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The map label for this gene is ahpF [C]

Identifier: 222527426

GI number: 222527426

Start: 5224295

End: 5224957

Strand: Reverse

Name: ahpF [C]

Synonym: Chy400_4217

Alternate gene names: 222527426

Gene position: 5224957-5224295 (Counterclockwise)

Preceding gene: 222527427

Following gene: 222527425

Centisome position: 99.17

GC content: 55.2

Gene sequence:

>663_bases
ATGGCTCTTATGCAGGAAAAAGATCGCAAGGCGGTGCAGGGAGCATTTACAGGTCTGAATCGACCGGTAGACATCGTGCT
CTTCACCTCTGCCGAAAGTGGTGAGTACAGCGAGGTCACCCAGGAACTGTTGCAAGAAGTTGTGGCTCTCCATCCAATGC
TGAGTCTGCACGTCTATGATCTGGATCAGGATAGTGCCTATGCTGCGGAATTGGGTGTTGACAAAGCGCCGGGAATTGTT
TTCCTGGTTGGTGAAGAGCGGCAGAATCACGGCCTGCGCTTTGCCGGATTGCCCAGTGGTTATGAATTTGCTTCCTTAAT
TGAAGCTATCCGTCTGGCCGGTGGTGCGGTACAGCCTGACTTGCAACCGGCAACCATGGCGCTGTTGGAGACGATTCAAT
CCCCAATGCACTTGCAAGTCTTTGTGACGCCAACCTGTCCGTACTGCCCGCGCGCAGTGGTGATGGCGTACCGCCTGGCT
CTGGCCAGTCCGTACATCTCTGCCGAAGGCATCGAAGTGACCGAATTCCCCGAATTGGGCGACCGCTACGCGGTGATGGG
AGTGCCCAAGACCGTCATAGATGATCTCGTGCATATCGAAGGTGCAGTGCCCGAAGGGATGATGGTCAATAAACTGCGGG
AAGCGATTGCTGCGGCGGCGTAA

Upstream 100 bases:

>100_bases
GCTATGAGAGAAGGCCGTGCCTGAGACCGGCAATGTAACTAAACGCACCGGCCTTGCATCTTATTGGTAGTGATGCGAAT
TATTGTGAAAGGAACAACAC

Downstream 100 bases:

>100_bases
TGCCTGCTCTGCGAGAGTAATACCTCTGTAGACTACAGGAAAGCTGGTGCCGTCCTGCTCTCCTATCGGGATAGCTGAAG
TTGATGCTTTCAGCTATCCC

Product: glutaredoxin-like domain-containing protein

Products: an alcohol; H2O; NAD [C]

Alternate protein names: Glutaredoxin-Like Domain-Containing Protein; Glutaredoxin-Like Protein; Alkyl Hydroperoxide Reductase Subunit F; Thioredoxin Reductase; Alkyl Hydroperoxide Reductase F Subunit; FAD-Dependent Pyridine Nucleotide-Disulphide Oxidoreductase; Glutaredoxin-Related Protein; AhpF Family Protein/Thioredoxin Reductase; Protein Disulfide Oxidoreductase; Protein-Disulfide Oxidoreductase; Thioredoxin Reductase/Glutathione-Related Protein; NADH Dehydrogenase; Dehydrogenase Selenocysteine-Containing; Thioredoxin Reductase-Like Protein; Dehydrogenase Selenocysteine-Containing Protein; Thioredoxin-Disulfide Reductase; Alkyl Hydroperoxide Reductase Subunit; Redoxin-Like Protein; Glutaredoxin Related Protein; Alkyl Hydroperoxide Reductase Subunit F Related Protein

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MALMQEKDRKAVQGAFTGLNRPVDIVLFTSAESGEYSEVTQELLQEVVALHPMLSLHVYDLDQDSAYAAELGVDKAPGIV
FLVGEERQNHGLRFAGLPSGYEFASLIEAIRLAGGAVQPDLQPATMALLETIQSPMHLQVFVTPTCPYCPRAVVMAYRLA
LASPYISAEGIEVTEFPELGDRYAVMGVPKTVIDDLVHIEGAVPEGMMVNKLREAIAAAA

Sequences:

>Translated_220_residues
MALMQEKDRKAVQGAFTGLNRPVDIVLFTSAESGEYSEVTQELLQEVVALHPMLSLHVYDLDQDSAYAAELGVDKAPGIV
FLVGEERQNHGLRFAGLPSGYEFASLIEAIRLAGGAVQPDLQPATMALLETIQSPMHLQVFVTPTCPYCPRAVVMAYRLA
LASPYISAEGIEVTEFPELGDRYAVMGVPKTVIDDLVHIEGAVPEGMMVNKLREAIAAAA
>Mature_219_residues
ALMQEKDRKAVQGAFTGLNRPVDIVLFTSAESGEYSEVTQELLQEVVALHPMLSLHVYDLDQDSAYAAELGVDKAPGIVF
LVGEERQNHGLRFAGLPSGYEFASLIEAIRLAGGAVQPDLQPATMALLETIQSPMHLQVFVTPTCPYCPRAVVMAYRLAL
ASPYISAEGIEVTEFPELGDRYAVMGVPKTVIDDLVHIEGAVPEGMMVNKLREAIAAAA

Specific function: Serves To Protect The Cell Against DNA Damage By Alkyl Hydroperoxides. It Can Use Either NADH Or NADPH As Electron Donor For Direct Reduction Of Redox Dyes Or Of Alkyl Hydroperoxides When Combined With The Ahpc Protein. [C]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.6.4.- [C]

Molecular weight: Translated: 23773; Mature: 23641

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALMQEKDRKAVQGAFTGLNRPVDIVLFTSAESGEYSEVTQELLQEVVALHPMLSLHVYD
CCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEE
LDQDSAYAAELGVDKAPGIVFLVGEERQNHGLRFAGLPSGYEFASLIEAIRLAGGAVQPD
CCCCCHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCC
LQPATMALLETIQSPMHLQVFVTPTCPYCPRAVVMAYRLALASPYISAEGIEVTEFPELG
CCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCC
DRYAVMGVPKTVIDDLVHIEGAVPEGMMVNKLREAIAAAA
CCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALMQEKDRKAVQGAFTGLNRPVDIVLFTSAESGEYSEVTQELLQEVVALHPMLSLHVYD
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEE
LDQDSAYAAELGVDKAPGIVFLVGEERQNHGLRFAGLPSGYEFASLIEAIRLAGGAVQPD
CCCCCHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCC
LQPATMALLETIQSPMHLQVFVTPTCPYCPRAVVMAYRLALASPYISAEGIEVTEFPELG
CCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCC
DRYAVMGVPKTVIDDLVHIEGAVPEGMMVNKLREAIAAAA
CCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA