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The map label for this gene is fruA [H]

Identifier: 226223030

GI number: 226223030

Start: 427275

End: 428387

Strand: Direct

Name: fruA [H]

Synonym: Lm4b_00424

Alternate gene names: 226223030

Gene position: 427275-428387 (Clockwise)

Preceding gene: 226223029

Following gene: 226223031

Centisome position: 14.67

GC content: 42.05

Gene sequence:

>1113_bases
ATGAAAGGGAAATTAAACGAAGCAAAAGACCACTTAATGTCGGGTATATCCTATGCGCTACCAGTCATTATCGCAGGTTC
ACTTGTTGTAGCTGTTGCAAAATTGATTGGGATTATTGGCGGGGTTACAAATCTGGATGCTTATGCTGATGCAAGCGGAT
TTTATCATTATGTTTATCTATTTCAAAATGTAGGTTGGGCGGCAATTGGCTTACTTAACTTAGTACTTGCGGGATATATC
GCTTATTCAATCGCTGGAAAACCTGCTCTTGCTGCTGGTTTTGTTGGTGGGGTCCTTGCTACTAGTACAAATGCTGGTTT
CTTAGGTGCCGTTGTCGCTGGTTTCTTCGCAGGTTGGTTAACAAACTGGGTGAAAAAACATGTTCGAATCACTGGACCTG
CTGCAAGTTCTTTACCACTAATTATTTTGCCACTTATCACAGTTGGTGCCACGGGGATACTGATGTCACTAATTCTCGGC
GGACCGCTTGGTTGGTTAAACCAAACGTTACTTGATTGGGTAACTGAAATGTGTAAAAACGATACAAACGTCATTATTCT
AGCACTTATTTTAGGAGCAATGATTGGATTTGACCTTGGTGGACCTGTAAACAAAGCTGCTTGGATGGCTGGGAACGCGC
TATTTATGAGTGGAATTTATCTACCTTCAATCATGATCAACGTCGCAATTTGCATTCCGCCACTTGGCTACGGAATCGCA
ACACTTTTACGCAAAAAGAATTATTCTACTACATTCAAAGAAGCTGGAAATGGCGCGCTAATCATGGGACTAATTGGGGT
CACGGAAGGTGCGATTCCTTTTACACTGCGTAGCCCTGGTAAATTAATTCCGTTAAATGTGATTGCCTGTGCGATTGGTA
GTGCAGTAACAATGGGGCTTGGCGCTTATGTGAAAATGCCGCCGATTGGTGGAATGTACGGTTTCTTCACTATTGGTAAC
GGCTGGGCTTACTTAGTTGGTGGCTTGGTTGGTGCATTTATTATTGGAATTTGTGCGAACTTATTCGTCAATTTCACAGA
AGAAGAAACAGCTGCTGCAGACGATGCTGTGGATGATATCGATATTAGTTTTGATGAAATAGAGATTAAATAA

Upstream 100 bases:

>100_bases
TCGTGTGAAAATTGGTCAATGTGTTGCGAACGGGGAAGCGGTGTTAAGTAAAGTAGTTGCAGCAATTGAAGCACGTAAAC
AAAAGGAGGCTAACTAAGTC

Downstream 100 bases:

>100_bases
TAGTTGGAGGATTTAATGAAAATGGCTAAAACGAAAGTACACGTAATTCCGCATTCGCACTGGGATAGAGAATGGTATTT
CACTTCAAGCCGTTCAACAA

Product: fructose-specific phosphotransferase enzyme IIC

Products: NA

Alternate protein names: EIIBBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKGKLNEAKDHLMSGISYALPVIIAGSLVVAVAKLIGIIGGVTNLDAYADASGFYHYVYLFQNVGWAAIGLLNLVLAGYI
AYSIAGKPALAAGFVGGVLATSTNAGFLGAVVAGFFAGWLTNWVKKHVRITGPAASSLPLIILPLITVGATGILMSLILG
GPLGWLNQTLLDWVTEMCKNDTNVIILALILGAMIGFDLGGPVNKAAWMAGNALFMSGIYLPSIMINVAICIPPLGYGIA
TLLRKKNYSTTFKEAGNGALIMGLIGVTEGAIPFTLRSPGKLIPLNVIACAIGSAVTMGLGAYVKMPPIGGMYGFFTIGN
GWAYLVGGLVGAFIIGICANLFVNFTEEETAAADDAVDDIDISFDEIEIK

Sequences:

>Translated_370_residues
MKGKLNEAKDHLMSGISYALPVIIAGSLVVAVAKLIGIIGGVTNLDAYADASGFYHYVYLFQNVGWAAIGLLNLVLAGYI
AYSIAGKPALAAGFVGGVLATSTNAGFLGAVVAGFFAGWLTNWVKKHVRITGPAASSLPLIILPLITVGATGILMSLILG
GPLGWLNQTLLDWVTEMCKNDTNVIILALILGAMIGFDLGGPVNKAAWMAGNALFMSGIYLPSIMINVAICIPPLGYGIA
TLLRKKNYSTTFKEAGNGALIMGLIGVTEGAIPFTLRSPGKLIPLNVIACAIGSAVTMGLGAYVKMPPIGGMYGFFTIGN
GWAYLVGGLVGAFIIGICANLFVNFTEEETAAADDAVDDIDISFDEIEIK
>Mature_370_residues
MKGKLNEAKDHLMSGISYALPVIIAGSLVVAVAKLIGIIGGVTNLDAYADASGFYHYVYLFQNVGWAAIGLLNLVLAGYI
AYSIAGKPALAAGFVGGVLATSTNAGFLGAVVAGFFAGWLTNWVKKHVRITGPAASSLPLIILPLITVGATGILMSLILG
GPLGWLNQTLLDWVTEMCKNDTNVIILALILGAMIGFDLGGPVNKAAWMAGNALFMSGIYLPSIMINVAICIPPLGYGIA
TLLRKKNYSTTFKEAGNGALIMGLIGVTEGAIPFTLRSPGKLIPLNVIACAIGSAVTMGLGAYVKMPPIGGMYGFFTIGN
GWAYLVGGLVGAFIIGICANLFVNFTEEETAAADDAVDDIDISFDEIEIK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=348, Percent_Identity=34.4827586206897, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1790386, Length=319, Percent_Identity=36.0501567398119, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786951, Length=337, Percent_Identity=34.1246290801187, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082348, Length=309, Percent_Identity=31.3915857605178, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788729, Length=366, Percent_Identity=27.5956284153005, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 38429; Mature: 38429

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKLNEAKDHLMSGISYALPVIIAGSLVVAVAKLIGIIGGVTNLDAYADASGFYHYVYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHH
FQNVGWAAIGLLNLVLAGYIAYSIAGKPALAAGFVGGVLATSTNAGFLGAVVAGFFAGWL
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TNWVKKHVRITGPAASSLPLIILPLITVGATGILMSLILGGPLGWLNQTLLDWVTEMCKN
HHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
DTNVIILALILGAMIGFDLGGPVNKAAWMAGNALFMSGIYLPSIMINVAICIPPLGYGIA
CCCEEHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
TLLRKKNYSTTFKEAGNGALIMGLIGVTEGAIPFTLRSPGKLIPLNVIACAIGSAVTMGL
HHHHHCCCCCHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCC
GAYVKMPPIGGMYGFFTIGNGWAYLVGGLVGAFIIGICANLFVNFTEEETAAADDAVDDI
CCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCCCCC
DISFDEIEIK
CCCCEEEEEC
>Mature Secondary Structure
MKGKLNEAKDHLMSGISYALPVIIAGSLVVAVAKLIGIIGGVTNLDAYADASGFYHYVYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHH
FQNVGWAAIGLLNLVLAGYIAYSIAGKPALAAGFVGGVLATSTNAGFLGAVVAGFFAGWL
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TNWVKKHVRITGPAASSLPLIILPLITVGATGILMSLILGGPLGWLNQTLLDWVTEMCKN
HHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
DTNVIILALILGAMIGFDLGGPVNKAAWMAGNALFMSGIYLPSIMINVAICIPPLGYGIA
CCCEEHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
TLLRKKNYSTTFKEAGNGALIMGLIGVTEGAIPFTLRSPGKLIPLNVIACAIGSAVTMGL
HHHHHCCCCCHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCC
GAYVKMPPIGGMYGFFTIGNGWAYLVGGLVGAFIIGICANLFVNFTEEETAAADDAVDDI
CCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCCCCC
DISFDEIEIK
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2254279 [H]