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The map label for this gene is fruA [H]

Identifier: 226223052

GI number: 226223052

Start: 451515

End: 451979

Strand: Direct

Name: fruA [H]

Synonym: Lm4b_00446

Alternate gene names: 226223052

Gene position: 451515-451979 (Clockwise)

Preceding gene: 226223051

Following gene: 226223053

Centisome position: 15.5

GC content: 36.77

Gene sequence:

>465_bases
ATGGAAGTAAAAGATATTTTAACAAAAGAATTAGTCGTCTTTGATTTAGAGGCGACGACAAAAGAAGCAGCAATTGAAGA
AATGGCAGAAATGCTAGATGAACATGGTATTTTAGCGGATAAAGCGACTTATGTTCGGGCAGTTTTGGATCGCGAAGCAC
ATTCAACAACTGGAATAGGAAATAACATTGCTATCCCACACGGAAAAAGTGAAAGTGTCACAAAATCGGCCATCGTTTTT
GCAAGAACGAAAGAAATGATTGAGTGGGAATCACTGGATGACGAACCGGTAAATATGATTTTCTTACTGGCGATTACTGA
TTCTGACAAAACAGACGGTCACTTAAAAATCCTTGCAGAAATTGCAACGAAACTAATGGACGATGATATTGTCGAAAATT
TAAAAAATACAAGAGACGAAGAAGAAGTTATTCGAATTTTAAATGGAGGCGTAGTAGAGATATGA

Upstream 100 bases:

>100_bases
AGTTTTATAAATATATTGATAATAAAAAATGGCTTGAGAAGCTGACACAACTAAAAAGTGCCGCAGAAATTTATGAGCAT
TTGTTAAAGGATGGGATGTA

Downstream 100 bases:

>100_bases
AACGTAAAATTATCGCAGTAACCGCATGTGCGACAGGAGTGGCGCACACATATATGGCAGCACAAGCACTTAAAAAAGGC
GCGAAAAAAATGGGCAATCT

Product: PTS fructose-specific enzyme IIA component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MEVKDILTKELVVFDLEATTKEAAIEEMAEMLDEHGILADKATYVRAVLDREAHSTTGIGNNIAIPHGKSESVTKSAIVF
ARTKEMIEWESLDDEPVNMIFLLAITDSDKTDGHLKILAEIATKLMDDDIVENLKNTRDEEEVIRILNGGVVEI

Sequences:

>Translated_154_residues
MEVKDILTKELVVFDLEATTKEAAIEEMAEMLDEHGILADKATYVRAVLDREAHSTTGIGNNIAIPHGKSESVTKSAIVF
ARTKEMIEWESLDDEPVNMIFLLAITDSDKTDGHLKILAEIATKLMDDDIVENLKNTRDEEEVIRILNGGVVEI
>Mature_154_residues
MEVKDILTKELVVFDLEATTKEAAIEEMAEMLDEHGILADKATYVRAVLDREAHSTTGIGNNIAIPHGKSESVTKSAIVF
ARTKEMIEWESLDDEPVNMIFLLAITDSDKTDGHLKILAEIATKLMDDDIVENLKNTRDEEEVIRILNGGVVEI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=31.7567567567568, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367327, Length=142, Percent_Identity=26.7605633802817, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1786951, Length=149, Percent_Identity=26.1744966442953, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1790027, Length=146, Percent_Identity=30.8219178082192, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1788494, Length=152, Percent_Identity=30.9210526315789, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1788726, Length=146, Percent_Identity=28.0821917808219, Blast_Score=63, Evalue=7e-12,
Organism=Escherichia coli, GI48994992, Length=146, Percent_Identity=26.7123287671233, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17123; Mature: 17123

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKDILTKELVVFDLEATTKEAAIEEMAEMLDEHGILADKATYVRAVLDREAHSTTGIG
CCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
NNIAIPHGKSESVTKSAIVFARTKEMIEWESLDDEPVNMIFLLAITDSDKTDGHLKILAE
CCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
IATKLMDDDIVENLKNTRDEEEVIRILNGGVVEI
HHHHHHCHHHHHHHHCCCCHHHHHHHHCCCEECC
>Mature Secondary Structure
MEVKDILTKELVVFDLEATTKEAAIEEMAEMLDEHGILADKATYVRAVLDREAHSTTGIG
CCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
NNIAIPHGKSESVTKSAIVFARTKEMIEWESLDDEPVNMIFLLAITDSDKTDGHLKILAE
CCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
IATKLMDDDIVENLKNTRDEEEVIRILNGGVVEI
HHHHHHCHHHHHHHHCCCCHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]