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The map label for this gene is frwB [H]

Identifier: 226223029

GI number: 226223029

Start: 426939

End: 427271

Strand: Direct

Name: frwB [H]

Synonym: Lm4b_00423

Alternate gene names: 226223029

Gene position: 426939-427271 (Clockwise)

Preceding gene: 226223028

Following gene: 226223030

Centisome position: 14.66

GC content: 45.35

Gene sequence:

>333_bases
ATGAAAGTTGTAGGAGTTACATCATGTATCGCAGGACTTGCGCACACACCAATGGCAGCCAAAGCACTAGAAAAAGCAGG
AGTAAAATTAGGACACGATGTCAAAATTGAGCAACAAGGAGCGATGGGGACAATTGATGAAATCACGCCAGCTGAAGTGA
GCGCGGCAGATGTAGTTATCATCGCGGCGGATAAAGTAATCGACGGGGAAGAGCGTTTCAAAGGAAAACCAGTCGTTCGT
GTGAAAATTGGTCAATGTGTTGCGAACGGGGAAGCGGTGTTAAGTAAAGTAGTTGCAGCAATTGAAGCACGTAAACAAAA
GGAGGCTAACTAA

Upstream 100 bases:

>100_bases
TGAATTTACTAGAAGAAGCATTTCAAGAACAAATTTTAACGATTAAAACGGAACACGAATTGATGGATTACTTGAAAGAT
AATTTGGAAGGAGAATTAAT

Downstream 100 bases:

>100_bases
GTCATGAAAGGGAAATTAAACGAAGCAAAAGACCACTTAATGTCGGGTATATCCTATGCGCTACCAGTCATTATCGCAGG
TTCACTTGTTGTAGCTGTTG

Product: fructose-specific phosphotransferase enzyme IIB

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MKVVGVTSCIAGLAHTPMAAKALEKAGVKLGHDVKIEQQGAMGTIDEITPAEVSAADVVIIAADKVIDGEERFKGKPVVR
VKIGQCVANGEAVLSKVVAAIEARKQKEAN

Sequences:

>Translated_110_residues
MKVVGVTSCIAGLAHTPMAAKALEKAGVKLGHDVKIEQQGAMGTIDEITPAEVSAADVVIIAADKVIDGEERFKGKPVVR
VKIGQCVANGEAVLSKVVAAIEARKQKEAN
>Mature_110_residues
MKVVGVTSCIAGLAHTPMAAKALEKAGVKLGHDVKIEQQGAMGTIDEITPAEVSAADVVIIAADKVIDGEERFKGKPVVR
VKIGQCVANGEAVLSKVVAAIEARKQKEAN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=46.9387755102041, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI87082348, Length=99, Percent_Identity=38.3838383838384, Blast_Score=79, Evalue=8e-17,
Organism=Escherichia coli, GI1786951, Length=96, Percent_Identity=41.6666666666667, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1788492, Length=98, Percent_Identity=45.9183673469388, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1788730, Length=105, Percent_Identity=32.3809523809524, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1790390, Length=104, Percent_Identity=35.5769230769231, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 11388; Mature: 11388

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVGVTSCIAGLAHTPMAAKALEKAGVKLGHDVKIEQQGAMGTIDEITPAEVSAADVVI
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEE
IAADKVIDGEERFKGKPVVRVKIGQCVANGEAVLSKVVAAIEARKQKEAN
EECCCEECCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVVGVTSCIAGLAHTPMAAKALEKAGVKLGHDVKIEQQGAMGTIDEITPAEVSAADVVI
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEE
IAADKVIDGEERFKGKPVVRVKIGQCVANGEAVLSKVVAAIEARKQKEAN
EECCCEECCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]