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The map label for this gene is hemD [H]

Identifier: 226223801

GI number: 226223801

Start: 1205981

End: 1207462

Strand: Direct

Name: hemD [H]

Synonym: Lm4b_01205

Alternate gene names: 226223801

Gene position: 1205981-1207462 (Clockwise)

Preceding gene: 226223800

Following gene: 226223802

Centisome position: 41.4

GC content: 41.77

Gene sequence:

>1482_bases
ATGGGAAAAGTCATGTTAATTGGTGCAGGTCCTGGAGATGCACAGCTGCTAACCGTGAAAGGTTTAGCTGCACTGCAAAA
GGCAGATGTTATTGTATACGATCGCCTCGTTGAACCAGCGATGCTCCAAGAACGAAAAGCTAGTTGTAAACTCATTTATG
TCGGAAAAGAACCACTTCATCATCCAATTACCCAAGAGGAAATCGAGCAAATTCTTGTTAAAGAAGCTGCGACAAATGAG
CTGGTTGTTCGTCTAAAAGCAGGAGACCCTTATGTGTTTGGTCGCGGCGGGGAAGAAGGAGAGACACTCTATAAAGCGGG
AATTCCATTTGAAGTTATTCCTGGAATCACATCCGCGATTGGTGGGCTTGCCTACGCTGGAATCCCAGTAACACATCGTG
ATTTTGCTTCGAGTTTTCACGTGATTACCGGACATTTGAAAAAAGGACGCGATCCACTAGATTGGGAAGCCCTTGCTAGA
CTGGAAGGTACGCTCGTTTTCCTGATGGGAATGACTAATTTGCCTAATATTTGTGCGAACTTACTAGAAAATGGTCGAAA
AGCCGAAACGGGTGTTGCGATTGTACAATGGGCATCTCGCGGCAAACAATTAACGGTTACCGGGAACTTACAAAACATCG
AACAAAAAGTAGCGGAATCTGGTATTTCTTCGCCGGCACTTATTGTCGTTGGTGATGTGGTGAGCTTACGACCGCAGCTT
AACTTCTTTGAGGAAATGCCACTTTTCCATACAAAAGTATTACTTCCAAGAGCACGAGCTGGAAAAAGTATGCTCGCGGA
ACAAATTCGCGATTTAGGCGGAGAAGTAACAATGTATCCCAATATTCAAGTAGTAGATGTTGTGCCAACGAAAACAAAAG
AAGAACTAAGCGAAGTAAGCGAACTTCTTTTCACATCCAAAGAAGCGGTAGAACGATTTTTTGATTATTTAGCCCAGCTT
GATTTAGATATTCGTTCTCTAAAAAATACCCATCTTACCGCTATTGGCAAACAAACAAAAGAAGCTTTTAGTGAAAAAGG
AATTATTCCAGATAGTTTCATTAAAAGGCGGAGCACGGAATTAATTTTGGAGCATTTACCACGGTTACAGAAAAATGTGA
GCAACTTAATTATCGGAAGCATAGTTGATACGGCAGAAGTTACCGCAATTTTAGCAGAAGTGGAAGCGATGGAAATGATG
CCGTTATATGATATGCGTCCTGTGACAGAACGACCATTTGACCTTGCAAGCTTTGAACAAGTTTGTTTCTCAAGTTCGCG
TTCCGTTCAAAATTTACTCGACGTACTAACAACCGAAGAAAAAGCCATTTTACAAACGAAAACAATTCTTTCCATTGGAA
GGTTCACGAGCGCAACGTTAGCATCATTCGGTATCAACAATTTTATCGAAGCCGAAAGCGCCACACCAGAGAGCTTAATT
GAACTAATCCAAAAAACAAAATTAGAAGGAGTACCACAATGA

Upstream 100 bases:

>100_bases
TTCCAGTTATTGTGATTCGTCGCAAAAAATTAAATTATCCAATCGAAATAAATCATTTAAGCGAACTACCAGAAATTTTA
ACTCAATTGGAGGTCTACTA

Downstream 100 bases:

>100_bases
AAAAAGCGATTTTAGTCGTTAGTTTTGGCACAAGTTATCCTGAAACAAGAGAAAAAACGATTGAAGCCTGTGAAAAAAAA
GTGGCCCAAGAGTTCCCAGA

Product: uroporphyrinogen-III methyltransferase/uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEPAMLQERKASCKLIYVGKEPLHHPITQEEIEQILVKEAATNE
LVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAIGGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALAR
LEGTLVFLMGMTNLPNICANLLENGRKAETGVAIVQWASRGKQLTVTGNLQNIEQKVAESGISSPALIVVGDVVSLRPQL
NFFEEMPLFHTKVLLPRARAGKSMLAEQIRDLGGEVTMYPNIQVVDVVPTKTKEELSEVSELLFTSKEAVERFFDYLAQL
DLDIRSLKNTHLTAIGKQTKEAFSEKGIIPDSFIKRRSTELILEHLPRLQKNVSNLIIGSIVDTAEVTAILAEVEAMEMM
PLYDMRPVTERPFDLASFEQVCFSSSRSVQNLLDVLTTEEKAILQTKTILSIGRFTSATLASFGINNFIEAESATPESLI
ELIQKTKLEGVPQ

Sequences:

>Translated_493_residues
MGKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEPAMLQERKASCKLIYVGKEPLHHPITQEEIEQILVKEAATNE
LVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAIGGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALAR
LEGTLVFLMGMTNLPNICANLLENGRKAETGVAIVQWASRGKQLTVTGNLQNIEQKVAESGISSPALIVVGDVVSLRPQL
NFFEEMPLFHTKVLLPRARAGKSMLAEQIRDLGGEVTMYPNIQVVDVVPTKTKEELSEVSELLFTSKEAVERFFDYLAQL
DLDIRSLKNTHLTAIGKQTKEAFSEKGIIPDSFIKRRSTELILEHLPRLQKNVSNLIIGSIVDTAEVTAILAEVEAMEMM
PLYDMRPVTERPFDLASFEQVCFSSSRSVQNLLDVLTTEEKAILQTKTILSIGRFTSATLASFGINNFIEAESATPESLI
ELIQKTKLEGVPQ
>Mature_492_residues
GKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEPAMLQERKASCKLIYVGKEPLHHPITQEEIEQILVKEAATNEL
VVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAIGGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALARL
EGTLVFLMGMTNLPNICANLLENGRKAETGVAIVQWASRGKQLTVTGNLQNIEQKVAESGISSPALIVVGDVVSLRPQLN
FFEEMPLFHTKVLLPRARAGKSMLAEQIRDLGGEVTMYPNIQVVDVVPTKTKEELSEVSELLFTSKEAVERFFDYLAQLD
LDIRSLKNTHLTAIGKQTKEAFSEKGIIPDSFIKRRSTELILEHLPRLQKNVSNLIIGSIVDTAEVTAILAEVEAMEMMP
LYDMRPVTERPFDLASFEQVCFSSSRSVQNLLDVLTTEEKAILQTKTILSIGRFTSATLASFGINNFIEAESATPESLIE
LIQKTKLEGVPQ

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=50.8264462809917, Blast_Score=238, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=35.2941176470588, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54120; Mature: 53989

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEPAMLQERKASCKLIYVGKEPLH
CCCEEEEECCCCCCEEEEEHHHHHHHHCCCHHHHHHCCHHHHHHHCCCEEEEEECCCCCC
HPITQEEIEQILVKEAATNELVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAI
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCEEEECCCCHHHHCCHHHHH
GGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALARLEGTLVFLMGMTNLPNICAN
HHHHHCCCCCCHHHHHHCHHHEEHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHH
LLENGRKAETGVAIVQWASRGKQLTVTGNLQNIEQKVAESGISSPALIVVGDVVSLRPQL
HHHCCCCCCCCHHHEEHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEECHHHCCCCC
NFFEEMPLFHTKVLLPRARAGKSMLAEQIRDLGGEVTMYPNIQVVDVVPTKTKEELSEVS
CHHHHCCHHHHHHHCCHHHCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCHHHHHHHH
ELLFTSKEAVERFFDYLAQLDLDIRSLKNTHLTAIGKQTKEAFSEKGIIPDSFIKRRSTE
HHHHCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LILEHLPRLQKNVSNLIIGSIVDTAEVTAILAEVEAMEMMPLYDMRPVTERPFDLASFEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VCFSSSRSVQNLLDVLTTEEKAILQTKTILSIGRFTSATLASFGINNFIEAESATPESLI
HHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
ELIQKTKLEGVPQ
HHHHHHCCCCCCC
>Mature Secondary Structure 
GKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEPAMLQERKASCKLIYVGKEPLH
CCEEEEECCCCCCEEEEEHHHHHHHHCCCHHHHHHCCHHHHHHHCCCEEEEEECCCCCC
HPITQEEIEQILVKEAATNELVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAI
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCEEEECCCCHHHHCCHHHHH
GGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALARLEGTLVFLMGMTNLPNICAN
HHHHHCCCCCCHHHHHHCHHHEEHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHH
LLENGRKAETGVAIVQWASRGKQLTVTGNLQNIEQKVAESGISSPALIVVGDVVSLRPQL
HHHCCCCCCCCHHHEEHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEECHHHCCCCC
NFFEEMPLFHTKVLLPRARAGKSMLAEQIRDLGGEVTMYPNIQVVDVVPTKTKEELSEVS
CHHHHCCHHHHHHHCCHHHCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCHHHHHHHH
ELLFTSKEAVERFFDYLAQLDLDIRSLKNTHLTAIGKQTKEAFSEKGIIPDSFIKRRSTE
HHHHCCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LILEHLPRLQKNVSNLIIGSIVDTAEVTAILAEVEAMEMMPLYDMRPVTERPFDLASFEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VCFSSSRSVQNLLDVLTTEEKAILQTKTILSIGRFTSATLASFGINNFIEAESATPESLI
HHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
ELIQKTKLEGVPQ
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]