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The map label for this gene is hrsA [H]

Identifier: 226223028

GI number: 226223028

Start: 426484

End: 426942

Strand: Direct

Name: hrsA [H]

Synonym: Lm4b_00422

Alternate gene names: 226223028

Gene position: 426484-426942 (Clockwise)

Preceding gene: 226223027

Following gene: 226223029

Centisome position: 14.64

GC content: 37.69

Gene sequence:

>459_bases
ATGATAGTATCGGAAGAACAATTATTTTTAAATCAGCATCTACGCACAAAAGAAGAAGTGCTAGCTTTTATTGCAGACAA
GGCAGAAGAAATCGGTATCACAACAAATTCAGCTCAGGTGGAAAGTGACTTATGGGCTCGCGAAAAAGAATACGCAACAG
CAGTACAACAATTAATCGCTATTCCACATGCGAAAACAGAGGCAATATCAGAAGCAAAACTGCTTTTCATTCGCCTCACT
GAACCGATTGATTGGGAGTCAAAAGAAGGTTTTAAGGCACAAGCCATTTTTGCCATATTAGTTCCAGCTAGTGAGGCCAG
CCAGCAACATCTGAAAATTTTATCTAGCGTGGCTGTGAATTTACTAGAAGAAGCATTTCAAGAACAAATTTTAACGATTA
AAACGGAACACGAATTGATGGATTACTTGAAAGATAATTTGGAAGGAGAATTAATATGA

Upstream 100 bases:

>100_bases
TTTTTCCACTTTAACTGGAAATTCCTTATGTTCAAAAAAGTAATCGGTTTCATTAGAATGTAATTGTAAGCAAGGCATTG
AAAAAATACGGGGTGACGAA

Downstream 100 bases:

>100_bases
AAGTTGTAGGAGTTACATCATGTATCGCAGGACTTGCGCACACACCAATGGCAGCCAAAGCACTAGAAAAAGCAGGAGTA
AAATTAGGACACGATGTCAA

Product: phosphotransferase system enzyme IIA

Products: NA

Alternate protein names: Putative PTS system EIIABC component; Phosphotransferase enzyme IIA component; PTS system EIIA component; Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MIVSEEQLFLNQHLRTKEEVLAFIADKAEEIGITTNSAQVESDLWAREKEYATAVQQLIAIPHAKTEAISEAKLLFIRLT
EPIDWESKEGFKAQAIFAILVPASEASQQHLKILSSVAVNLLEEAFQEQILTIKTEHELMDYLKDNLEGELI

Sequences:

>Translated_152_residues
MIVSEEQLFLNQHLRTKEEVLAFIADKAEEIGITTNSAQVESDLWAREKEYATAVQQLIAIPHAKTEAISEAKLLFIRLT
EPIDWESKEGFKAQAIFAILVPASEASQQHLKILSSVAVNLLEEAFQEQILTIKTEHELMDYLKDNLEGELI
>Mature_152_residues
MIVSEEQLFLNQHLRTKEEVLAFIADKAEEIGITTNSAQVESDLWAREKEYATAVQQLIAIPHAKTEAISEAKLLFIRLT
EPIDWESKEGFKAQAIFAILVPASEASQQHLKILSSVAVNLLEEAFQEQILTIKTEHELMDYLKDNLEGELI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786951, Length=145, Percent_Identity=26.8965517241379, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI48994992, Length=147, Percent_Identity=30.6122448979592, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1788726, Length=142, Percent_Identity=26.7605633802817, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17191; Mature: 17191

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSEEQLFLNQHLRTKEEVLAFIADKAEEIGITTNSAQVESDLWAREKEYATAVQQLIA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IPHAKTEAISEAKLLFIRLTEPIDWESKEGFKAQAIFAILVPASEASQQHLKILSSVAVN
CCCCHHHHHHHHEEEEEEECCCCCCCCCCCCHHEEEEEEEECCCHHHHHHHHHHHHHHHH
LLEEAFQEQILTIKTEHELMDYLKDNLEGELI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIVSEEQLFLNQHLRTKEEVLAFIADKAEEIGITTNSAQVESDLWAREKEYATAVQQLIA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IPHAKTEAISEAKLLFIRLTEPIDWESKEGFKAQAIFAILVPASEASQQHLKILSSVAVN
CCCCHHHHHHHHEEEEEEECCCCCCCCCCCCHHEEEEEEEECCCHHHHHHHHHHHHHHHH
LLEEAFQEQILTIKTEHELMDYLKDNLEGELI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9063979; 9278503; 8905232 [H]