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The map label for this gene is manZ [H]

Identifier: 226224598

GI number: 226224598

Start: 2062024

End: 2062845

Strand: Reverse

Name: manZ [H]

Synonym: Lm4b_02012

Alternate gene names: 226224598

Gene position: 2062845-2062024 (Counterclockwise)

Preceding gene: 226224599

Following gene: 226224597

Centisome position: 70.82

GC content: 37.59

Gene sequence:

>822_bases
ATGAAGATGAAGAGTTCTGACGTATTAACAAAAAAAGACTTAATGAAAGTTTTCTGGCGCTCGTTCACGATGGAATGGTC
ATGGAACTACGAACGACAATCCAATCTCGGTTATGGATTTTCCATGCTACCAGCCTTAAACAAAATTTTTAAAAATGATA
AAGATAAACGAATTTCTTCTTATCAAAGGCATTTAGAATTTTATAACGTAACACCGTGGCTTTCCACTTTTCCGCTAGGG
ATTTCGATTGCGATGGAGGAACAATCTGCGAAAGATAAAGATTTTGACACAGACTCCATCAATAATATAAAAGTAGCGTT
AATGGGACCGCTGAGTGGGATTGGAGATTCGTTCTTCTGGGGAACTTTACGCGTTATCGCTACAGGTATTGGAACTTCCT
TAGCACTTCAAGGAAATATGTTAGGGCCGATTTTGTTCCTTCTTATTTTCAATATTCCAGCTATTCTGACTCGTTATTAT
GGATTATTTATTGGCTACAATATCGGAGCTAATTTTATCGAAAAAATTCAAAAAACTGGATTAATGGATAAACTGTCTTA
TGGTGCTTCGGTTATCGGGCTGGCCGTAGTTGGTGCAATGGTCGCCACGATGGTGAACATTAACATGCCAATGAAATTTG
GCTCTGGTGATGATGCAGTAACGATTCAAAGTGTTTTTGACGGTATCGTCCCAGGAATCCTAGCACTTGGCTTCACCTTC
TTTATTTTCTGGTTAGATAAAAAAGGACTAAAAGCACATTGGATTCTTTTATTAATCGCAGGAATTGGAATTTTAGGAGC
GTTCACAGGTTTATTAAAATAA

Upstream 100 bases:

>100_bases
TTAGCGCTAATTTTAACAGGTTATGCAACGCTTAAAAATGACAATGGTCCAAAGAATTCGAATACAGAGCCAGCACTTGC
GAATGATGGAGGTATAAATT

Downstream 100 bases:

>100_bases
GTAGGTGAAAAAATGAAAAACATGGATTATTATATCCGGTCGGAACAAAGTGTTTATGAAAATATCATCCGAAGTCGAAA
AGATCTTTTACAAAAGGTCA

Product: PTS mannose-specific enzyme IID component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKMKSSDVLTKKDLMKVFWRSFTMEWSWNYERQSNLGYGFSMLPALNKIFKNDKDKRISSYQRHLEFYNVTPWLSTFPLG
ISIAMEEQSAKDKDFDTDSINNIKVALMGPLSGIGDSFFWGTLRVIATGIGTSLALQGNMLGPILFLLIFNIPAILTRYY
GLFIGYNIGANFIEKIQKTGLMDKLSYGASVIGLAVVGAMVATMVNINMPMKFGSGDDAVTIQSVFDGIVPGILALGFTF
FIFWLDKKGLKAHWILLLIAGIGILGAFTGLLK

Sequences:

>Translated_273_residues
MKMKSSDVLTKKDLMKVFWRSFTMEWSWNYERQSNLGYGFSMLPALNKIFKNDKDKRISSYQRHLEFYNVTPWLSTFPLG
ISIAMEEQSAKDKDFDTDSINNIKVALMGPLSGIGDSFFWGTLRVIATGIGTSLALQGNMLGPILFLLIFNIPAILTRYY
GLFIGYNIGANFIEKIQKTGLMDKLSYGASVIGLAVVGAMVATMVNINMPMKFGSGDDAVTIQSVFDGIVPGILALGFTF
FIFWLDKKGLKAHWILLLIAGIGILGAFTGLLK
>Mature_273_residues
MKMKSSDVLTKKDLMKVFWRSFTMEWSWNYERQSNLGYGFSMLPALNKIFKNDKDKRISSYQRHLEFYNVTPWLSTFPLG
ISIAMEEQSAKDKDFDTDSINNIKVALMGPLSGIGDSFFWGTLRVIATGIGTSLALQGNMLGPILFLLIFNIPAILTRYY
GLFIGYNIGANFIEKIQKTGLMDKLSYGASVIGLAVVGAMVATMVNINMPMKFGSGDDAVTIQSVFDGIVPGILALGFTF
FIFWLDKKGLKAHWILLLIAGIGILGAFTGLLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=277, Percent_Identity=38.6281588447653, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1789529, Length=252, Percent_Identity=36.5079365079365, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30307; Mature: 30307

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS51108 PTS_EIID ; PS00501 SPASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKSSDVLTKKDLMKVFWRSFTMEWSWNYERQSNLGYGFSMLPALNKIFKNDKDKRISS
CCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
YQRHLEFYNVTPWLSTFPLGISIAMEEQSAKDKDFDTDSINNIKVALMGPLSGIGDSFFW
HHHHHHHHCCCCCHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHH
GTLRVIATGIGTSLALQGNMLGPILFLLIFNIPAILTRYYGLFIGYNIGANFIEKIQKTG
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LMDKLSYGASVIGLAVVGAMVATMVNINMPMKFGSGDDAVTIQSVFDGIVPGILALGFTF
CHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHHH
FIFWLDKKGLKAHWILLLIAGIGILGAFTGLLK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKMKSSDVLTKKDLMKVFWRSFTMEWSWNYERQSNLGYGFSMLPALNKIFKNDKDKRISS
CCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
YQRHLEFYNVTPWLSTFPLGISIAMEEQSAKDKDFDTDSINNIKVALMGPLSGIGDSFFW
HHHHHHHHCCCCCHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHH
GTLRVIATGIGTSLALQGNMLGPILFLLIFNIPAILTRYYGLFIGYNIGANFIEKIQKTG
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LMDKLSYGASVIGLAVVGAMVATMVNINMPMKFGSGDDAVTIQSVFDGIVPGILALGFTF
CHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHHH
FIFWLDKKGLKAHWILLLIAGIGILGAFTGLLK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]