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The map label for this gene is pyrF

Identifier: 226224435

GI number: 226224435

Start: 1893473

End: 1894174

Strand: Reverse

Name: pyrF

Synonym: Lm4b_01848

Alternate gene names: 226224435

Gene position: 1894174-1893473 (Counterclockwise)

Preceding gene: 226224436

Following gene: 226224434

Centisome position: 65.03

GC content: 41.74

Gene sequence:

>702_bases
ATGAATAAACCAATTATCGCGCTAGATTTCCAGACTTACGAAGAAGTAGAGACCTTTTTAGCCAAATTTTCGGGAGAAAC
GCTATCCGTGAAAGTCGGCATGGAACTTTTTTATAGCAACGGTCCAATCATTGTTGAAAAAATAAAGCAACAACATCATG
AAATCTTCTTGGATTTAAAACTGCATGACATCCCAAACACTGTCAAAAGTGCGATGATTGGCTTGGCAAAACTAGGCGTA
GATATGGTTAATGTACATGCGGCTGGTGGGAAGAAAATGATGGAAGCTGCCAGGGAAGGTCTGGAAATAGGTTCTTCAAG
TGGCAAACGTCCAAAAATTATTGCCGTAACTCAACTGACAAGTACAAGTGAGACAGACATGCAAACAGAACAATTAATAA
AAACAAGTCTACTTGAATCCGTCATGCATTATAGCAATTTATCGAAACAAGCTGGTTTAGACGGCGTCGTTTGCTCCGCG
CTAGAAGCAGAAGATATTAAACAACAAAATGGCGCTGATTTTTTACGTGTAACACCAGGCATCCGATTAGCAAGCGACGC
AGCCGACGATCAAATTCGTGTCGTAACACCAGAAAAAGCGCGTTTGATTGGTTCGTCAAACATTGTCGTTGGTCGTTCCA
TCACGCGCGCAAACGACCCGGTAGAAGCGTATAATCAAGTTTTAAAGGAGTGGAATGCATGA

Upstream 100 bases:

>100_bases
ACCCATTTATTTGTCCGAAATTAATTAGCGAACTACCAAAACGAATGGATGAACTCGGTATTTCTTCCCTACAAGAACTT
AAAAAGGAGCGAACAAATCA

Downstream 100 bases:

>100_bases
GCATCGAAAAACAAGTAGCTGAACAATTATTAGAAATTAAAGCTGTATTTTTAAAACCAAATGAGCCTTTCACTTGGGCA
TCTGGAATTAAATCCCCTAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MNKPIIALDFQTYEEVETFLAKFSGETLSVKVGMELFYSNGPIIVEKIKQQHHEIFLDLKLHDIPNTVKSAMIGLAKLGV
DMVNVHAAGGKKMMEAAREGLEIGSSSGKRPKIIAVTQLTSTSETDMQTEQLIKTSLLESVMHYSNLSKQAGLDGVVCSA
LEAEDIKQQNGADFLRVTPGIRLASDAADDQIRVVTPEKARLIGSSNIVVGRSITRANDPVEAYNQVLKEWNA

Sequences:

>Translated_233_residues
MNKPIIALDFQTYEEVETFLAKFSGETLSVKVGMELFYSNGPIIVEKIKQQHHEIFLDLKLHDIPNTVKSAMIGLAKLGV
DMVNVHAAGGKKMMEAAREGLEIGSSSGKRPKIIAVTQLTSTSETDMQTEQLIKTSLLESVMHYSNLSKQAGLDGVVCSA
LEAEDIKQQNGADFLRVTPGIRLASDAADDQIRVVTPEKARLIGSSNIVVGRSITRANDPVEAYNQVLKEWNA
>Mature_233_residues
MNKPIIALDFQTYEEVETFLAKFSGETLSVKVGMELFYSNGPIIVEKIKQQHHEIFLDLKLHDIPNTVKSAMIGLAKLGV
DMVNVHAAGGKKMMEAAREGLEIGSSSGKRPKIIAVTQLTSTSETDMQTEQLIKTSLLESVMHYSNLSKQAGLDGVVCSA
LEAEDIKQQNGADFLRVTPGIRLASDAADDQIRVVTPEKARLIGSSNIVVGRSITRANDPVEAYNQVLKEWNA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=238, Percent_Identity=43.2773109243698, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25456; Mature: 25456

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPIIALDFQTYEEVETFLAKFSGETLSVKVGMELFYSNGPIIVEKIKQQHHEIFLDLK
CCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCEEHHHHHHHHHEEEEEEE
LHDIPNTVKSAMIGLAKLGVDMVNVHAAGGKKMMEAAREGLEIGSSSGKRPKIIAVTQLT
ECCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEC
STSETDMQTEQLIKTSLLESVMHYSNLSKQAGLDGVVCSALEAEDIKQQNGADFLRVTPG
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
IRLASDAADDQIRVVTPEKARLIGSSNIVVGRSITRANDPVEAYNQVLKEWNA
CEEECCCCCCEEEEECCHHHHEECCCCEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNKPIIALDFQTYEEVETFLAKFSGETLSVKVGMELFYSNGPIIVEKIKQQHHEIFLDLK
CCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCEEHHHHHHHHHEEEEEEE
LHDIPNTVKSAMIGLAKLGVDMVNVHAAGGKKMMEAAREGLEIGSSSGKRPKIIAVTQLT
ECCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEC
STSETDMQTEQLIKTSLLESVMHYSNLSKQAGLDGVVCSALEAEDIKQQNGADFLRVTPG
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
IRLASDAADDQIRVVTPEKARLIGSSNIVVGRSITRANDPVEAYNQVLKEWNA
CEEECCCCCCEEEEECCHHHHEECCCCEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]