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The map label for this gene is cbiJ [H]

Identifier: 226223800

GI number: 226223800

Start: 1205229

End: 1205981

Strand: Direct

Name: cbiJ [H]

Synonym: Lm4b_01204

Alternate gene names: 226223800

Gene position: 1205229-1205981 (Clockwise)

Preceding gene: 226223799

Following gene: 226223801

Centisome position: 41.38

GC content: 39.84

Gene sequence:

>753_bases
ATGATTTTCGTGCTAGGAGGAACTTCGGATAGCTTGGCGATTAGCGACTGGCTAACGGAAAAAAAGCAAGCCTTTATACT
CTCTGTCGCAACCGATTACGGAGAAACTTTAGCGAAACAACATGCCGAAAACGTATTTTGTGGCAGATTATCAAAAGAGG
AAATGCTCCTAAAATGGCATGCTACAAAAGTTCACCTCGTTATTGATGCGACTCATCCATTTGCGACCATTGTTTCTGAA
ACAGCGATGGAGGCATGCAAAGAAGCGGACATTCCTTATATTCGTTTTGAACGCACTAGTGAACAAACAGATAATACTTA
TTTGGTAGCAGATATTGAGGAAGCTTGTGCAGTTGCAATGAAACTTGGGAAACGAATCTTTCTTACAACTGGTAGTAAAA
ATTTACCAGAATTCGTAGCTGGTTTAAACAATCGACATATCATCGCTCGTATTTTACCAGTGTCTGATGTAATTCGTTCA
GCCGAAGAACTAGGTCTCGTTGCCGACCAGATCATTGGCATGAAAGGACCGTTCACCAAAGAAGCGAACCGAACTCAACT
AGAAATGACTGGGGCAGATGTATTAATCACTAAAGAGAGTGGAAAACAAGGTGGCTTTCAAGAAAAACTCGCAGCTGCCG
CAGAATTAAATATTCCAGTTATTGTGATTCGTCGCAAAAAATTAAATTATCCAATCGAAATAAATCATTTAAGCGAACTA
CCAGAAATTTTAACTCAATTGGAGGTCTACTAA

Upstream 100 bases:

>100_bases
TTGATTATGAGTTAGTGGATATGACAACGATGGTGATTGTAGGAAACAAAGAAACCTACGTGAAAAATGGCAAAATGATC
ACACCACGAGGTTACACACT

Downstream 100 bases:

>100_bases
TGGGAAAAGTCATGTTAATTGGTGCAGGTCCTGGAGATGCACAGCTGCTAACCGTGAAAGGTTTAGCTGCACTGCAAAAG
GCAGATGTTATTGTATACGA

Product: cobalamin biosynthesis J protein CbiJ

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIFVLGGTSDSLAISDWLTEKKQAFILSVATDYGETLAKQHAENVFCGRLSKEEMLLKWHATKVHLVIDATHPFATIVSE
TAMEACKEADIPYIRFERTSEQTDNTYLVADIEEACAVAMKLGKRIFLTTGSKNLPEFVAGLNNRHIIARILPVSDVIRS
AEELGLVADQIIGMKGPFTKEANRTQLEMTGADVLITKESGKQGGFQEKLAAAAELNIPVIVIRRKKLNYPIEINHLSEL
PEILTQLEVY

Sequences:

>Translated_250_residues
MIFVLGGTSDSLAISDWLTEKKQAFILSVATDYGETLAKQHAENVFCGRLSKEEMLLKWHATKVHLVIDATHPFATIVSE
TAMEACKEADIPYIRFERTSEQTDNTYLVADIEEACAVAMKLGKRIFLTTGSKNLPEFVAGLNNRHIIARILPVSDVIRS
AEELGLVADQIIGMKGPFTKEANRTQLEMTGADVLITKESGKQGGFQEKLAAAAELNIPVIVIRRKKLNYPIEINHLSEL
PEILTQLEVY
>Mature_250_residues
MIFVLGGTSDSLAISDWLTEKKQAFILSVATDYGETLAKQHAENVFCGRLSKEEMLLKWHATKVHLVIDATHPFATIVSE
TAMEACKEADIPYIRFERTSEQTDNTYLVADIEEACAVAMKLGKRIFLTTGSKNLPEFVAGLNNRHIIARILPVSDVIRS
AEELGLVADQIIGMKGPFTKEANRTQLEMTGADVLITKESGKQGGFQEKLAAAAELNIPVIVIRRKKLNYPIEINHLSEL
PEILTQLEVY

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y [H]

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003723 [H]

Pfam domain/function: PF02571 CbiJ [H]

EC number: 1.3.1.54

Molecular weight: Translated: 27728; Mature: 27728

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFVLGGTSDSLAISDWLTEKKQAFILSVATDYGETLAKQHAENVFCGRLSKEEMLLKWH
CEEEEECCCCCEEHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCHHHCCCCHHHEEEEEE
ATKVHLVIDATHPFATIVSETAMEACKEADIPYIRFERTSEQTDNTYLVADIEEACAVAM
CEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHH
KLGKRIFLTTGSKNLPEFVAGLNNRHIIARILPVSDVIRSAEELGLVADQIIGMKGPFTK
HHCCEEEEEECCCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
EANRTQLEMTGADVLITKESGKQGGFQEKLAAAAELNIPVIVIRRKKLNYPIEINHLSEL
CCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHH
PEILTQLEVY
HHHHHHHCCC
>Mature Secondary Structure
MIFVLGGTSDSLAISDWLTEKKQAFILSVATDYGETLAKQHAENVFCGRLSKEEMLLKWH
CEEEEECCCCCEEHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCHHHCCCCHHHEEEEEE
ATKVHLVIDATHPFATIVSETAMEACKEADIPYIRFERTSEQTDNTYLVADIEEACAVAM
CEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHH
KLGKRIFLTTGSKNLPEFVAGLNNRHIIARILPVSDVIRSAEELGLVADQIIGMKGPFTK
HHCCEEEEEECCCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
EANRTQLEMTGADVLITKESGKQGGFQEKLAAAAELNIPVIVIRRKKLNYPIEINHLSEL
CCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHH
PEILTQLEVY
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]