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The map label for this gene is pdhA

Identifier: 226223670

GI number: 226223670

Start: 1080016

End: 1081131

Strand: Direct

Name: pdhA

Synonym: Lm4b_01072

Alternate gene names: 226223670

Gene position: 1080016-1081131 (Clockwise)

Preceding gene: 226223668

Following gene: 226223671

Centisome position: 37.08

GC content: 40.59

Gene sequence:

>1116_bases
ATGGCTTCTAAAACAAAGAAGGCTATTATCGACGTAAAGAAACAATTTGAGGCTGTTCATAAACAATTTGAATTAGTTCA
AATTTTGAATGAAAAAGGAGAAATCGTTAATCCAGATTTAATGCCGGATTTAACTGATGACGAGTTAGTAGAATTAATGA
CTCGCATGGTTTGGACTCGTGTACTTGACCAACGTTCTATCTCACTTAACCGTCAAGGACGTCTAGGTTTCTACGCTCCA
ACTGCTGGACAAGAAGCTTCCCAACTTGCAAGCCACTACGCACTTGAAAAACATGACTATATTCTTCCGGGTTACCGTGA
TGTGCCACAACTTATCTGGCACGGACTTCCACTTACAAAAGCGTTCTTGTTCTCTCGTGGACACTTTGTAGGTAACCAAT
TCCCTGAAGATTTAAATGTATTATCACCACAAATCATCATCGGTGCACAAATCGTGCAAGCTGCCGGTGTTGCTCTAGGA
CTTAAAAAACGTAAAAAAGATGCTGTTGTAATCACTTATACAGGTGATGGTGGTTCTTCCCAAGGTGACTTCTATGAAGG
AATGAACTTTGCAGGTGCTTACCATGCTCCAGCAATCTTCGTAGTACAAAATAACAAATTCGCGATTTCTACACCTCGTG
AAAAACAATCAGCTGCTGAAACATTAGCTCAAAAAGCAGTTGCAGCCGGAATCCCAGGCGTACAAGTAGACGGAATGGAT
CCACTTGCAGTATATGCTGTAACTAAATTCGCTCGTGAACGTGCAGTTGCTGGTGAAGGCCCAACATTAATCGAAACAAT
GACTTACCGTTATGGTCCACATACACTTTCTGGTGATGATCCAACTCGTTACCGTACAAAAGAACTTGACGGAGAATGGG
AACTTAAAGACCCAATCGTTCGCTTCCGTACTTTCTTAGAAGGTAAAGGCCTTTGGAACGAAGAAAAAGAAAATGCTGTT
ATCGATCAAGCAAAAGAAGAAATCAAAGTAGCAATTAAAGAAGCAGATGCTACACCAAAACAAACTGTAACTGATCTTCT
TAAAAATATGTACGAAACACCAACTGCTCCTATCAAAGAGCAACTGGCAATCTATGAAGCGAAGGAGTCGAAATAA

Upstream 100 bases:

>100_bases
TAGATGGGTAAATGACCGGACGAGCGTTACATAAAACGCATACCTAACTAATGAAGAATTTGAAACTGTACAATGTGAAT
GACGAAAGGGTGGATACGAA

Downstream 100 bases:

>100_bases
TCATGGCGCAAAAAACAATGATTCAAGCGATTACAGATGCGCTTGCAGTAGAACTTGAAAAAGACGAAAACGTATTAGTT
TTTGGGGAAGACGTTGGTAA

Product: pyruvate dehydrogenase (E1 alpha subunit)

Products: NA

Alternate protein names: S complex, 42 kDa subunit; Vegetative protein 220; VEG220 [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MASKTKKAIIDVKKQFEAVHKQFELVQILNEKGEIVNPDLMPDLTDDELVELMTRMVWTRVLDQRSISLNRQGRLGFYAP
TAGQEASQLASHYALEKHDYILPGYRDVPQLIWHGLPLTKAFLFSRGHFVGNQFPEDLNVLSPQIIIGAQIVQAAGVALG
LKKRKKDAVVITYTGDGGSSQGDFYEGMNFAGAYHAPAIFVVQNNKFAISTPREKQSAAETLAQKAVAAGIPGVQVDGMD
PLAVYAVTKFARERAVAGEGPTLIETMTYRYGPHTLSGDDPTRYRTKELDGEWELKDPIVRFRTFLEGKGLWNEEKENAV
IDQAKEEIKVAIKEADATPKQTVTDLLKNMYETPTAPIKEQLAIYEAKESK

Sequences:

>Translated_371_residues
MASKTKKAIIDVKKQFEAVHKQFELVQILNEKGEIVNPDLMPDLTDDELVELMTRMVWTRVLDQRSISLNRQGRLGFYAP
TAGQEASQLASHYALEKHDYILPGYRDVPQLIWHGLPLTKAFLFSRGHFVGNQFPEDLNVLSPQIIIGAQIVQAAGVALG
LKKRKKDAVVITYTGDGGSSQGDFYEGMNFAGAYHAPAIFVVQNNKFAISTPREKQSAAETLAQKAVAAGIPGVQVDGMD
PLAVYAVTKFARERAVAGEGPTLIETMTYRYGPHTLSGDDPTRYRTKELDGEWELKDPIVRFRTFLEGKGLWNEEKENAV
IDQAKEEIKVAIKEADATPKQTVTDLLKNMYETPTAPIKEQLAIYEAKESK
>Mature_370_residues
ASKTKKAIIDVKKQFEAVHKQFELVQILNEKGEIVNPDLMPDLTDDELVELMTRMVWTRVLDQRSISLNRQGRLGFYAPT
AGQEASQLASHYALEKHDYILPGYRDVPQLIWHGLPLTKAFLFSRGHFVGNQFPEDLNVLSPQIIIGAQIVQAAGVALGL
KKRKKDAVVITYTGDGGSSQGDFYEGMNFAGAYHAPAIFVVQNNKFAISTPREKQSAAETLAQKAVAAGIPGVQVDGMDP
LAVYAVTKFARERAVAGEGPTLIETMTYRYGPHTLSGDDPTRYRTKELDGEWELKDPIVRFRTFLEGKGLWNEEKENAVI
DQAKEEIKVAIKEADATPKQTVTDLLKNMYETPTAPIKEQLAIYEAKESK

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11386135, Length=347, Percent_Identity=33.4293948126801, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI258645172, Length=347, Percent_Identity=33.4293948126801, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI291084742, Length=337, Percent_Identity=29.080118694362, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI4505685, Length=337, Percent_Identity=29.080118694362, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI291084744, Length=209, Percent_Identity=34.9282296650718, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4885543, Length=334, Percent_Identity=28.1437125748503, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=27.1875, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI86563357, Length=348, Percent_Identity=31.8965517241379, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI86563355, Length=348, Percent_Identity=31.8965517241379, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI32564172, Length=335, Percent_Identity=30.1492537313433, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17536047, Length=335, Percent_Identity=30.1492537313433, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321026, Length=361, Percent_Identity=30.1939058171745, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21355903, Length=353, Percent_Identity=31.728045325779, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24639748, Length=329, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24639740, Length=324, Percent_Identity=29.9382716049383, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639744, Length=324, Percent_Identity=29.9382716049383, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28571106, Length=324, Percent_Identity=29.9382716049383, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639746, Length=291, Percent_Identity=30.5841924398625, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017596 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 41269; Mature: 41137

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKTKKAIIDVKKQFEAVHKQFELVQILNEKGEIVNPDLMPDLTDDELVELMTRMVWTR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHH
VLDQRSISLNRQGRLGFYAPTAGQEASQLASHYALEKHDYILPGYRDVPQLIWHGLPLTK
HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHCCCCHHH
AFLFSRGHFVGNQFPEDLNVLSPQIIIGAQIVQAAGVALGLKKRKKDAVVITYTGDGGSS
HHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
QGDFYEGMNFAGAYHAPAIFVVQNNKFAISTPREKQSAAETLAQKAVAAGIPGVQVDGMD
CCCHHHCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEECCCC
PLAVYAVTKFARERAVAGEGPTLIETMTYRYGPHTLSGDDPTRYRTKELDGEWELKDPIV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHH
RFRTFLEGKGLWNEEKENAVIDQAKEEIKVAIKEADATPKQTVTDLLKNMYETPTAPIKE
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHH
QLAIYEAKESK
HHHHHHHCCCC
>Mature Secondary Structure 
ASKTKKAIIDVKKQFEAVHKQFELVQILNEKGEIVNPDLMPDLTDDELVELMTRMVWTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHH
VLDQRSISLNRQGRLGFYAPTAGQEASQLASHYALEKHDYILPGYRDVPQLIWHGLPLTK
HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHCCCCHHH
AFLFSRGHFVGNQFPEDLNVLSPQIIIGAQIVQAAGVALGLKKRKKDAVVITYTGDGGSS
HHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
QGDFYEGMNFAGAYHAPAIFVVQNNKFAISTPREKQSAAETLAQKAVAAGIPGVQVDGMD
CCCHHHCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEECCCC
PLAVYAVTKFARERAVAGEGPTLIETMTYRYGPHTLSGDDPTRYRTKELDGEWELKDPIV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHH
RFRTFLEGKGLWNEEKENAVIDQAKEEIKVAIKEADATPKQTVTDLLKNMYETPTAPIKE
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHH
QLAIYEAKESK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 9298659 [H]