAACAGCAACAGTTGATCAACTTGGAACAGCGAACAAA
GTAGTTGTAACAAAAGATGATACAACAATCGTAGAAGGAGCAGGCGATTCCACACAAATTAGTGCTCGCGTAAACCAAAT
CCGTGCGCAAATGGAAGAAACTACTTCTGAATTTGATAGAGAAAAATTACAAGAACGTTTAGCAAAACTTGCAGGTGGGG
TAGCTGTTGTCAAAGTCGGCGCTGCAACTGAAACAGAGCTAAAAGAACGTAAATTACGTATTGAAGATGCGCTTAACTCT
ACTCGCGCAGCTGTAGAAGAAGGTATCGTAGCTGGTGGTGGTACTGCTCTTGTAAGTATTTACAATAAAGTAGCAGCACT
AGAAGCAGAAGGTGACGTAGAAACTGGTATCAACATCGTGCTTCGTTCCCTAGAAGAACCAGTTCGCCAAATCGCGCATA
ACGCTGGACTTGAAGGTTCGGTTATCGTTGAACGCTTGAAACACGAAGCAGTTGGCGTTGGTTTCAACGCAGCTAATGGC
GAATGGGTAAACATGATTGACGCTGGTATTGTGGATCCAACAAAAGTAACACGTTCCGCACTTCAAAACGCATCATCTGT
TGCTGCACTTCTATTAACTACAGAAGCAGTCGTAGCAGACAAACCAGACGAAAACGGCCCAGCAGCTGTTCCTGATATGG
GAATGGGTGGCATGGGCGGCATGATGTAA

Upstream 100 bases:

>100_bases
AGTGACGTATGAAGGAACTGACTATCTGATTTTACGTGAAAGTGACATTTTAGCAATTACTAAATAATTAAAAAAAGAAA
TAATCGGAGGGAAATATAAA

Downstream 100 bases:

>100_bases
GGTTAACATCAGCTGACATTTTTAAAAGATCTCTGGATTTTCCAGGGATCTTTTTTATTATGATTTTATAGCAGAATATT
TTCAAGAAGTTAATAGAGGA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAKDIKFSEDARRAMLRGVDQLANAVKVTLGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDPFENMGAKLVSEVASKT
NDVAGDGTTTATVLAQAMIQEGLKNVTAGANPVGVRRGIEKAVATAIEELKAISKPIESKESIAQVAAISSGDEEVGKLI
AEAMERVGNDGVITIEESKGFATELDVVEGMQFDRGYTSPYMVTDSDKMEAVLEKPYILITDKKINNIQEILPVLEQVVQ
QGRPMLIIAEDVEGEAQATLVLNKLRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGQVITEDLGLELKTATVDQLGTANK
VVVTKDDTTIVEGAGDSTQISARVNQIRAQMEETTSEFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNS
TRAAVEEGIVAGGGTALVSIYNKVAALEAEGDVETGINIVLRSLEEPVRQIAHNAGLEGSVIVERLKHEAVGVGFNAANG
EWVNMIDAGIVDPTKVTRSALQNASSVAALLLTTEAVVADKPDENGPAAVPDMGMGGMGGMM

Sequences:

>Translated_542_residues
MAKDIKFSEDARRAMLRGVDQLANAVKVTLGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDPFENMGAKLVSEVASKT
NDVAGDGTTTATVLAQAMIQEGLKNVTAGANPVGVRRGIEKAVATAIEELKAISKPIESKESIAQVAAISSGDEEVGKLI
AEAMERVGNDGVITIEESKGFATELDVVEGMQFDRGYTSPYMVTDSDKMEAVLEKPYILITDKKINNIQEILPVLEQVVQ
QGRPMLIIAEDVEGEAQATLVLNKLRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGQVITEDLGLELKTATVDQLGTANK
VVVTKDDTTIVEGAGDSTQISARVNQIRAQMEETTSEFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNS
TRAAVEEGIVAGGGTALVSIYNKVAALEAEGDVETGINIVLRSLEEPVRQIAHNAGLEGSVIVERLKHEAVGVGFNAANG
EWVNMIDAGIVDPTKVTRSALQNASSVAALLLTTEAVVADKPDENGPAAVPDMGMGGMGGMM
>Mature_541_residues
AKDIKFSEDARRAMLRGVDQLANAVKVTLGPKGRNVVLEKKFGSPLITNDGVTIAKEIELEDPFENMGAKLVSEVASKTN
DVAGDGTTTATVLAQAMIQEGLKNVTAGANPVGVRRGIEKAVATAIEELKAISKPIESKESIAQVAAISSGDEEVGKLIA
EAMERVGNDGVITIEESKGFATELDVVEGMQFDRGYTSPYMVTDSDKMEAVLEKPYILITDKKINNIQEILPVLEQVVQQ
GRPMLIIAEDVEGEAQATLVLNKLRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGQVITEDLGLELKTATVDQLGTANKV
VVTKDDTTIVEGAGDSTQISARVNQIRAQMEETTSEFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNST
RAAVEEGIVAGGGTALVSIYNKVAALEAEGDVETGINIVLRSLEEPVRQIAHNAGLEGSVIVERLKHEAVGVGFNAANGE
WVNMIDAGIVDPTKVTRSALQNASSVAALLLTTEAVVADKPDENGPAAVPDMGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=52.1821631878558, Blast_Score=531, Evalue=1e-151,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=52.1821631878558, Blast_Score=531, Evalue=1e-151,
Organism=Homo sapiens, GI24307939, Length=373, Percent_Identity=24.6648793565684, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI5453607, Length=542, Percent_Identity=22.8782287822878, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI302058290, Length=518, Percent_Identity=22.3938223938224, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI38455427, Length=118, Percent_Identity=33.8983050847458, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI63162572, Length=549, Percent_Identity=22.9508196721311, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI58331173, Length=548, Percent_Identity=22.2627737226277, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790586, Length=523, Percent_Identity=63.4799235181644, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=531, Percent_Identity=50.2824858757062, Blast_Score=512, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=49.5283018867925, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI25144674, Length=173, Percent_Identity=31.7919075144509, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25147750, Length=561, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=53.0534351145038, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322524, Length=580, Percent_Identity=22.0689655172414, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322350, Length=571, Percent_Identity=23.292469352014, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322446, Length=529, Percent_Identity=23.2514177693762, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=51.5151515151515, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=51.5151515151515, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI45550936, Length=526, Percent_Identity=48.4790874524715, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI45550132, Length=526, Percent_Identity=48.4790874524715, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI45550935, Length=526, Percent_Identity=48.4790874524715, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI17864606, Length=551, Percent_Identity=41.923774954628, Blast_Score=442, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24584129, Length=529, Percent_Identity=36.2948960302458, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI19921262, Length=529, Percent_Identity=36.2948960302458, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI17647245, Length=170, Percent_Identity=31.1764705882353, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652903, Length=448, Percent_Identity=25, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24645179, Length=505, Percent_Identity=23.1683168316832, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583944, Length=553, Percent_Identity=22.0614828209765, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57368; Mature: 57237

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDIKFSEDARRAMLRGVDQLANAVKVTLGPKGRNVVLEKKFGSPLITNDGVTIAKEIE
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEECCCCEEEEEEC
LEDPFENMGAKLVSEVASKTNDVAGDGTTTATVLAQAMIQEGLKNVTAGANPVGVRRGIE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KAVATAIEELKAISKPIESKESIAQVAAISSGDEEVGKLIAEAMERVGNDGVITIEESKG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
FATELDVVEGMQFDRGYTSPYMVTDSDKMEAVLEKPYILITDKKINNIQEILPVLEQVVQ
CCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHH
QGRPMLIIAEDVEGEAQATLVLNKLRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGQVIT
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHH
EDLGLELKTATVDQLGTANKVVVTKDDTTIVEGAGDSTQISARVNQIRAQMEETTSEFDR
HHCCCEEEECCHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVSI
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YNKVAALEAEGDVETGINIVLRSLEEPVRQIAHNAGLEGSVIVERLKHEAVGVGFNAANG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCC
EWVNMIDAGIVDPTKVTRSALQNASSVAALLLTTEAVVADKPDENGPAAVPDMGMGGMGG
CEEEHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
MM
CC
>Mature Secondary Structure 
AKDIKFSEDARRAMLRGVDQLANAVKVTLGPKGRNVVLEKKFGSPLITNDGVTIAKEIE
CCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEECCCCEEEEEEC
LEDPFENMGAKLVSEVASKTNDVAGDGTTTATVLAQAMIQEGLKNVTAGANPVGVRRGIE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KAVATAIEELKAISKPIESKESIAQVAAISSGDEEVGKLIAEAMERVGNDGVITIEESKG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
FATELDVVEGMQFDRGYTSPYMVTDSDKMEAVLEKPYILITDKKINNIQEILPVLEQVVQ
CCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHH
QGRPMLIIAEDVEGEAQATLVLNKLRGTFNVVAVKAPGFGDRRKAMLEDIAILTGGQVIT
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHH
EDLGLELKTATVDQLGTANKVVVTKDDTTIVEGAGDSTQISARVNQIRAQMEETTSEFDR
HHCCCEEEECCHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVSI
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YNKVAALEAEGDVETGINIVLRSLEEPVRQIAHNAGLEGSVIVERLKHEAVGVGFNAANG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCC
EWVNMIDAGIVDPTKVTRSALQNASSVAALLLTTEAVVADKPDENGPAAVPDMGMGGMGG
CEEEHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
MM
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]