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The map label for this gene is dfrA [H]

Identifier: 226224477

GI number: 226224477

Start: 1935276

End: 1935758

Strand: Reverse

Name: dfrA [H]

Synonym: Lm4b_01890

Alternate gene names: 226224477

Gene position: 1935758-1935276 (Counterclockwise)

Preceding gene: 226224478

Following gene: 226224476

Centisome position: 66.46

GC content: 37.47

Gene sequence:

>483_bases
ATGATTATTTTTGTTTGGGCACAAGACCGCGCTGGTAATATTGGAAAAGATAACAAAATGCCGTGGCACTTACCAGGAGA
TTTGCAGTTTTTCAAAAAAACGACGACTGGGAAAACACTTGTCATGGGGCGAAAAACCTATGAATCGTTAGGAAAAGCTT
TACCAAATAGAAAGACAATCGTATTGACACGAGATCAAGGTCTCAAGTTAGATGATGCAGAGATACTTCATTCAAAAGAA
GCTGTTTTAGCTCTTGCTGAGACAGGGGAAACAATCTATATAGTTGGTGGTGCAGAAATCTATCGTTTATTTATGGATGT
GGCAGATCAGCTGATTGTGACAAAAATTGATGCAGAGTTTGAGGCTGATACTGCGTTTCCTGAAGTGGATTGGGAGAATT
TTTCCGAAGTGGCGAAAGAATTCCATGAAAAAGATGAAAAAAATAAGTACAATTACACTTTTTATACGTATGAAAGAAAT
TAG

Upstream 100 bases:

>100_bases
AACGATTTTTGATTTTGATGTAGCGGATATTTCACTGGATGGTTATAATCCAGATCCAGCAATTAAAGCACCAATTTCAG
TATAAGGGAGGTTTTCTTGG

Downstream 100 bases:

>100_bases
TAGAAACGTTGCCTGTTTGCGGGCAACGTTTTTCGAGTGGAGTGAGTATTAATTGTTATCAAAAATGGAGATAAATAAAC
GCGCTTTGAAAGAAAATATG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MIIFVWAQDRAGNIGKDNKMPWHLPGDLQFFKKTTTGKTLVMGRKTYESLGKALPNRKTIVLTRDQGLKLDDAEILHSKE
AVLALAETGETIYIVGGAEIYRLFMDVADQLIVTKIDAEFEADTAFPEVDWENFSEVAKEFHEKDEKNKYNYTFYTYERN

Sequences:

>Translated_160_residues
MIIFVWAQDRAGNIGKDNKMPWHLPGDLQFFKKTTTGKTLVMGRKTYESLGKALPNRKTIVLTRDQGLKLDDAEILHSKE
AVLALAETGETIYIVGGAEIYRLFMDVADQLIVTKIDAEFEADTAFPEVDWENFSEVAKEFHEKDEKNKYNYTFYTYERN
>Mature_160_residues
MIIFVWAQDRAGNIGKDNKMPWHLPGDLQFFKKTTTGKTLVMGRKTYESLGKALPNRKTIVLTRDQGLKLDDAEILHSKE
AVLALAETGETIYIVGGAEIYRLFMDVADQLIVTKIDAEFEADTAFPEVDWENFSEVAKEFHEKDEKNKYNYTFYTYERN

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=173, Percent_Identity=31.2138728323699, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1786233, Length=158, Percent_Identity=39.873417721519, Blast_Score=125, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17505899, Length=179, Percent_Identity=31.2849162011173, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=136, Percent_Identity=30.1470588235294, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18422; Mature: 18422

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIFVWAQDRAGNIGKDNKMPWHLPGDLQFFKKTTTGKTLVMGRKTYESLGKALPNRKTI
CEEEEEECCCCCCCCCCCCCCEECCCCHHEEEECCCCCEEEECHHHHHHHHHHCCCCCEE
VLTRDQGLKLDDAEILHSKEAVLALAETGETIYIVGGAEIYRLFMDVADQLIVTKIDAEF
EEECCCCCCCCHHHHHHCCHHEEEEECCCCEEEEEECHHHHHHHHHHHHHHEEEEECCCC
EADTAFPEVDWENFSEVAKEFHEKDEKNKYNYTFYTYERN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
>Mature Secondary Structure
MIIFVWAQDRAGNIGKDNKMPWHLPGDLQFFKKTTTGKTLVMGRKTYESLGKALPNRKTI
CEEEEEECCCCCCCCCCCCCCEECCCCHHEEEECCCCCEEEECHHHHHHHHHHCCCCCEE
VLTRDQGLKLDDAEILHSKEAVLALAETGETIYIVGGAEIYRLFMDVADQLIVTKIDAEF
EEECCCCCCCCHHHHHHCCHHEEEEECCCCEEEEEECHHHHHHHHHHHHHHEEEEECCCC
EADTAFPEVDWENFSEVAKEFHEKDEKNKYNYTFYTYERN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]