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The map label for this gene is hisA

Identifier: 226223193

GI number: 226223193

Start: 605854

End: 606576

Strand: Reverse

Name: hisA

Synonym: Lm4b_00590

Alternate gene names: 226223193

Gene position: 606576-605854 (Counterclockwise)

Preceding gene: 226223194

Following gene: 226223192

Centisome position: 20.83

GC content: 42.88

Gene sequence:

>723_bases
ATGCAAATCTTCCCAGCAATAGACTTAAAAAATGGACAGTGCGTCCGTCTCTTCCAAGGCGACTTCTCTAAAAAAACAGT
TGTCAATGAAGACCCAATTGCCCAAGCAAAAGCATTTGCTACAGACGGCGCAACCTATTTGCACATCGTCGACCTAGACG
GTGCATTAGAAGGACGTCCTATCAATCTAGAAATTATTCAAAGGATGAAAAAAGCAGCTAAAATCCCTGTTCAAGTTGGT
GGAGGAATTCGTAGCATGGCGCAAGTGGATTATTATCTTGAATCCGGCATCGACCGCGTGATTATCGGTTCTGCCGCTCT
AACAGATCCAGATTTCCTACGTGCGGCAGTCCAAAAATACGGAGCTAAAATTGTAGCAGGTATCGATGCCAAAAACGGTT
TTGTGGCAACAAGAGGTTGGCTCGATGTCAGTCAGGTAAGCTATTTAGATTTGGCTAAACGAATGGAAAAAGTGGGTGTT
GAGACAATCATTTACACTGATATTAGCCGTGATGGCACGCTAACTGGACCTAATTTAGAACAAATGGCAAATCTCAAAGA
ACACGTTAAAGTTAGTTTAATTGCTTCTGGCGGAGTGAGCAGTCGTGCAGATTTGGAAGCACTCGCCCAACTCGGCTTAT
ATGGAGCAATTGCCGGAAAAGCCCTTTATAATCATGATATTTCCATGTCAGATATTGTAGAGGTGGAACAAATTGCTTAC
TAA

Upstream 100 bases:

>100_bases
TGATAAATAACGGCAATATTTACGGAGCACAGTTCCATCCTGAAAAAAGCGGCCAAATCGGTCTTGAAATTTTAAAAGGT
TTTAAGGAGGTCATTCGTTC

Downstream 100 bases:

>100_bases
AAGAATCATCCCATGTCTTGATGTCACAGCAGGTCGAGTCGTCAAAGGTGTTAATTTTGTTTCCTTAACAGATGTCGGGG
ACCCTGTCGAAATTGCTAAA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MQIFPAIDLKNGQCVRLFQGDFSKKTVVNEDPIAQAKAFATDGATYLHIVDLDGALEGRPINLEIIQRMKKAAKIPVQVG
GGIRSMAQVDYYLESGIDRVIIGSAALTDPDFLRAAVQKYGAKIVAGIDAKNGFVATRGWLDVSQVSYLDLAKRMEKVGV
ETIIYTDISRDGTLTGPNLEQMANLKEHVKVSLIASGGVSSRADLEALAQLGLYGAIAGKALYNHDISMSDIVEVEQIAY

Sequences:

>Translated_240_residues
MQIFPAIDLKNGQCVRLFQGDFSKKTVVNEDPIAQAKAFATDGATYLHIVDLDGALEGRPINLEIIQRMKKAAKIPVQVG
GGIRSMAQVDYYLESGIDRVIIGSAALTDPDFLRAAVQKYGAKIVAGIDAKNGFVATRGWLDVSQVSYLDLAKRMEKVGV
ETIIYTDISRDGTLTGPNLEQMANLKEHVKVSLIASGGVSSRADLEALAQLGLYGAIAGKALYNHDISMSDIVEVEQIAY
>Mature_240_residues
MQIFPAIDLKNGQCVRLFQGDFSKKTVVNEDPIAQAKAFATDGATYLHIVDLDGALEGRPINLEIIQRMKKAAKIPVQVG
GGIRSMAQVDYYLESGIDRVIIGSAALTDPDFLRAAVQKYGAKIVAGIDAKNGFVATRGWLDVSQVSYLDLAKRMEKVGV
ETIIYTDISRDGTLTGPNLEQMANLKEHVKVSLIASGGVSSRADLEALAQLGLYGAIAGKALYNHDISMSDIVEVEQIAY

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=35.2459016393443, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=28.8065843621399, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_LISMC (C1L0J3)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002757300.1
- ProteinModelPortal:   C1L0J3
- SMR:   C1L0J3
- GeneID:   7702492
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_00590
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25889; Mature: 25889

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIFPAIDLKNGQCVRLFQGDFSKKTVVNEDPIAQAKAFATDGATYLHIVDLDGALEGRP
CCCCCCEECCCCCEEEEECCCCCCCEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCC
INLEIIQRMKKAAKIPVQVGGGIRSMAQVDYYLESGIDRVIIGSAALTDPDFLRAAVQKY
CCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GAKIVAGIDAKNGFVATRGWLDVSQVSYLDLAKRMEKVGVETIIYTDISRDGTLTGPNLE
CCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHH
QMANLKEHVKVSLIASGGVSSRADLEALAQLGLYGAIAGKALYNHDISMSDIVEVEQIAY
HHHCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQIFPAIDLKNGQCVRLFQGDFSKKTVVNEDPIAQAKAFATDGATYLHIVDLDGALEGRP
CCCCCCEECCCCCEEEEECCCCCCCEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCC
INLEIIQRMKKAAKIPVQVGGGIRSMAQVDYYLESGIDRVIIGSAALTDPDFLRAAVQKY
CCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GAKIVAGIDAKNGFVATRGWLDVSQVSYLDLAKRMEKVGVETIIYTDISRDGTLTGPNLE
CCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHH
QMANLKEHVKVSLIASGGVSSRADLEALAQLGLYGAIAGKALYNHDISMSDIVEVEQIAY
HHHCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA