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The map label for this gene is purR [H]

Identifier: 226224592

GI number: 226224592

Start: 2056627

End: 2057637

Strand: Reverse

Name: purR [H]

Synonym: Lm4b_02006

Alternate gene names: 226224592

Gene position: 2057637-2056627 (Counterclockwise)

Preceding gene: 226224593

Following gene: 226224591

Centisome position: 70.64

GC content: 39.66

Gene sequence:

>1011_bases
ATGGCTGCAACCATCCGCGAAATAGCAGAAAAGACAGGCGTTTCAATTACGACGATTTCTCAAATTTTGAATGGTAAAGG
GGAACGATTTAGCGACTTAACTCGTGAAAAAGTTCTGAAAACAGCAAAAGAGATGTCTTACAAACCAAACTTTTTCGCCA
AAAATATGATTGTTAGTCACACAAACACCATCGGAATGATTGTCCCGGAAGTAACGGATCCTTTTTTTTCACAAATGGTA
AAAGGGGCCGAAGATTACTTGAATAAAGAAGGTTACATGATTATGCTCTGTAACTCTTCTAATGATAAAGAACGTGAAGA
TTTATATGTAGAAGAATTATTGCACCGGGCTGTCGATGGCTTAATTATCGCCAGCCCTAATATTCTGCTTTCAGGTGTTA
TCAAACGCATGGAAGAGAAGCGTTCTCCGTATATTTTACTCGATCGTCAGCGGAATCCAAGGCAAGAAGGGAATATTGCG
ATTGATGATTTCAAAGGCGGTTATATGGCGACCGAGCATTTAATCAAACTAGGCCATACGCGCATCGGAATCATTATTTC
GGATACATCTTTTTATAACGTACATGAACGCTACGAGGGTTACTTGGCATGCATGAAAGACTATAAACTTACTGTAAAAG
ATACTTGGATTGTAAGCGGGGATCAGACGATGAATGGCGGTTATGTTGCGACGCAAAAGTTACTCCAAGAAAATCTGAGT
GCCATTTTTGCAACCAATGATTTAATGGCGATGGGAACCTACCGAGCCGCACTTGAAAAAAACCTGCATATTCCTACTGA
TCTTAGCGTCATCGGATTTGATGATATTGAACTTTCCGAATATATGACACCGCCACTTACAACCATTCGGCAACCAATCT
ATGGTATCGGCGAAATTGCTGCCGAACTATTACTGAAAAATCTCCAAAATCCAACCGAGAAATTAGAGAATCGTTTACTC
GATATTTCACTAATCAACCGTAGTAGTACAAAAAGTATTGACAACCAGTAA

Upstream 100 bases:

>100_bases
GCCAAACCAGATAATTACTAATTTTGGATGAAACCCCTTTATTATTATTCTTAAATGTGGCAAAATAAGGAGAATAGATA
ATCTGACTGGGGGAGTTTAA

Downstream 100 bases:

>100_bases
GGGAGTAAGTTATAGTGAGCATGTTAAGCACATGTTCTTGTTTAGTAAAACGGTTTACTTAAAAAGTGTAACCGTTCTCA
ATAAAGGTACATAAAATGCT

Product: transcription regulator (LacI family)

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAATIREIAEKTGVSITTISQILNGKGERFSDLTREKVLKTAKEMSYKPNFFAKNMIVSHTNTIGMIVPEVTDPFFSQMV
KGAEDYLNKEGYMIMLCNSSNDKEREDLYVEELLHRAVDGLIIASPNILLSGVIKRMEEKRSPYILLDRQRNPRQEGNIA
IDDFKGGYMATEHLIKLGHTRIGIIISDTSFYNVHERYEGYLACMKDYKLTVKDTWIVSGDQTMNGGYVATQKLLQENLS
AIFATNDLMAMGTYRAALEKNLHIPTDLSVIGFDDIELSEYMTPPLTTIRQPIYGIGEIAAELLLKNLQNPTEKLENRLL
DISLINRSSTKSIDNQ

Sequences:

>Translated_336_residues
MAATIREIAEKTGVSITTISQILNGKGERFSDLTREKVLKTAKEMSYKPNFFAKNMIVSHTNTIGMIVPEVTDPFFSQMV
KGAEDYLNKEGYMIMLCNSSNDKEREDLYVEELLHRAVDGLIIASPNILLSGVIKRMEEKRSPYILLDRQRNPRQEGNIA
IDDFKGGYMATEHLIKLGHTRIGIIISDTSFYNVHERYEGYLACMKDYKLTVKDTWIVSGDQTMNGGYVATQKLLQENLS
AIFATNDLMAMGTYRAALEKNLHIPTDLSVIGFDDIELSEYMTPPLTTIRQPIYGIGEIAAELLLKNLQNPTEKLENRLL
DISLINRSSTKSIDNQ
>Mature_335_residues
AATIREIAEKTGVSITTISQILNGKGERFSDLTREKVLKTAKEMSYKPNFFAKNMIVSHTNTIGMIVPEVTDPFFSQMVK
GAEDYLNKEGYMIMLCNSSNDKEREDLYVEELLHRAVDGLIIASPNILLSGVIKRMEEKRSPYILLDRQRNPRQEGNIAI
DDFKGGYMATEHLIKLGHTRIGIIISDTSFYNVHERYEGYLACMKDYKLTVKDTWIVSGDQTMNGGYVATQKLLQENLSA
IFATNDLMAMGTYRAALEKNLHIPTDLSVIGFDDIELSEYMTPPLTTIRQPIYGIGEIAAELLLKNLQNPTEKLENRLLD
ISLINRSSTKSIDNQ

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=35.5623100303951, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=32.2388059701493, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=33.134328358209, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=31.437125748503, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=31.7567567567568, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789068, Length=297, Percent_Identity=26.5993265993266, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=29.6875, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1787906, Length=333, Percent_Identity=25.8258258258258, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=28.486646884273, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=24.7706422018349, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790715, Length=314, Percent_Identity=25.1592356687898, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1786268, Length=311, Percent_Identity=24.7588424437299, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790192, Length=221, Percent_Identity=28.5067873303167, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789456, Length=299, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37947; Mature: 37816

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATIREIAEKTGVSITTISQILNGKGERFSDLTREKVLKTAKEMSYKPNFFAKNMIVSH
CCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCEEEEC
TNTIGMIVPEVTDPFFSQMVKGAEDYLNKEGYMIMLCNSSNDKEREDLYVEELLHRAVDG
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
LIIASPNILLSGVIKRMEEKRSPYILLDRQRNPRQEGNIAIDDFKGGYMATEHLIKLGHT
EEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RIGIIISDTSFYNVHERYEGYLACMKDYKLTVKDTWIVSGDQTMNGGYVATQKLLQENLS
EEEEEEECCCEEHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
AIFATNDLMAMGTYRAALEKNLHIPTDLSVIGFDDIELSEYMTPPLTTIRQPIYGIGEIA
HEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHH
AELLLKNLQNPTEKLENRLLDISLINRSSTKSIDNQ
HHHHHHHCCCHHHHHHHHEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AATIREIAEKTGVSITTISQILNGKGERFSDLTREKVLKTAKEMSYKPNFFAKNMIVSH
CCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCEEEEC
TNTIGMIVPEVTDPFFSQMVKGAEDYLNKEGYMIMLCNSSNDKEREDLYVEELLHRAVDG
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
LIIASPNILLSGVIKRMEEKRSPYILLDRQRNPRQEGNIAIDDFKGGYMATEHLIKLGHT
EEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RIGIIISDTSFYNVHERYEGYLACMKDYKLTVKDTWIVSGDQTMNGGYVATQKLLQENLS
EEEEEEECCCEEHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
AIFATNDLMAMGTYRAALEKNLHIPTDLSVIGFDDIELSEYMTPPLTTIRQPIYGIGEIA
HEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHH
AELLLKNLQNPTEKLENRLLDISLINRSSTKSIDNQ
HHHHHHHCCCHHHHHHHHEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA