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The map label for this gene is frwB [H]

Identifier: 226223053

GI number: 226223053

Start: 451976

End: 452296

Strand: Direct

Name: frwB [H]

Synonym: Lm4b_00447

Alternate gene names: 226223053

Gene position: 451976-452296 (Clockwise)

Preceding gene: 226223052

Following gene: 226223054

Centisome position: 15.52

GC content: 39.25

Gene sequence:

>321_bases
ATGAAACGTAAAATTATCGCAGTAACCGCATGTGCGACAGGAGTGGCGCACACATATATGGCAGCACAAGCACTTAAAAA
AGGCGCGAAAAAAATGGGCAATCTTATCAAAGTAGAAACACAAGGCGCAACAGGAATTGAGAATGAATTAACCGAAAAAG
ATGTAAATATCGGCGAAGTAGTTATTTTCGCAGTAGATACAAAAGTTCGTAACAAAGAACGTTTTGATGGTAAAGTCGTG
CTGGAAGTTCCAGTAAGCGCACCGATTAAAGATGCAGAAAAAGTAATCAACGCAGCACTTGCGTTAATTGACGAAAAATA
A

Upstream 100 bases:

>100_bases
TTGCAACGAAACTAATGGACGATGATATTGTCGAAAATTTAAAAAATACAAGAGACGAAGAAGAAGTTATTCGAATTTTA
AATGGAGGCGTAGTAGAGAT

Downstream 100 bases:

>100_bases
GGAGGATCTACCATGTTCAGAAAAATTGGGAGTGAACTGAAAAAACACGTTTTAACAGGTATTTCATATATGATTCCGCT
CGTTATTGCAGGCGCCGTGA

Product: PTS fructose-specific enzyme IIB component

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MKRKIIAVTACATGVAHTYMAAQALKKGAKKMGNLIKVETQGATGIENELTEKDVNIGEVVIFAVDTKVRNKERFDGKVV
LEVPVSAPIKDAEKVINAALALIDEK

Sequences:

>Translated_106_residues
MKRKIIAVTACATGVAHTYMAAQALKKGAKKMGNLIKVETQGATGIENELTEKDVNIGEVVIFAVDTKVRNKERFDGKVV
LEVPVSAPIKDAEKVINAALALIDEK
>Mature_106_residues
MKRKIIAVTACATGVAHTYMAAQALKKGAKKMGNLIKVETQGATGIENELTEKDVNIGEVVIFAVDTKVRNKERFDGKVV
LEVPVSAPIKDAEKVINAALALIDEK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=51.063829787234, Blast_Score=97, Evalue=1e-22,
Organism=Escherichia coli, GI1786951, Length=98, Percent_Identity=47.9591836734694, Blast_Score=96, Evalue=3e-22,
Organism=Escherichia coli, GI1788730, Length=96, Percent_Identity=42.7083333333333, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI87082348, Length=96, Percent_Identity=38.5416666666667, Blast_Score=83, Evalue=3e-18,
Organism=Escherichia coli, GI1790390, Length=96, Percent_Identity=37.5, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1788492, Length=77, Percent_Identity=46.7532467532467, Blast_Score=72, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 11378; Mature: 11378

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKIIAVTACATGVAHTYMAAQALKKGAKKMGNLIKVETQGATGIENELTEKDVNIGEV
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCCCEE
VIFAVDTKVRNKERFDGKVVLEVPVSAPIKDAEKVINAALALIDEK
EEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRKIIAVTACATGVAHTYMAAQALKKGAKKMGNLIKVETQGATGIENELTEKDVNIGEV
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCCCEE
VIFAVDTKVRNKERFDGKVVLEVPVSAPIKDAEKVINAALALIDEK
EEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]