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The map label for this gene is PdhB

Identifier: 226223671

GI number: 226223671

Start: 1081134

End: 1082111

Strand: Direct

Name: PdhB

Synonym: Lm4b_01073

Alternate gene names: 226223671

Gene position: 1081134-1082111 (Clockwise)

Preceding gene: 226223670

Following gene: 226223672

Centisome position: 37.12

GC content: 41.51

Gene sequence:

>978_bases
ATGGCGCAAAAAACAATGATTCAAGCGATTACAGATGCGCTTGCAGTAGAACTTGAAAAAGACGAAAACGTATTAGTTTT
TGGGGAAGACGTTGGTAAAAACGGCGGCGTATTCCGTGCAACAGAAGGATTACAAGAAAAATTTGGCGAAGATCGTGTAT
TCGATACTCCTCTAGCAGAATCTGGTATCGGTGGTCTTGCGATTGGTCTTGCACTTGAAGGTTTCCGTCCAGTTCCAGAA
ATTCAATTCTTCGGTTTCGTATTTGAAGTAATGGACTCCGTTGCTGGTCAAATGGCTCGTATGCGTTATCGTACAGGCGG
AACTCGTACTGCTCCAATTACAATTCGCGCACCTTTTGGTGGTGGAGTTCATACACCAGAAATGCATGCGGATAACTTAG
AAGGATTAATGGCTCAATCACCTGGTTTAAAAGTGGTAATTCCATCCACTCCATATGATGCAAAAGGTCTTTTAATCTCA
GCTATTCGTGATAACGATCCAGTTATCTTCTTAGAACATATGAAATTATACCGTTCTTTCCGTGAAGAAGTTCCAGAAGG
CGAATACACAGTAGAAATTGGTAAAGCAGCTGTTCGTCGTGAAGGTACGGATGTTTCTATCATCACTTACGGTGCAATGG
TACAAGAATCAATGAAAGCAGCAGAAGCGCTTGAAAAAGATGGCGTATCTGTAGAAGTTATTGATTTACGTACAATTAGC
CCAATTGATGTGGAAACAATCATTGCTTCCGTTAAGAAAACAAACCGCGCAGTTGTAGTTCAAGAAGCGCAAAAACAAGC
GGGTATTGCAGCTAATATCGTTGCTGAAATCAACGACCATGCAATCCTTTCTCTTGAAGCTCCAGTTATGCGTGTTGCTG
CTCCAGATAGCGTATTCCCGTTCTCTCAAGCAGAAACAGTTTGGTTACCTAACCACAACGATATCATCGAACGTGTAAAA
GAAGTTATTGCATTTTAA

Upstream 100 bases:

>100_bases
AACAAACTGTAACTGATCTTCTTAAAAATATGTACGAAACACCAACTGCTCCTATCAAAGAGCAACTGGCAATCTATGAA
GCGAAGGAGTCGAAATAATC

Downstream 100 bases:

>100_bases
TTTATAATGACCGTTTTAAAACTTATGGGGAGTTTTAGAAGGGGAAAGAAATATTTTTATACTAAGGTTCAGTTTATCTG
AACCTTAGAAATTACAGGAG

Product: pyruvate dehydrogenase (E1 beta subunit)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAQKTMIQAITDALAVELEKDENVLVFGEDVGKNGGVFRATEGLQEKFGEDRVFDTPLAESGIGGLAIGLALEGFRPVPE
IQFFGFVFEVMDSVAGQMARMRYRTGGTRTAPITIRAPFGGGVHTPEMHADNLEGLMAQSPGLKVVIPSTPYDAKGLLIS
AIRDNDPVIFLEHMKLYRSFREEVPEGEYTVEIGKAAVRREGTDVSIITYGAMVQESMKAAEALEKDGVSVEVIDLRTIS
PIDVETIIASVKKTNRAVVVQEAQKQAGIAANIVAEINDHAILSLEAPVMRVAAPDSVFPFSQAETVWLPNHNDIIERVK
EVIAF

Sequences:

>Translated_325_residues
MAQKTMIQAITDALAVELEKDENVLVFGEDVGKNGGVFRATEGLQEKFGEDRVFDTPLAESGIGGLAIGLALEGFRPVPE
IQFFGFVFEVMDSVAGQMARMRYRTGGTRTAPITIRAPFGGGVHTPEMHADNLEGLMAQSPGLKVVIPSTPYDAKGLLIS
AIRDNDPVIFLEHMKLYRSFREEVPEGEYTVEIGKAAVRREGTDVSIITYGAMVQESMKAAEALEKDGVSVEVIDLRTIS
PIDVETIIASVKKTNRAVVVQEAQKQAGIAANIVAEINDHAILSLEAPVMRVAAPDSVFPFSQAETVWLPNHNDIIERVK
EVIAF
>Mature_324_residues
AQKTMIQAITDALAVELEKDENVLVFGEDVGKNGGVFRATEGLQEKFGEDRVFDTPLAESGIGGLAIGLALEGFRPVPEI
QFFGFVFEVMDSVAGQMARMRYRTGGTRTAPITIRAPFGGGVHTPEMHADNLEGLMAQSPGLKVVIPSTPYDAKGLLISA
IRDNDPVIFLEHMKLYRSFREEVPEGEYTVEIGKAAVRREGTDVSIITYGAMVQESMKAAEALEKDGVSVEVIDLRTISP
IDVETIIASVKKTNRAVVVQEAQKQAGIAANIVAEINDHAILSLEAPVMRVAAPDSVFPFSQAETVWLPNHNDIIERVKE
VIAF

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=325, Percent_Identity=41.8461538461538, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI34101272, Length=325, Percent_Identity=41.8461538461538, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=37.7300613496933, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=35.8895705521472, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=39.3188854489164, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17538422, Length=324, Percent_Identity=37.6543209876543, Blast_Score=224, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6319698, Length=331, Percent_Identity=41.0876132930514, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=39.6284829721362, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=39.6284829721362, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=37.7300613496933, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=37.7300613496933, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=38.8888888888889, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=38.8888888888889, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35298; Mature: 35167

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKTMIQAITDALAVELEKDENVLVFGEDVGKNGGVFRATEGLQEKFGEDRVFDTPLAE
CCHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCC
SGIGGLAIGLALEGFRPVPEIQFFGFVFEVMDSVAGQMARMRYRTGGTRTAPITIRAPFG
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEECCCC
GGVHTPEMHADNLEGLMAQSPGLKVVIPSTPYDAKGLLISAIRDNDPVIFLEHMKLYRSF
CCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHH
REEVPEGEYTVEIGKAAVRREGTDVSIITYGAMVQESMKAAEALEKDGVSVEVIDLRTIS
HHHCCCCCEEEEECHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
PIDVETIIASVKKTNRAVVVQEAQKQAGIAANIVAEINDHAILSLEAPVMRVAAPDSVFP
CCCHHHHHHHHHHCCCEEEEEHHHHHCCCEEEEEEECCCCEEEEECCCEEEEECCCCCCC
FSQAETVWLPNHNDIIERVKEVIAF
CCCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AQKTMIQAITDALAVELEKDENVLVFGEDVGKNGGVFRATEGLQEKFGEDRVFDTPLAE
CHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCC
SGIGGLAIGLALEGFRPVPEIQFFGFVFEVMDSVAGQMARMRYRTGGTRTAPITIRAPFG
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEECCCC
GGVHTPEMHADNLEGLMAQSPGLKVVIPSTPYDAKGLLISAIRDNDPVIFLEHMKLYRSF
CCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHH
REEVPEGEYTVEIGKAAVRREGTDVSIITYGAMVQESMKAAEALEKDGVSVEVIDLRTIS
HHHCCCCCEEEEECHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
PIDVETIIASVKKTNRAVVVQEAQKQAGIAANIVAEINDHAILSLEAPVMRVAAPDSVFP
CCCHHHHHHHHHHCCCEEEEEHHHHHCCCEEEEEEECCCCEEEEECCCEEEEECCCCCCC
FSQAETVWLPNHNDIIERVKEVIAF
CCCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629 [H]