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The map label for this gene is cbiH

Identifier: 226223799

GI number: 226223799

Start: 1204507

End: 1205232

Strand: Direct

Name: cbiH

Synonym: Lm4b_01203

Alternate gene names: 226223799

Gene position: 1204507-1205232 (Clockwise)

Preceding gene: 226223798

Following gene: 226223800

Centisome position: 41.35

GC content: 41.6

Gene sequence:

>726_bases
ATGATTTATGTAATCGGAATTGGCCCAGGAGATAAACGATTAATGACCGGTGAAGCACTGCAAGCAATAGAAGACGCGGA
GGTGATTGTTGGTTACGTAACGTACATCAAACTCATCAAAGAGCTAATTAAAGATAAAGAAGTAGTGAAAACAGGCATGC
GCCGCGAAATCGATCGTTGCCAAGAAGCCGTTGATATTGCACTAACAGGAAAAAAAGTAGCAGTTGTTTCAAGTGGCGAT
GCAGGAATTTACGGTATGGCTGGTCTAGTTTTAGAACTCGCAGAAAAAAGCAACCCAGATTTAGAAGTCAAAGTGATTCC
AGGAATTACCGCAAGTATTGGAGCCGCTGCTGTTCTCGGCGCGCCAATTATGCACGATTTTTGCCATATTAGTTTAAGCG
ATTTAATGACACCATGGGAAGTAATCGAAAAACGTCTAACCCACGCTGCAATGGCCGATTTTGTTGTCTGTTTTTACAAT
CCGAGAAGTAAAGGCCGCGCCAATCATTTGGCCAACGCTTTTCAAAAGATGATGGAGCATAAATCAGGGGACACAGTTGT
TGGTATTGTGAAAGATGTTGGTCGAAAAGAAGAGCGAAAAATCATTACAACGATGCGAGATATTGATTATGAGTTAGTGG
ATATGACAACGATGGTGATTGTAGGAAACAAAGAAACCTACGTGAAAAATGGCAAAATGATCACACCACGAGGTTACACA
CTATGA

Upstream 100 bases:

>100_bases
CAGACATTGGTAGTAACGGAAACGTTGTTACAAGCCGATTTGCTGAAAATGGCGTGACTTTCGCAGCAGGGAAATTAACT
AAAAATAGTGAGGTGGCAAA

Downstream 100 bases:

>100_bases
TTTTCGTGCTAGGAGGAACTTCGGATAGCTTGGCGATTAGCGACTGGCTAACGGAAAAAAAGCAAGCCTTTATACTCTCT
GTCGCAACCGATTACGGAGA

Product: precorrin methylase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIYVIGIGPGDKRLMTGEALQAIEDAEVIVGYVTYIKLIKELIKDKEVVKTGMRREIDRCQEAVDIALTGKKVAVVSSGD
AGIYGMAGLVLELAEKSNPDLEVKVIPGITASIGAAAVLGAPIMHDFCHISLSDLMTPWEVIEKRLTHAAMADFVVCFYN
PRSKGRANHLANAFQKMMEHKSGDTVVGIVKDVGRKEERKIITTMRDIDYELVDMTTMVIVGNKETYVKNGKMITPRGYT
L

Sequences:

>Translated_241_residues
MIYVIGIGPGDKRLMTGEALQAIEDAEVIVGYVTYIKLIKELIKDKEVVKTGMRREIDRCQEAVDIALTGKKVAVVSSGD
AGIYGMAGLVLELAEKSNPDLEVKVIPGITASIGAAAVLGAPIMHDFCHISLSDLMTPWEVIEKRLTHAAMADFVVCFYN
PRSKGRANHLANAFQKMMEHKSGDTVVGIVKDVGRKEERKIITTMRDIDYELVDMTTMVIVGNKETYVKNGKMITPRGYT
L
>Mature_241_residues
MIYVIGIGPGDKRLMTGEALQAIEDAEVIVGYVTYIKLIKELIKDKEVVKTGMRREIDRCQEAVDIALTGKKVAVVSSGD
AGIYGMAGLVLELAEKSNPDLEVKVIPGITASIGAAAVLGAPIMHDFCHISLSDLMTPWEVIEKRLTHAAMADFVVCFYN
PRSKGRANHLANAFQKMMEHKSGDTVVGIVKDVGRKEERKIITTMRDIDYELVDMTTMVIVGNKETYVKNGKMITPRGYT
L

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 26475; Mature: 26475

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYVIGIGPGDKRLMTGEALQAIEDAEVIVGYVTYIKLIKELIKDKEVVKTGMRREIDRC
CEEEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEAVDIALTGKKVAVVSSGDAGIYGMAGLVLELAEKSNPDLEVKVIPGITASIGAAAVLG
HHHHHEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
APIMHDFCHISLSDLMTPWEVIEKRLTHAAMADFVVCFYNPRSKGRANHLANAFQKMMEH
HHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHC
KSGDTVVGIVKDVGRKEERKIITTMRDIDYELVDMTTMVIVGNKETYVKNGKMITPRGYT
CCCCEEEEHHHHCCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCHHEEECCCEECCCCCC
L
C
>Mature Secondary Structure
MIYVIGIGPGDKRLMTGEALQAIEDAEVIVGYVTYIKLIKELIKDKEVVKTGMRREIDRC
CEEEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEAVDIALTGKKVAVVSSGDAGIYGMAGLVLELAEKSNPDLEVKVIPGITASIGAAAVLG
HHHHHEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
APIMHDFCHISLSDLMTPWEVIEKRLTHAAMADFVVCFYNPRSKGRANHLANAFQKMMEH
HHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHC
KSGDTVVGIVKDVGRKEERKIITTMRDIDYELVDMTTMVIVGNKETYVKNGKMITPRGYT
CCCCEEEEHHHHCCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCHHEEECCCEECCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]