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The map label for this gene is thyA

Identifier: 226224478

GI number: 226224478

Start: 1935774

End: 1936718

Strand: Reverse

Name: thyA

Synonym: Lm4b_01891

Alternate gene names: 226224478

Gene position: 1936718-1935774 (Counterclockwise)

Preceding gene: 226224479

Following gene: 226224477

Centisome position: 66.49

GC content: 38.2

Gene sequence:

>945_bases
ATGAAACAATATTTGGATTTAGAAAAGTACGTTTTAGAGAATGGAACACAAAAAGGAGATCGCACTGGAACTGGAACAAT
CAGCACATTTGGTTATCAAATGCGTTTTGATTTACAAGAGGGCTTTCCGATTATGACAACAAAACGGGTACCATTTAAAC
TTGTAGTAAGTGAACTGCTATGGTTTTTACATGGAGATACGAATATTCGCTACCTTTTACAGCATAATAATAATATTTGG
AATGAATGGGCTTTTGAGCGTTTTGTGAAAAGCGATGATTATAAAGGCGAAGATATGACTGACTTTGGACTGCGTGCTGA
GCGTGATTCAGCTTTCAAAGAAGTGTATCAAGCGGAGATGGAGAAGTTTAAAGCTCGGATTTTAGAAGACGAAGCGTTCG
CGAATAAATACGGTGAGCTAGGTAATATTTACGGTAAACAATGGCGCGAATGGAAGACTTCACAAGGAGAAACAATTGAT
CAGTTAGCGGATTTGATTGAAATGATTAAAACGAATCCGAACTCGCGTCGTTTGATTGTGTCTGCTTGGAATCCAAAAGA
TATTCCGAATATGGCTTTACCGCCATGTCATTCGCTATTCCAATTTTATGTAGCGGACGGAAAATTATCATGTCAGCTGT
ATCAACGTAGTGCCGATATTTTCCTTGGTGTACCGTTTAATATTGCAAGCTATGCACTTCTAACACACCTGATTGCACGT
GAAGTAGGGCTGGAAGTTGGTGAGTTTATCCATACAATGGGTGATGCGCATCTTTATAATAATCATATTGAGCAAGTGAA
AGAACAGTTGTCTAGAACACCGCACAAACTTCCAAAATTAGTGCTTTCAGATAAACCAGCAACGATTTTTGATTTTGATG
TAGCGGATATTTCACTGGATGGTTATAATCCAGATCCAGCAATTAAAGCACCAATTTCAGTATAA

Upstream 100 bases:

>100_bases
AGAAATTAGCGAACTCATCACGGCAAAAGAAACGGAGCTCGAACAAATGATGGAACGCTGGGAATTTTTATCACAATACG
CGGAATAAGGAGTGCGAACG

Downstream 100 bases:

>100_bases
GGGAGGTTTTCTTGGATGATTATTTTTGTTTGGGCACAAGACCGCGCTGGTAATATTGGAAAAGATAACAAAATGCCGTG
GCACTTACCAGGAGATTTGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKQYLDLEKYVLENGTQKGDRTGTGTISTFGYQMRFDLQEGFPIMTTKRVPFKLVVSELLWFLHGDTNIRYLLQHNNNIW
NEWAFERFVKSDDYKGEDMTDFGLRAERDSAFKEVYQAEMEKFKARILEDEAFANKYGELGNIYGKQWREWKTSQGETID
QLADLIEMIKTNPNSRRLIVSAWNPKDIPNMALPPCHSLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAR
EVGLEVGEFIHTMGDAHLYNNHIEQVKEQLSRTPHKLPKLVLSDKPATIFDFDVADISLDGYNPDPAIKAPISV

Sequences:

>Translated_314_residues
MKQYLDLEKYVLENGTQKGDRTGTGTISTFGYQMRFDLQEGFPIMTTKRVPFKLVVSELLWFLHGDTNIRYLLQHNNNIW
NEWAFERFVKSDDYKGEDMTDFGLRAERDSAFKEVYQAEMEKFKARILEDEAFANKYGELGNIYGKQWREWKTSQGETID
QLADLIEMIKTNPNSRRLIVSAWNPKDIPNMALPPCHSLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAR
EVGLEVGEFIHTMGDAHLYNNHIEQVKEQLSRTPHKLPKLVLSDKPATIFDFDVADISLDGYNPDPAIKAPISV
>Mature_314_residues
MKQYLDLEKYVLENGTQKGDRTGTGTISTFGYQMRFDLQEGFPIMTTKRVPFKLVVSELLWFLHGDTNIRYLLQHNNNIW
NEWAFERFVKSDDYKGEDMTDFGLRAERDSAFKEVYQAEMEKFKARILEDEAFANKYGELGNIYGKQWREWKTSQGETID
QLADLIEMIKTNPNSRRLIVSAWNPKDIPNMALPPCHSLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAR
EVGLEVGEFIHTMGDAHLYNNHIEQVKEQLSRTPHKLPKLVLSDKPATIFDFDVADISLDGYNPDPAIKAPISV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=320, Percent_Identity=45.625, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1789191, Length=314, Percent_Identity=50, Blast_Score=331, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI71993377, Length=321, Percent_Identity=42.6791277258567, Blast_Score=258, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6324648, Length=196, Percent_Identity=52.0408163265306, Blast_Score=197, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17137556, Length=322, Percent_Identity=45.9627329192547, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36177; Mature: 36177

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLEKYVLENGTQKGDRTGTGTISTFGYQMRFDLQEGFPIMTTKRVPFKLVVSELL
CCCHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEEECCCCCCEEEECCCHHHHHHHHHH
WFLHGDTNIRYLLQHNNNIWNEWAFERFVKSDDYKGEDMTDFGLRAERDSAFKEVYQAEM
HHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EKFKARILEDEAFANKYGELGNIYGKQWREWKTSQGETIDQLADLIEMIKTNPNSRRLIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
SAWNPKDIPNMALPPCHSLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAR
EECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHH
EVGLEVGEFIHTMGDAHLYNNHIEQVKEQLSRTPHKLPKLVLSDKPATIFDFDVADISLD
HHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECEEEEEEC
GYNPDPAIKAPISV
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLEKYVLENGTQKGDRTGTGTISTFGYQMRFDLQEGFPIMTTKRVPFKLVVSELL
CCCHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEEECCCCCCEEEECCCHHHHHHHHHH
WFLHGDTNIRYLLQHNNNIWNEWAFERFVKSDDYKGEDMTDFGLRAERDSAFKEVYQAEM
HHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EKFKARILEDEAFANKYGELGNIYGKQWREWKTSQGETIDQLADLIEMIKTNPNSRRLIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
SAWNPKDIPNMALPPCHSLFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAR
EECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHH
EVGLEVGEFIHTMGDAHLYNNHIEQVKEQLSRTPHKLPKLVLSDKPATIFDFDVADISLD
HHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECEEEEEEC
GYNPDPAIKAPISV
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]