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The map label for this gene is cobU [H]

Identifier: 226223748

GI number: 226223748

Start: 1160199

End: 1160756

Strand: Direct

Name: cobU [H]

Synonym: Lm4b_01152

Alternate gene names: 226223748

Gene position: 1160199-1160756 (Clockwise)

Preceding gene: 226223747

Following gene: 226223749

Centisome position: 39.83

GC content: 38.35

Gene sequence:

>558_bases
ATGAGTGAGATGATGCTTGTAACTGGTGGCGCTAGAAGTGGTAAAAGTAGTTTTGCTGAAAAAGAAGCTACCAAATATAA
TCGTGTTTTATATGTGGCGACCGGAATTGCATTTCAAAATGATACAGAGTTTCAAGCTAGGATAAAAAAACATCAAGCAA
CGCGTCCAGAACACTGGGATACTTTTGAAGCATTTAAAGGGATTGCTGCTTATTTGGAGCGAAATGGAAATAAATATGAT
GTAATAATGCTTGATTGCGTAACGATGTTAGTAACTAATTTGTTTTTTTCGTTGCTTGGTGAGCGCGAGTTGACAAATGA
GATTGCTGATGAAGTAGAAGCGGGAATTCAATCGGAAGTTCGCGCAATTTTAAAAGCAGGAGAGCAATCCTCAGCCAAAT
TTATTTTTGTTACGAACGAAATCGGATTAGGGGTTGTACCTGAAAACAAGTTAACACGCGTCTTTCGAGACATTATTGGT
CGTATTAATCAGCAAATTGCAACGGAAGTAGAAGAAGTCTATTTTGTCGTGAGTGGCATTCCGAAAAGGTGGAAGTAA

Upstream 100 bases:

>100_bases
CCTGCTTCTGTTATTTTAGTAGCAGTTAAAGCAAGTGAAGTAAAAGCATTTGAGCAGAACTTTCCAGCGGCTAAATGTTT
AGGAGAGTTGAGGAATTACC

Downstream 100 bases:

>100_bases
TATGAAAACATTGATTTTATTAATTCAATTCTTTACGCGAATTCCGTTACCTGTCCAAATAAATATGGATGAAATCAACT
TGAAAAAAGGGAGTGCTTTG

Product: bifunctional cobalamin biosynthesis protein CopB

Products: NA

Alternate protein names: Adenosylcobinamide kinase; Adenosylcobinamide-phosphate guanylyltransferase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSEMMLVTGGARSGKSSFAEKEATKYNRVLYVATGIAFQNDTEFQARIKKHQATRPEHWDTFEAFKGIAAYLERNGNKYD
VIMLDCVTMLVTNLFFSLLGERELTNEIADEVEAGIQSEVRAILKAGEQSSAKFIFVTNEIGLGVVPENKLTRVFRDIIG
RINQQIATEVEEVYFVVSGIPKRWK

Sequences:

>Translated_185_residues
MSEMMLVTGGARSGKSSFAEKEATKYNRVLYVATGIAFQNDTEFQARIKKHQATRPEHWDTFEAFKGIAAYLERNGNKYD
VIMLDCVTMLVTNLFFSLLGERELTNEIADEVEAGIQSEVRAILKAGEQSSAKFIFVTNEIGLGVVPENKLTRVFRDIIG
RINQQIATEVEEVYFVVSGIPKRWK
>Mature_184_residues
SEMMLVTGGARSGKSSFAEKEATKYNRVLYVATGIAFQNDTEFQARIKKHQATRPEHWDTFEAFKGIAAYLERNGNKYDV
IMLDCVTMLVTNLFFSLLGERELTNEIADEVEAGIQSEVRAILKAGEQSSAKFIFVTNEIGLGVVPENKLTRVFRDIIGR
INQQIATEVEEVYFVVSGIPKRWK

Specific function: ATP-dependent phosphorylation of adenosylcobinamide and adds GMP to adenosylcobinamide phosphate [H]

COG id: COG2087

COG function: function code H; Adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788302, Length=177, Percent_Identity=44.0677966101695, Blast_Score=161, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003203 [H]

Pfam domain/function: PF02283 CobU [H]

EC number: =2.7.1.156; =2.7.7.62 [H]

Molecular weight: Translated: 20878; Mature: 20747

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMMLVTGGARSGKSSFAEKEATKYNRVLYVATGIAFQNDTEFQARIKKHQATRPEHWD
CCCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCEECCCHHHHHHHHHHCCCCCCCCH
TFEAFKGIAAYLERNGNKYDVIMLDCVTMLVTNLFFSLLGERELTNEIADEVEAGIQSEV
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAILKAGEQSSAKFIFVTNEIGLGVVPENKLTRVFRDIIGRINQQIATEVEEVYFVVSGI
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKRWK
CCCCC
>Mature Secondary Structure 
SEMMLVTGGARSGKSSFAEKEATKYNRVLYVATGIAFQNDTEFQARIKKHQATRPEHWD
CCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCEECCCHHHHHHHHHHCCCCCCCCH
TFEAFKGIAAYLERNGNKYDVIMLDCVTMLVTNLFFSLLGERELTNEIADEVEAGIQSEV
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAILKAGEQSSAKFIFVTNEIGLGVVPENKLTRVFRDIIGRINQQIATEVEEVYFVVSGI
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKRWK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]