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The map label for this gene is pyrE

Identifier: 226224434

GI number: 226224434

Start: 1892847

End: 1893476

Strand: Reverse

Name: pyrE

Synonym: Lm4b_01847

Alternate gene names: 226224434

Gene position: 1893476-1892847 (Counterclockwise)

Preceding gene: 226224435

Following gene: 226224431

Centisome position: 65.01

GC content: 38.73

Gene sequence:

>630_bases
ATGAGCATCGAAAAACAAGTAGCTGAACAATTATTAGAAATTAAAGCTGTATTTTTAAAACCAAATGAGCCTTTCACTTG
GGCATCTGGAATTAAATCCCCTATATATTGCGACAATCGCCTAACGCTAGGCTTTCCAAAAGTCCGTCAGTTTATCGCCA
AGTCATTAGCCGAAAAAATCAAGCAAACCTTTGGTGAAGTAGATGTTGTTGCAGGCACCGCAACTGCTGGTATTCCACAT
GCTGCATGGGTGAGTGATTTACTAGATTTACCAATGGTCTACGTCCGATCAAAAGCTAAAGAACACGGCAAAGGGAATCA
AATCGAAGGACCAATTTCTAAAGGTCAAAAAGTAGTGGTCATTGAAGATTTGATTTCAACTGGTGGAAGTTCATTAAAAG
CAGTGGAAGCGCTGGAAGAAGCTGGAGCGGAAGTAGTTGGAATCGCGGCCATATTTACATATGGACTTGCTAAAGGAAAA
CAACTGCTAGAAGAATCTGGCACAAAACTAGTAACATTAACGAACTATGATGAGCTAATTGAAGTAGCTTTAAATGAAAA
TTACGTGACTGGTGAAGATATGGAAACATTAAAAGAATGGAAGAAAAATCCAGAAAACTGGGGAAAATAA

Upstream 100 bases:

>100_bases
CGCGTTTGATTGGTTCGTCAAACATTGTCGTTGGTCGTTCCATCACGCGCGCAAACGACCCGGTAGAAGCGTATAATCAA
GTTTTAAAGGAGTGGAATGC

Downstream 100 bases:

>100_bases
AAAGAAGCTCACCTTTGTTACGGTGAGCTTCTTTTTCTATTAATATACTTTATAACTTTCACCAGTTTGTGCACCAAAGA
CGCTTTTTTCAAACGCACGA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSIEKQVAEQLLEIKAVFLKPNEPFTWASGIKSPIYCDNRLTLGFPKVRQFIAKSLAEKIKQTFGEVDVVAGTATAGIPH
AAWVSDLLDLPMVYVRSKAKEHGKGNQIEGPISKGQKVVVIEDLISTGGSSLKAVEALEEAGAEVVGIAAIFTYGLAKGK
QLLEESGTKLVTLTNYDELIEVALNENYVTGEDMETLKEWKKNPENWGK

Sequences:

>Translated_209_residues
MSIEKQVAEQLLEIKAVFLKPNEPFTWASGIKSPIYCDNRLTLGFPKVRQFIAKSLAEKIKQTFGEVDVVAGTATAGIPH
AAWVSDLLDLPMVYVRSKAKEHGKGNQIEGPISKGQKVVVIEDLISTGGSSLKAVEALEEAGAEVVGIAAIFTYGLAKGK
QLLEESGTKLVTLTNYDELIEVALNENYVTGEDMETLKEWKKNPENWGK
>Mature_208_residues
SIEKQVAEQLLEIKAVFLKPNEPFTWASGIKSPIYCDNRLTLGFPKVRQFIAKSLAEKIKQTFGEVDVVAGTATAGIPHA
AWVSDLLDLPMVYVRSKAKEHGKGNQIEGPISKGQKVVVIEDLISTGGSSLKAVEALEEAGAEVVGIAAIFTYGLAKGKQ
LLEESGTKLVTLTNYDELIEVALNENYVTGEDMETLKEWKKNPENWGK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=176, Percent_Identity=26.7045454545455, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508631, Length=168, Percent_Identity=30.3571428571429, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17554964, Length=191, Percent_Identity=28.2722513089005, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17933654, Length=138, Percent_Identity=31.1594202898551, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 22806; Mature: 22675

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00626 RCC1_2 ; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEKQVAEQLLEIKAVFLKPNEPFTWASGIKSPIYCDNRLTLGFPKVRQFIAKSLAEKI
CCHHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCEEECCCEEECCHHHHHHHHHHHHHHH
KQTFGEVDVVAGTATAGIPHAAWVSDLLDLPMVYVRSKAKEHGKGNQIEGPISKGQKVVV
HHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEE
IEDLISTGGSSLKAVEALEEAGAEVVGIAAIFTYGLAKGKQLLEESGTKLVTLTNYDELI
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
EVALNENYVTGEDMETLKEWKKNPENWGK
HHHHCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIEKQVAEQLLEIKAVFLKPNEPFTWASGIKSPIYCDNRLTLGFPKVRQFIAKSLAEKI
CHHHHHHHHHHHHHHHEECCCCCCCHHHCCCCCEEECCCEEECCHHHHHHHHHHHHHHH
KQTFGEVDVVAGTATAGIPHAAWVSDLLDLPMVYVRSKAKEHGKGNQIEGPISKGQKVVV
HHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEE
IEDLISTGGSSLKAVEALEEAGAEVVGIAAIFTYGLAKGKQLLEESGTKLVTLTNYDELI
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
EVALNENYVTGEDMETLKEWKKNPENWGK
HHHHCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]