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The map label for this gene is hisH

Identifier: 226223194

GI number: 226223194

Start: 606555

End: 607181

Strand: Reverse

Name: hisH

Synonym: Lm4b_00591

Alternate gene names: 226223194

Gene position: 607181-606555 (Counterclockwise)

Preceding gene: 226223195

Following gene: 226223193

Centisome position: 20.85

GC content: 40.67

Gene sequence:

>627_bases
ATGATTGTTATTATTGACTACGATACAGGAAATACGAAAAGCATCAGTAAAGCCCTTGATTTTATCGGGTTACAAAACAA
AATTTCTAGTGATAAAACAGAAATCGCTCAAGCTGATGGTGTTATTTTGCCCGGAGTTGGTGCTTATCCAGAAGCCATGC
AAGAACTCACTCGACGCGGATTAGATAAAACATTAAAAGAAATTGCTACCGCTGGTAAACCAATTCTTGGCGTTTGTCTT
GGTATGCAACTATTACTCGAATCAAGTAACGAACATAGCTATACTAAAGGGCTCGGCCTCATTCCCGGTCATGTCGAAAT
GCTACCAGACGAATCCGAATTTGCCGTTCCGCACATGGGCTGGAATCAATTACAAATCAAACGCACCACCCCGCTTACAC
AAAACATCGCTGGAGAATATGTCTATTACGTTCATTCCTACTATGCCAACTGTCCAGAAGCTTACATTATCGCAACAAGT
GGTTATTCTATTGATATCCCTGGCATGATAAATAACGGCAATATTTACGGAGCACAGTTCCATCCTGAAAAAAGCGGCCA
AATCGGTCTTGAAATTTTAAAAGGTTTTAAGGAGGTCATTCGTTCATGCAAATCTTCCCAGCAATAG

Upstream 100 bases:

>100_bases
AATCGAAGCACTTTTTAAAGCATTCGGTCGTGCTCTTCGTGAAGCAATCACGATTAACCCAGAAATCAAAGGTGTAAATT
CAACTAAAGGGGTCCTGTAA

Downstream 100 bases:

>100_bases
ACTTAAAAAATGGACAGTGCGTCCGTCTCTTCCAAGGCGACTTCTCTAAAAAAACAGTTGTCAATGAAGACCCAATTGCC
CAAGCAAAAGCATTTGCTAC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIVIIDYDTGNTKSISKALDFIGLQNKISSDKTEIAQADGVILPGVGAYPEAMQELTRRGLDKTLKEIATAGKPILGVCL
GMQLLLESSNEHSYTKGLGLIPGHVEMLPDESEFAVPHMGWNQLQIKRTTPLTQNIAGEYVYYVHSYYANCPEAYIIATS
GYSIDIPGMINNGNIYGAQFHPEKSGQIGLEILKGFKEVIRSCKSSQQ

Sequences:

>Translated_208_residues
MIVIIDYDTGNTKSISKALDFIGLQNKISSDKTEIAQADGVILPGVGAYPEAMQELTRRGLDKTLKEIATAGKPILGVCL
GMQLLLESSNEHSYTKGLGLIPGHVEMLPDESEFAVPHMGWNQLQIKRTTPLTQNIAGEYVYYVHSYYANCPEAYIIATS
GYSIDIPGMINNGNIYGAQFHPEKSGQIGLEILKGFKEVIRSCKSSQQ
>Mature_208_residues
MIVIIDYDTGNTKSISKALDFIGLQNKISSDKTEIAQADGVILPGVGAYPEAMQELTRRGLDKTLKEIATAGKPILGVCL
GMQLLLESSNEHSYTKGLGLIPGHVEMLPDESEFAVPHMGWNQLQIKRTTPLTQNIAGEYVYYVHSYYANCPEAYIIATS
GYSIDIPGMINNGNIYGAQFHPEKSGQIGLEILKGFKEVIRSCKSSQQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=201, Percent_Identity=37.3134328358209, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=31.7307692307692, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_LISMC (C1L0J4)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002757301.1
- ProteinModelPortal:   C1L0J4
- SMR:   C1L0J4
- GeneID:   7702488
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_00591
- OMA:   FRGIENG
- ProtClustDB:   PRK13141
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22756; Mature: 22756

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 181-181 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIIDYDTGNTKSISKALDFIGLQNKISSDKTEIAQADGVILPGVGAYPEAMQELTRRG
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LDKTLKEIATAGKPILGVCLGMQLLLESSNEHSYTKGLGLIPGHVEMLPDESEFAVPHMG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCEECCCCCCCCCCCCC
WNQLQIKRTTPLTQNIAGEYVYYVHSYYANCPEAYIIATSGYSIDIPGMINNGNIYGAQF
CCEEEEEECCCCHHHHCCHHHHHHHHHHCCCCCEEEEEECCCEEECCCEEECCEEEEEEE
HPEKSGQIGLEILKGFKEVIRSCKSSQQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIVIIDYDTGNTKSISKALDFIGLQNKISSDKTEIAQADGVILPGVGAYPEAMQELTRRG
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LDKTLKEIATAGKPILGVCLGMQLLLESSNEHSYTKGLGLIPGHVEMLPDESEFAVPHMG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCEECCCCCCCCCCCCC
WNQLQIKRTTPLTQNIAGEYVYYVHSYYANCPEAYIIATSGYSIDIPGMINNGNIYGAQF
CCEEEEEECCCCHHHHCCHHHHHHHHHHCCCCCEEEEEECCCEEECCCEEECCEEEEEEE
HPEKSGQIGLEILKGFKEVIRSCKSSQQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA