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The map label for this gene is fixK [H]

Identifier: 225686224

GI number: 225686224

Start: 378420

End: 379178

Strand: Reverse

Name: fixK [H]

Synonym: BMEA_B0392

Alternate gene names: 225686224

Gene position: 379178-378420 (Counterclockwise)

Preceding gene: 225686228

Following gene: 225686220

Centisome position: 31.98

GC content: 49.8

Gene sequence:

>759_bases
ATGGGGATAAGCAGTGAAGATAATGTCCTGCATAATATATTCGAGAATCAGCCCATCAATTCGGCTTTCAACACAGACGA
TCTGGGCACCCTGAAATCTCTGCCGGTTTCATTGAAACATTATCCGCCCCATACAATTGTTTCGAGACAGGGGGACTGGA
CCAATTCCATCCTTATTATCAATAGCGGCTGGAGCTGTATCTATCGCGATCTGCCCGAAGGTGATCGGCAAATCCTCGAT
TTTCCGATGAAAGGCGATTTTCTCGGTTTTCGGGCGGGGCTTGGTTTCAACTATTACACGCTTTTTGCAATAACGGATCT
TTCCGTTGTTGAAATTTCACTGGATATCCTTGCGGAAAAAATGCGCAAATCACCGCCGCTCGCCGTGACATTCATGGAAC
TGACGGCGCGACAACGCACCATTCTTCTGGAACACCTCATAAACCTTGGGCGAAGGACGTCGCTTGCACGCATCGCGCAT
CTTATGCTGGAGCTTGGTTTCAGGACAAAAGCCAATGGCACGGGAGATGCACACGGCTTCTACTGCCCGCTGACCCAGGG
GGAACTGGCGGATGCACTGGGCCTCACCCCCATTCATATAAACCGGATGCTGCGGGAATTGCGGGAAGACAACCTGCTTG
TCTTCAAGAACAATGAAGTCAGGTTCCTGAACCGCCCGGCATTGATGCAGGTTTGCAGCTTCGACGAGAATTATCTGTCA
GCAAATATCTTCACCAACATCCACTTGATCCCACGATAA

Upstream 100 bases:

>100_bases
CGCTCAAGGAAACTTGCAGCTATGGCAAATAAATTCGGGGGACGGCGTGCCGGCTACGGAACCGTGGCACGGCTCGTCAA
AGTAAGAACCGGGTAAACCT

Downstream 100 bases:

>100_bases
TCACTTGCCGCAAGCTGTTGCTGCTCTTGCGCTGTCAAAAGCGAAATCCGCATCCTGATTGAGACAATATTCTCCACGCC
CAACCCTCAGAAACATCGCA

Product: transcriptional regulator Crp/Fnr family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MGISSEDNVLHNIFENQPINSAFNTDDLGTLKSLPVSLKHYPPHTIVSRQGDWTNSILIINSGWSCIYRDLPEGDRQILD
FPMKGDFLGFRAGLGFNYYTLFAITDLSVVEISLDILAEKMRKSPPLAVTFMELTARQRTILLEHLINLGRRTSLARIAH
LMLELGFRTKANGTGDAHGFYCPLTQGELADALGLTPIHINRMLRELREDNLLVFKNNEVRFLNRPALMQVCSFDENYLS
ANIFTNIHLIPR

Sequences:

>Translated_252_residues
MGISSEDNVLHNIFENQPINSAFNTDDLGTLKSLPVSLKHYPPHTIVSRQGDWTNSILIINSGWSCIYRDLPEGDRQILD
FPMKGDFLGFRAGLGFNYYTLFAITDLSVVEISLDILAEKMRKSPPLAVTFMELTARQRTILLEHLINLGRRTSLARIAH
LMLELGFRTKANGTGDAHGFYCPLTQGELADALGLTPIHINRMLRELREDNLLVFKNNEVRFLNRPALMQVCSFDENYLS
ANIFTNIHLIPR
>Mature_251_residues
GISSEDNVLHNIFENQPINSAFNTDDLGTLKSLPVSLKHYPPHTIVSRQGDWTNSILIINSGWSCIYRDLPEGDRQILDF
PMKGDFLGFRAGLGFNYYTLFAITDLSVVEISLDILAEKMRKSPPLAVTFMELTARQRTILLEHLINLGRRTSLARIAHL
MLELGFRTKANGTGDAHGFYCPLTQGELADALGLTPIHINRMLRELREDNLLVFKNNEVRFLNRPALMQVCSFDENYLSA
NIFTNIHLIPR

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=191, Percent_Identity=27.2251308900524, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28545; Mature: 28414

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISSEDNVLHNIFENQPINSAFNTDDLGTLKSLPVSLKHYPPHTIVSRQGDWTNSILII
CCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCEEECCCCCCCEEEEE
NSGWSCIYRDLPEGDRQILDFPMKGDFLGFRAGLGFNYYTLFAITDLSVVEISLDILAEK
ECCCCEEECCCCCCCHHEEECCCCCCEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHH
MRKSPPLAVTFMELTARQRTILLEHLINLGRRTSLARIAHLMLELGFRTKANGTGDAHGF
HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCE
YCPLTQGELADALGLTPIHINRMLRELREDNLLVFKNNEVRFLNRPALMQVCSFDENYLS
EECCCCCHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEECCHHHHHHHCCCCCCEE
ANIFTNIHLIPR
EEEEEEEEEECC
>Mature Secondary Structure 
GISSEDNVLHNIFENQPINSAFNTDDLGTLKSLPVSLKHYPPHTIVSRQGDWTNSILII
CCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCEEECCCCCCCEEEEE
NSGWSCIYRDLPEGDRQILDFPMKGDFLGFRAGLGFNYYTLFAITDLSVVEISLDILAEK
ECCCCEEECCCCCCCHHEEECCCCCCEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHH
MRKSPPLAVTFMELTARQRTILLEHLINLGRRTSLARIAHLMLELGFRTKANGTGDAHGF
HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCE
YCPLTQGELADALGLTPIHINRMLRELREDNLLVFKNNEVRFLNRPALMQVCSFDENYLS
EECCCCCHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEECCHHHHHHHCCCCCCEE
ANIFTNIHLIPR
EEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]