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The map label for this gene is pdhC

Identifier: 226223672

GI number: 226223672

Start: 1082222

End: 1083856

Strand: Direct

Name: pdhC

Synonym: Lm4b_01074

Alternate gene names: 226223672

Gene position: 1082222-1083856 (Clockwise)

Preceding gene: 226223671

Following gene: 226223673

Centisome position: 37.16

GC content: 40.24

Gene sequence:

>1635_bases
ATGGCATATTCATTTAAATTACCGGATATCGGTGAAGGTATCCATGAAGGTGAAATCGTTAAATGGTTTGTACAACCAGG
CGATAAGATTGAAGAAGATGAATCCCTATTTGAAGTACAAAACGACAAATCAGTGGAAGAAATCACTTCTCCAGTTTCAG
GAACGATTAAAGAAATCAAAGTTGCAGAAGGTACAGTTGCTACAGTTGGACAAGTACTAGTAACATTTGATGGCGTAGAA
GGTCACGAAGACGACGCCGAAGAAGAAAGCGCAGCACCAAAAGCAGAATCCACAGAATCAACTCCTGCACCCGCACAAGC
TAGCGGAAAAGGAATTTTTGAATTCAAATTACCAGATATTGGTGAAGGAATTCATGAAGGTGAAATTGTTAAATGGTTTA
TTCAACCGGGCGATAAAGTAGAAGAAGATCAGTCCATTTTTGAAGTACAAAACGACAAATCTGTCGAAGAAATTACTTCT
CCAGTAGATGGAACTGTTAAAGATATTTTAGTTAGCGAAGGAACAGTAGCAACAGTTGGTCAAGTATTAGTAACATTCGA
AGGCGATTTTGAAGGAGAAGCTAGTCATGAATCTACTCCAGAATCTCCAGCAGAAGAAGCTGAACTTACAAACAACGATG
CAACTTCCGCTCCAGTTACAGGTGGAAACGGAACGCCATCATCTAAAAAAGATCCTAATGGCCTTGTAATTGCAATGCCT
TCTGTACGTAAATATGCGCGTGAAAAAGGCGTTAATATCGCTGAAGTAGCAGGCTCTGGAAAAAATAACCGCGTAGTTAA
AGCTGATATTGATGCTTTCCTAAACGGCGAACAACCAGCTGCATCAACAACTACTGCTCAAACAGAAGAAAAAGCAGCAG
CACCAAAAGCAGAAAAAGCAGCAGCAAAACAACCAGTTGCAAGCTCCGATGCTTACCCAGAAACACGCGAAAAATTAACA
CCAACTCGTCGTGCGATTGCAAAAGCAATGGTAAATTCAAAACATACAGCTCCACACGTTACTTTAATGGACGAAATCGA
AGTAACTGCACTTATGGCTCATCGCAAACGTTTCAAAGAAGTGGCTGCCGAAAAAGGTATCAAACTAACTTTCTTACCTT
ATATGGTGAAAGCTCTTGTTGCAACGCTTCGTGACTTCCCAGTGCTTAACACAACTTTAGATGATGCGACAGAAGAACTT
GTTTACAAACATTACTTCAACGTTGGTATCGCAGCAGATACAGACCACGGTTTATATGTACCAGTAATTAAAAATGCTGA
TAAAAAATCCGTATTCCAAATTTCTGATGAAATCAACGAACTAGCTGGAAAAGCACGTGATGGTAAATTAACTGCTGACG
AAATGCGCCACGGTTCCGCAACTATTTCTAATATCGGTTCTGCCGGTGGACAATGGTTCACTCCAGTAATTAATTACCCA
GAAGTTGCTATCCTAGGTGTTGGTCGTATTGCTCAAAAACCTATCGTAAAAGATGGCGAAATTGTAGCAGCACCAGTACT
TGCTCTTTCCTTGAGCTTTGACCACCGTGTAATTGACGGCGCAACTGCTCAAAAAGCAATGAACAATATTAAACGTTTAT
TAAACGATCCAGAATTATTACTAATGGAGGTGTAA

Upstream 100 bases:

>100_bases
CCGTTTTAAAACTTATGGGGAGTTTTAGAAGGGGAAAGAAATATTTTTATACTAAGGTTCAGTTTATCTGAACCTTAGAA
ATTACAGGAGGCTTAATAAA

Downstream 100 bases:

>100_bases
CAAAATGGTAGTAGGCGATTTTCCAGAAGAAAGAGACACCATAGTCATCGGTGCAGGCCCAGGTGGGTATGTAGCCGCAA
TCCGAGCTGCACAACTCGGA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MAYSFKLPDIGEGIHEGEIVKWFVQPGDKIEEDESLFEVQNDKSVEEITSPVSGTIKEIKVAEGTVATVGQVLVTFDGVE
GHEDDAEEESAAPKAESTESTPAPAQASGKGIFEFKLPDIGEGIHEGEIVKWFIQPGDKVEEDQSIFEVQNDKSVEEITS
PVDGTVKDILVSEGTVATVGQVLVTFEGDFEGEASHESTPESPAEEAELTNNDATSAPVTGGNGTPSSKKDPNGLVIAMP
SVRKYAREKGVNIAEVAGSGKNNRVVKADIDAFLNGEQPAASTTTAQTEEKAAAPKAEKAAAKQPVASSDAYPETREKLT
PTRRAIAKAMVNSKHTAPHVTLMDEIEVTALMAHRKRFKEVAAEKGIKLTFLPYMVKALVATLRDFPVLNTTLDDATEEL
VYKHYFNVGIAADTDHGLYVPVIKNADKKSVFQISDEINELAGKARDGKLTADEMRHGSATISNIGSAGGQWFTPVINYP
EVAILGVGRIAQKPIVKDGEIVAAPVLALSLSFDHRVIDGATAQKAMNNIKRLLNDPELLLMEV

Sequences:

>Translated_544_residues
MAYSFKLPDIGEGIHEGEIVKWFVQPGDKIEEDESLFEVQNDKSVEEITSPVSGTIKEIKVAEGTVATVGQVLVTFDGVE
GHEDDAEEESAAPKAESTESTPAPAQASGKGIFEFKLPDIGEGIHEGEIVKWFIQPGDKVEEDQSIFEVQNDKSVEEITS
PVDGTVKDILVSEGTVATVGQVLVTFEGDFEGEASHESTPESPAEEAELTNNDATSAPVTGGNGTPSSKKDPNGLVIAMP
SVRKYAREKGVNIAEVAGSGKNNRVVKADIDAFLNGEQPAASTTTAQTEEKAAAPKAEKAAAKQPVASSDAYPETREKLT
PTRRAIAKAMVNSKHTAPHVTLMDEIEVTALMAHRKRFKEVAAEKGIKLTFLPYMVKALVATLRDFPVLNTTLDDATEEL
VYKHYFNVGIAADTDHGLYVPVIKNADKKSVFQISDEINELAGKARDGKLTADEMRHGSATISNIGSAGGQWFTPVINYP
EVAILGVGRIAQKPIVKDGEIVAAPVLALSLSFDHRVIDGATAQKAMNNIKRLLNDPELLLMEV
>Mature_543_residues
AYSFKLPDIGEGIHEGEIVKWFVQPGDKIEEDESLFEVQNDKSVEEITSPVSGTIKEIKVAEGTVATVGQVLVTFDGVEG
HEDDAEEESAAPKAESTESTPAPAQASGKGIFEFKLPDIGEGIHEGEIVKWFIQPGDKVEEDQSIFEVQNDKSVEEITSP
VDGTVKDILVSEGTVATVGQVLVTFEGDFEGEASHESTPESPAEEAELTNNDATSAPVTGGNGTPSSKKDPNGLVIAMPS
VRKYAREKGVNIAEVAGSGKNNRVVKADIDAFLNGEQPAASTTTAQTEEKAAAPKAEKAAAKQPVASSDAYPETREKLTP
TRRAIAKAMVNSKHTAPHVTLMDEIEVTALMAHRKRFKEVAAEKGIKLTFLPYMVKALVATLRDFPVLNTTLDDATEELV
YKHYFNVGIAADTDHGLYVPVIKNADKKSVFQISDEINELAGKARDGKLTADEMRHGSATISNIGSAGGQWFTPVINYPE
VAILGVGRIAQKPIVKDGEIVAAPVLALSLSFDHRVIDGATAQKAMNNIKRLLNDPELLLMEV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=35.6818181818182, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI31711992, Length=569, Percent_Identity=26.3620386643234, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI19923748, Length=242, Percent_Identity=35.9504132231405, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI203098816, Length=479, Percent_Identity=25.0521920668058, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=25.1101321585903, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=32.9341317365269, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1786305, Length=539, Percent_Identity=34.8794063079777, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1786946, Length=437, Percent_Identity=30.2059496567506, Blast_Score=206, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17537937, Length=442, Percent_Identity=34.3891402714932, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI25146366, Length=428, Percent_Identity=30.1401869158879, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=27.8761061946903, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=28.1645569620253, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=435, Percent_Identity=31.4942528735632, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=28.5398230088496, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=36.6742596810934, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=34.061135371179, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=31.304347826087, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=31.304347826087, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58355; Mature: 58224

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSFKLPDIGEGIHEGEIVKWFVQPGDKIEEDESLFEVQNDKSVEEITSPVSGTIKEIK
CCEECCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCHHHHHCCHHCCHHHEE
VAEGTVATVGQVLVTFDGVEGHEDDAEEESAAPKAESTESTPAPAQASGKGIFEFKLPDI
ECCCHHHHHHEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
GEGIHEGEIVKWFIQPGDKVEEDQSIFEVQNDKSVEEITSPVDGTVKDILVSEGTVATVG
CCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCHHEEC
QVLVTFEGDFEGEASHESTPESPAEEAELTNNDATSAPVTGGNGTPSSKKDPNGLVIAMP
EEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
SVRKYAREKGVNIAEVAGSGKNNRVVKADIDAFLNGEQPAASTTTAQTEEKAAAPKAEKA
HHHHHHHHHCCEEEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHH
AAKQPVASSDAYPETREKLTPTRRAIAKAMVNSKHTAPHVTLMDEIEVTALMAHRKRFKE
HHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
VAAEKGIKLTFLPYMVKALVATLRDFPVLNTTLDDATEELVYKHYFNVGIAADTDHGLYV
HHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCEEE
PVIKNADKKSVFQISDEINELAGKARDGKLTADEMRHGSATISNIGSAGGQWFTPVINYP
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHCCCCCCEECCCCCCC
EVAILGVGRIAQKPIVKDGEIVAAPVLALSLSFDHRVIDGATAQKAMNNIKRLLNDPELL
CEEEEECCHHHCCCCCCCCCEEEECHHHEEECCCCEECCCHHHHHHHHHHHHHHCCCCEE
LMEV
EEEC
>Mature Secondary Structure 
AYSFKLPDIGEGIHEGEIVKWFVQPGDKIEEDESLFEVQNDKSVEEITSPVSGTIKEIK
CEECCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCHHHHHCCHHCCHHHEE
VAEGTVATVGQVLVTFDGVEGHEDDAEEESAAPKAESTESTPAPAQASGKGIFEFKLPDI
ECCCHHHHHHEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
GEGIHEGEIVKWFIQPGDKVEEDQSIFEVQNDKSVEEITSPVDGTVKDILVSEGTVATVG
CCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCHHEEC
QVLVTFEGDFEGEASHESTPESPAEEAELTNNDATSAPVTGGNGTPSSKKDPNGLVIAMP
EEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
SVRKYAREKGVNIAEVAGSGKNNRVVKADIDAFLNGEQPAASTTTAQTEEKAAAPKAEKA
HHHHHHHHHCCEEEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHH
AAKQPVASSDAYPETREKLTPTRRAIAKAMVNSKHTAPHVTLMDEIEVTALMAHRKRFKE
HHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
VAAEKGIKLTFLPYMVKALVATLRDFPVLNTTLDDATEELVYKHYFNVGIAADTDHGLYV
HHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCEEE
PVIKNADKKSVFQISDEINELAGKARDGKLTADEMRHGSATISNIGSAGGQWFTPVINYP
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHCCCCCCEECCCCCCC
EVAILGVGRIAQKPIVKDGEIVAAPVLALSLSFDHRVIDGATAQKAMNNIKRLLNDPELL
CEEEEECCHHHCCCCCCCCCEEEECHHHEEECCCCEECCCHHHHHHHHHHHHHHCCCCEE
LMEV
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA