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The map label for this gene is sigW [H]

Identifier: 222527257

GI number: 222527257

Start: 4990038

End: 4990637

Strand: Direct

Name: sigW [H]

Synonym: Chy400_4041

Alternate gene names: 222527257

Gene position: 4990038-4990637 (Clockwise)

Preceding gene: 222527254

Following gene: 222527258

Centisome position: 94.71

GC content: 52.67

Gene sequence:

>600_bases
GTGGCCCAACCGATTGATGCAGCGCATCTGTCACGATTAAGTGATGAATCTCTGATGGAGCTGGTTGCTGCCGGTGATAG
TACAGCGCTCGCTCACCTGTACGACCGCCATTCGCGGGCAGTGTATGGTCTGGCCCTGAGAATGCTGGCCACCGCTGAAG
CGGCTGAAGAGGTTGTACAGGAGACGTTCTGGCGTGTCTGGAAACGAGCGGCCACCTTTCAACGCAACAGTGCGTTTTTG
CCGTGGATGTTCGGTATTGCCCGTAACCTGTGCATTGATGAATTGCGTCGACGTCAGGCCCGACCGGTGGCTGCCAGTAA
CAATGAAGATATTTTGACAACGCTTACTGACCAGCAACAGAACGTTGAGCAATCAACCATTGAAACCGAACGTCGGCGGC
TGATCACCAGCGCTCTGGCCGATTTACCGACCGACCAGCGTGAAGTTATTGAGCTGGCCTATTTTGGTGGCTTATCGCAG
CGAGAAATCGCCGAGTATTTACAGAGTCCGCTTGGCACAATTAAGACGAGAATTCGTTTAGGTTTACAGAAGTTGAAACA
GACCTTGCTTCACTACGGCATCGATGCAAACGACCACTAG

Upstream 100 bases:

>100_bases
AGGGATCAGTGTATAATAAGAATCGGTAATCTTCGCACATCACCGCATTGATAGGCATCCAAATTGACGTGGTGTGCGTA
TATTTCATGTGAGGTTGGCT

Downstream 100 bases:

>100_bases
AAGAGTAGCTGCATGCACGACTCTGACCGCGAACATGAACAATCTCTCCTCGCCGCAGCCGCGCTCGGCAGCCTCGATCC
CACCGATCTGGCAGCGTTCG

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MAQPIDAAHLSRLSDESLMELVAAGDSTALAHLYDRHSRAVYGLALRMLATAEAAEEVVQETFWRVWKRAATFQRNSAFL
PWMFGIARNLCIDELRRRQARPVAASNNEDILTTLTDQQQNVEQSTIETERRRLITSALADLPTDQREVIELAYFGGLSQ
REIAEYLQSPLGTIKTRIRLGLQKLKQTLLHYGIDANDH

Sequences:

>Translated_199_residues
MAQPIDAAHLSRLSDESLMELVAAGDSTALAHLYDRHSRAVYGLALRMLATAEAAEEVVQETFWRVWKRAATFQRNSAFL
PWMFGIARNLCIDELRRRQARPVAASNNEDILTTLTDQQQNVEQSTIETERRRLITSALADLPTDQREVIELAYFGGLSQ
REIAEYLQSPLGTIKTRIRLGLQKLKQTLLHYGIDANDH
>Mature_198_residues
AQPIDAAHLSRLSDESLMELVAAGDSTALAHLYDRHSRAVYGLALRMLATAEAAEEVVQETFWRVWKRAATFQRNSAFLP
WMFGIARNLCIDELRRRQARPVAASNNEDILTTLTDQQQNVEQSTIETERRRLITSALADLPTDQREVIELAYFGGLSQR
EIAEYLQSPLGTIKTRIRLGLQKLKQTLLHYGIDANDH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=172, Percent_Identity=30.2325581395349, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22548; Mature: 22417

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPIDAAHLSRLSDESLMELVAAGDSTALAHLYDRHSRAVYGLALRMLATAEAAEEVVQ
CCCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFWRVWKRAATFQRNSAFLPWMFGIARNLCIDELRRRQARPVAASNNEDILTTLTDQQQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHH
NVEQSTIETERRRLITSALADLPTDQREVIELAYFGGLSQREIAEYLQSPLGTIKTRIRL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GLQKLKQTLLHYGIDANDH
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQPIDAAHLSRLSDESLMELVAAGDSTALAHLYDRHSRAVYGLALRMLATAEAAEEVVQ
CCCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFWRVWKRAATFQRNSAFLPWMFGIARNLCIDELRRRQARPVAASNNEDILTTLTDQQQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHH
NVEQSTIETERRRLITSALADLPTDQREVIELAYFGGLSQREIAEYLQSPLGTIKTRIRL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GLQKLKQTLLHYGIDANDH
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]