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The map label for this gene is gdh [H]

Identifier: 226223189

GI number: 226223189

Start: 602614

End: 603990

Strand: Reverse

Name: gdh [H]

Synonym: Lm4b_00586

Alternate gene names: 226223189

Gene position: 603990-602614 (Counterclockwise)

Preceding gene: 226223190

Following gene: 226223187

Centisome position: 20.74

GC content: 42.85

Gene sequence:

>1377_bases
ATGGCACAAACATCCACTATTCAAAATGACACAAAGGCAGCCGAAGAATACGCAGCTCGCGTGTTTGAAACAATTAAACT
ACGGAATCCTGGCGAAACGGAATTCCACCAAGCAGTTGAAGAATTCTTAAACTCTGTTATCCCAGCCCTTGCAAAAGAAC
CTAAATACGAAGCAAATGGTATTTTAGAACAGTTAACAGAACCCGAACGCCTTATCAGTTTCCGAGTTCCATGGGTGGAT
GATTCTGGCACAGTACATGTAAATCGCGGTTACCGTGTCCAATTCAATAGCGCAATTGGTCCATATAAAGGCGGTCTTCG
TTTCCACCCTTCCGTTACTGGAAGCATCGTCAAATTCCTCGGTTTTGAACAAATCTTCAAGAACTCCCTAACTGGCTTAC
CAATCGGTGGCGGTAAAGGTGGCTCTGACTTTGATCCAAAAGGAAAATCAGACTCAGAAGTAATGCGTTTCTGCCAAAGT
TTTATGACTGAACTACAAAAACATATCGGTCCAGATACAGATGTTCCTGCTGGTGATATTGGTGTTGGTGGTCGTGAAAT
TGGCTACTTATTCGGTCAATACAAACGTCTTCGCGGTGCGTATGATGCCGGTACAATTACAGGTAAAGGCCTTTCTTACG
GCGGAAGTTTAGCTCGTACAGAAGCAACCGGTTACGGTCTTGTTTATTTCACCGTTGAAATGTTAGAAGCAGCCGGTGAA
ACCATTCGCGGCAAAAAAATCGTCGTTTCTGGTTCAGGTAACGTTGCGATTTATGCAATCGAAAAAGCACACGAATTGGG
CGCTAAAGTAGTTGCATGTAGCGATTCTGCTGGTTTTGTTTATGACAAAGAAGGTATCAAAGTTGAAACAGTAAAACAAT
TAAAAGAAGTAGAACGTAAACGTATTAGCGAATACACAACCATTCATCCATCTGCAGAATATTACGCAGGCGGCGACGTA
TGGTCCGTTCCATGTGATATCGCACTACCATGTGCAACTCAAAATGAAATCAACGCTGACCAAGCTCGTGCCCTTGTAAA
AAATGGTGTTATCGCTGTTGCAGAAGGCGCAAACATGCCTTCTACTCTTGATGCAGTGGATATTTACCACGAAAATAAAG
TTCTATTCGGTCCTGCAAAAGCTGCCAATGCTGGTGGTGTTGCCGTATCAGCACTTGAAATGGCACAAAACAGCACACGT
ATGAGCTGGACTTTCCAAACAGTCGACGAACATTTACAAAACATCATGAAAGATATTTATAAAAATTCAAGCAATGCTGC
AAGCGAGTATGGCGCACCTGGTAACCTAGTTATCGGCGCAAATATCGCCGGATTCCTCAAAGTAGCAGACACAATGATTT
CTCAAGGTATTATTTAA

Upstream 100 bases:

>100_bases
CAAGCCGATGTTCTTCTTGCTTTATAACATCACGTGTAGTTACATTAAGTTTTCTACTTTTCCTTATAGTCAAAATATTT
AAACAAGGAGCGATTATAGT

Downstream 100 bases:

>100_bases
TTCAAAAAGCCCTTCTTTCTATCCGAAAGAAGGGCTTTTTTGTTATCTCATTAATTTTCGTAAAATAAAGGAAAGTCCGA
ACCATAGAATCACACTAATA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAQTSTIQNDTKAAEEYAARVFETIKLRNPGETEFHQAVEEFLNSVIPALAKEPKYEANGILEQLTEPERLISFRVPWVD
DSGTVHVNRGYRVQFNSAIGPYKGGLRFHPSVTGSIVKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDSEVMRFCQS
FMTELQKHIGPDTDVPAGDIGVGGREIGYLFGQYKRLRGAYDAGTITGKGLSYGGSLARTEATGYGLVYFTVEMLEAAGE
TIRGKKIVVSGSGNVAIYAIEKAHELGAKVVACSDSAGFVYDKEGIKVETVKQLKEVERKRISEYTTIHPSAEYYAGGDV
WSVPCDIALPCATQNEINADQARALVKNGVIAVAEGANMPSTLDAVDIYHENKVLFGPAKAANAGGVAVSALEMAQNSTR
MSWTFQTVDEHLQNIMKDIYKNSSNAASEYGAPGNLVIGANIAGFLKVADTMISQGII

Sequences:

>Translated_458_residues
MAQTSTIQNDTKAAEEYAARVFETIKLRNPGETEFHQAVEEFLNSVIPALAKEPKYEANGILEQLTEPERLISFRVPWVD
DSGTVHVNRGYRVQFNSAIGPYKGGLRFHPSVTGSIVKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDSEVMRFCQS
FMTELQKHIGPDTDVPAGDIGVGGREIGYLFGQYKRLRGAYDAGTITGKGLSYGGSLARTEATGYGLVYFTVEMLEAAGE
TIRGKKIVVSGSGNVAIYAIEKAHELGAKVVACSDSAGFVYDKEGIKVETVKQLKEVERKRISEYTTIHPSAEYYAGGDV
WSVPCDIALPCATQNEINADQARALVKNGVIAVAEGANMPSTLDAVDIYHENKVLFGPAKAANAGGVAVSALEMAQNSTR
MSWTFQTVDEHLQNIMKDIYKNSSNAASEYGAPGNLVIGANIAGFLKVADTMISQGII
>Mature_457_residues
AQTSTIQNDTKAAEEYAARVFETIKLRNPGETEFHQAVEEFLNSVIPALAKEPKYEANGILEQLTEPERLISFRVPWVDD
SGTVHVNRGYRVQFNSAIGPYKGGLRFHPSVTGSIVKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDSEVMRFCQSF
MTELQKHIGPDTDVPAGDIGVGGREIGYLFGQYKRLRGAYDAGTITGKGLSYGGSLARTEATGYGLVYFTVEMLEAAGET
IRGKKIVVSGSGNVAIYAIEKAHELGAKVVACSDSAGFVYDKEGIKVETVKQLKEVERKRISEYTTIHPSAEYYAGGDVW
SVPCDIALPCATQNEINADQARALVKNGVIAVAEGANMPSTLDAVDIYHENKVLFGPAKAANAGGVAVSALEMAQNSTRM
SWTFQTVDEHLQNIMKDIYKNSSNAASEYGAPGNLVIGANIAGFLKVADTMISQGII

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=370, Percent_Identity=32.7027027027027, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4885281, Length=370, Percent_Identity=32.1621621621622, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1788059, Length=440, Percent_Identity=57.7272727272727, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17544676, Length=426, Percent_Identity=27.4647887323944, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6319256, Length=458, Percent_Identity=52.1834061135371, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6324951, Length=450, Percent_Identity=50.8888888888889, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24649283, Length=390, Percent_Identity=30.7692307692308, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45549226, Length=366, Percent_Identity=30.6010928961749, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553475, Length=408, Percent_Identity=29.4117647058824, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24649460, Length=293, Percent_Identity=30.3754266211604, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49252; Mature: 49121

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTSTIQNDTKAAEEYAARVFETIKLRNPGETEFHQAVEEFLNSVIPALAKEPKYEANG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ILEQLTEPERLISFRVPWVDDSGTVHVNRGYRVQFNSAIGPYKGGLRFHPSVTGSIVKFL
HHHHHCCCCHHEEEECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHH
GFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDSEVMRFCQSFMTELQKHIGPDTDVPAGDI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GVGGREIGYLFGQYKRLRGAYDAGTITGKGLSYGGSLARTEATGYGLVYFTVEMLEAAGE
CCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEECCCCCEEHHHHHHHHHHCCC
TIRGKKIVVSGSGNVAIYAIEKAHELGAKVVACSDSAGFVYDKEGIKVETVKQLKEVERK
CCCCCEEEEECCCCEEEEEECCHHHCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHH
RISEYTTIHPSAEYYAGGDVWSVPCDIALPCATQNEINADQARALVKNGVIAVAEGANMP
HHHHHEEECCCCCEECCCCEEECCCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCC
STLDAVDIYHENKVLFGPAKAANAGGVAVSALEMAQNSTRMSWTFQTVDEHLQNIMKDIY
CCHHHHEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
KNSSNAASEYGAPGNLVIGANIAGFLKVADTMISQGII
CCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQTSTIQNDTKAAEEYAARVFETIKLRNPGETEFHQAVEEFLNSVIPALAKEPKYEANG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ILEQLTEPERLISFRVPWVDDSGTVHVNRGYRVQFNSAIGPYKGGLRFHPSVTGSIVKFL
HHHHHCCCCHHEEEECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHH
GFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDSEVMRFCQSFMTELQKHIGPDTDVPAGDI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GVGGREIGYLFGQYKRLRGAYDAGTITGKGLSYGGSLARTEATGYGLVYFTVEMLEAAGE
CCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEECCCCCEEHHHHHHHHHHCCC
TIRGKKIVVSGSGNVAIYAIEKAHELGAKVVACSDSAGFVYDKEGIKVETVKQLKEVERK
CCCCCEEEEECCCCEEEEEECCHHHCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHH
RISEYTTIHPSAEYYAGGDVWSVPCDIALPCATQNEINADQARALVKNGVIAVAEGANMP
HHHHHEEECCCCCEECCCCEEECCCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCC
STLDAVDIYHENKVLFGPAKAANAGGVAVSALEMAQNSTRMSWTFQTVDEHLQNIMKDIY
CCHHHHEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHH
KNSSNAASEYGAPGNLVIGANIAGFLKVADTMISQGII
CCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]