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The map label for this gene is cobS

Identifier: 226223749

GI number: 226223749

Start: 1160758

End: 1161504

Strand: Direct

Name: cobS

Synonym: Lm4b_01153

Alternate gene names: 226223749

Gene position: 1160758-1161504 (Clockwise)

Preceding gene: 226223748

Following gene: 226223750

Centisome position: 39.85

GC content: 40.96

Gene sequence:

>747_bases
ATGAAAACATTGATTTTATTAATTCAATTCTTTACGCGAATTCCGTTACCTGTCCAAATAAATATGGATGAAATCAACTT
GAAAAAAGGGAGTGCTTTGCTGCCGTTTGTCGGGGTGATTATTGGCGCTTGGAATTGGCTCATTTTTACTTTGGTGGCGC
TTTTGATGCCCTTACCAGTTGCGATTATTGCGGGGCTTTTTGCAGAAATTATCATTACTGGCGGTTTTCATGTTGATGCA
CTGGCGGATACGGCGGATGGACTTTTTTCTTCTCGGAAAAGAGAGCGAATGCTGGAAATTATGAAAGATAGTCGTGTAGG
CGCGAATGGCGTGATTGCGATTTGCTTTTATTTTCTTTTTTATGGCTCTTTATTCCTATCTGTTCCAGATACGCAACAAA
TTGGTTGGTTATTTTTCGTTTTACCGATTGTTGCTAAAGGCGTGACAATGTTGCTTTTTGCAAAAATGACCTATGCGGGA
TCGAAAGCAGGACTAGGTTCGATTTTTCTAGGAGTTCCTTGGTGGCCGGTTGTGATTGCGCAGGTGATCGTGTTAGTTGC
GTTAGGGCTGTTTTTCTCATATATTGGCGTTATTGCTTATGCTGGCGTGATTCTATTTACGATTATTTACCGGGCATTTG
TTTATAAACGGATTGGTGGTATGAATGGGGATACGCTTGGTGCGGGTGGACAAATGGGACAACTTATTTGTTTATTTTGT
TTAGTGCTGCTTTGGGGGTTGATTTAA

Upstream 100 bases:

>100_bases
GTCTTTCGAGACATTATTGGTCGTATTAATCAGCAAATTGCAACGGAAGTAGAAGAAGTCTATTTTGTCGTGAGTGGCAT
TCCGAAAAGGTGGAAGTAAT

Downstream 100 bases:

>100_bases
GTGCAACTTATTTTTGTGCGACACGGAGAAACGGACTGGAATGTAGCGAAAAAATATTGTGGTCAGTTAGATGTCGCGTT
AAATGAAAATGGTATTCGAC

Product: cobalamin (5'-phosphatase) synthetase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKTLILLIQFFTRIPLPVQINMDEINLKKGSALLPFVGVIIGAWNWLIFTLVALLMPLPVAIIAGLFAEIIITGGFHVDA
LADTADGLFSSRKRERMLEIMKDSRVGANGVIAICFYFLFYGSLFLSVPDTQQIGWLFFVLPIVAKGVTMLLFAKMTYAG
SKAGLGSIFLGVPWWPVVIAQVIVLVALGLFFSYIGVIAYAGVILFTIIYRAFVYKRIGGMNGDTLGAGGQMGQLICLFC
LVLLWGLI

Sequences:

>Translated_248_residues
MKTLILLIQFFTRIPLPVQINMDEINLKKGSALLPFVGVIIGAWNWLIFTLVALLMPLPVAIIAGLFAEIIITGGFHVDA
LADTADGLFSSRKRERMLEIMKDSRVGANGVIAICFYFLFYGSLFLSVPDTQQIGWLFFVLPIVAKGVTMLLFAKMTYAG
SKAGLGSIFLGVPWWPVVIAQVIVLVALGLFFSYIGVIAYAGVILFTIIYRAFVYKRIGGMNGDTLGAGGQMGQLICLFC
LVLLWGLI
>Mature_248_residues
MKTLILLIQFFTRIPLPVQINMDEINLKKGSALLPFVGVIIGAWNWLIFTLVALLMPLPVAIIAGLFAEIIITGGFHVDA
LADTADGLFSSRKRERMLEIMKDSRVGANGVIAICFYFLFYGSLFLSVPDTQQIGWLFFVLPIVAKGVTMLLFAKMTYAG
SKAGLGSIFLGVPWWPVVIAQVIVLVALGLFFSYIGVIAYAGVILFTIIYRAFVYKRIGGMNGDTLGAGGQMGQLICLFC
LVLLWGLI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=32.520325203252, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_LISMC (C1L254)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002757856.1
- GeneID:   7703292
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01153
- OMA:   ELTECAV
- ProtClustDB:   CLSK809351
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27123; Mature: 27123

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1dc30cac)-; HASH(0x1e93e0a0)-; HASH(0x1f0fd814)-; HASH(0x1e35cd54)-; HASH(0x1ed57254)-; HASH(0x1ec0bcc4)-; HASH(0x1f2000a8)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCH
>Mature Secondary Structure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HHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA