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The map label for this gene is yxjB [H]

Identifier: 226224476

GI number: 226224476

Start: 1934348

End: 1935223

Strand: Reverse

Name: yxjB [H]

Synonym: Lm4b_01889

Alternate gene names: 226224476

Gene position: 1935223-1934348 (Counterclockwise)

Preceding gene: 226224477

Following gene: 226224475

Centisome position: 66.44

GC content: 39.95

Gene sequence:

>876_bases
TTGTTATCAAAAATGGAGATAAATAAACGCGCTTTGAAAGAAAATATGGCGCTTCTTGCTTGTCCGATTTGTGGCGAAGC
ATTCGAGTTTAAAGAGCCGCAGTCATTTGTATGCCTAGAAGGTCACGGGTTTGATATCGCCAAACCAGGTTACGTACATT
TACTAAAACAAGCGCATAAAACAAAATACGATCAAGCATTATTCGAATCCCGTAAAAAAGTGATTGCGAGTGGCTTTTTT
GAAAAATTAATCGAACGAGTGACAGAAATTATTGCGGAGAAGAAAAAAGAACAACTTATTTTATATGATGCGGGTTGCGG
GGAAGGAAGTCATTTGGCGCGAGTCGTATCAAATTTACAAGCGACTGGTGTTAATGTCCAAGCGGTTGGCTTAGATATCG
CCAAAGAGGGAGTGAAACAAGCGGCACGCGACTATCCTGGTACTTCTTGGACGGTAGCTGATTTAGCCAATTGTCCGAAT
CAAGCAGAGACGGCGGATGTCATTTTAAATATTTTATCGCCATCGAATTACGTGGAGTTCAAACGGCTTTTAAAGAAGGA
TGGGTTTTTGTTAAAAGTAGTGCCAGAAGCGAATTATTTACGCGAATTACGTGAATTTATTTATGTTGATGAGAAAAGTA
GTTATTCGAATGAGTCGGTCACATCACGATTAGCAGAAAAGTTAAGCGTAGAACATGTCGAACGAGTGACCTACAAAGCG
CCGATTGCCAAAGAATTATTTGCAGATTTTCTTGAAATGACGCCACTTGGTTGGCATATTGAAGCGGATAAAAAAAGCGA
ATTACTGGGAAACCCACCGGAAGAATTAACCGTTGACTTGCAAATTATTATCGCGAATAGAGCACATCTTTTGTAA

Upstream 100 bases:

>100_bases
GAAAAAAATAAGTACAATTACACTTTTTATACGTATGAAAGAAATTAGTAGAAACGTTGCCTGTTTGCGGGCAACGTTTT
TCGAGTGGAGTGAGTATTAA

Downstream 100 bases:

>100_bases
AAGCTATGTGAAAATTTCGCAATTTAAGCTATAATGAAGGGGAAAAATACAGAAGCTGGAGGAATGACATGAATCAATCA
GAAACGAAAGCGTTAGAAGC

Product: methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLSKMEINKRALKENMALLACPICGEAFEFKEPQSFVCLEGHGFDIAKPGYVHLLKQAHKTKYDQALFESRKKVIASGFF
EKLIERVTEIIAEKKKEQLILYDAGCGEGSHLARVVSNLQATGVNVQAVGLDIAKEGVKQAARDYPGTSWTVADLANCPN
QAETADVILNILSPSNYVEFKRLLKKDGFLLKVVPEANYLRELREFIYVDEKSSYSNESVTSRLAEKLSVEHVERVTYKA
PIAKELFADFLEMTPLGWHIEADKKSELLGNPPEELTVDLQIIIANRAHLL

Sequences:

>Translated_291_residues
MLSKMEINKRALKENMALLACPICGEAFEFKEPQSFVCLEGHGFDIAKPGYVHLLKQAHKTKYDQALFESRKKVIASGFF
EKLIERVTEIIAEKKKEQLILYDAGCGEGSHLARVVSNLQATGVNVQAVGLDIAKEGVKQAARDYPGTSWTVADLANCPN
QAETADVILNILSPSNYVEFKRLLKKDGFLLKVVPEANYLRELREFIYVDEKSSYSNESVTSRLAEKLSVEHVERVTYKA
PIAKELFADFLEMTPLGWHIEADKKSELLGNPPEELTVDLQIIIANRAHLL
>Mature_291_residues
MLSKMEINKRALKENMALLACPICGEAFEFKEPQSFVCLEGHGFDIAKPGYVHLLKQAHKTKYDQALFESRKKVIASGFF
EKLIERVTEIIAEKKKEQLILYDAGCGEGSHLARVVSNLQATGVNVQAVGLDIAKEGVKQAARDYPGTSWTVADLANCPN
QAETADVILNILSPSNYVEFKRLLKKDGFLLKVVPEANYLRELREFIYVDEKSSYSNESVTSRLAEKLSVEHVERVTYKA
PIAKELFADFLEMTPLGWHIEADKKSELLGNPPEELTVDLQIIIANRAHLL

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=276, Percent_Identity=24.2753623188406, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 32625; Mature: 32625

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKMEINKRALKENMALLACPICGEAFEFKEPQSFVCLEGHGFDIAKPGYVHLLKQAHK
CCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
TKYDQALFESRKKVIASGFFEKLIERVTEIIAEKKKEQLILYDAGCGEGSHLARVVSNLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
ATGVNVQAVGLDIAKEGVKQAARDYPGTSWTVADLANCPNQAETADVILNILSPSNYVEF
HCCCEEEEECHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
KRLLKKDGFLLKVVPEANYLRELREFIYVDEKSSYSNESVTSRLAEKLSVEHVERVTYKA
HHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PIAKELFADFLEMTPLGWHIEADKKSELLGNPPEELTVDLQIIIANRAHLL
CHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCHHHHEEEEEEEEECCCCCC
>Mature Secondary Structure
MLSKMEINKRALKENMALLACPICGEAFEFKEPQSFVCLEGHGFDIAKPGYVHLLKQAHK
CCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
TKYDQALFESRKKVIASGFFEKLIERVTEIIAEKKKEQLILYDAGCGEGSHLARVVSNLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
ATGVNVQAVGLDIAKEGVKQAARDYPGTSWTVADLANCPNQAETADVILNILSPSNYVEF
HCCCEEEEECHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
KRLLKKDGFLLKVVPEANYLRELREFIYVDEKSSYSNESVTSRLAEKLSVEHVERVTYKA
HHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PIAKELFADFLEMTPLGWHIEADKKSELLGNPPEELTVDLQIIIANRAHLL
CHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCHHHHEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]